Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA
The quantity and quality of DNA are crucial factors in determining polymorphisms in samples from individuals with and without pathologies such as cleft lip and palate (CL/P). Saliva is increasingly being evaluated as a collection method. This study aimed to compare saliva DNA concentrations from ind...
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Elsevier
2025-03-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825000399 |
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| author | Lina M. Escobar Zita Bendahan Deisy Abril David Díaz-Baez Adolfo Paternina Farith González-Martinez Ma Clara González-Carrera |
| author_facet | Lina M. Escobar Zita Bendahan Deisy Abril David Díaz-Baez Adolfo Paternina Farith González-Martinez Ma Clara González-Carrera |
| author_sort | Lina M. Escobar |
| collection | DOAJ |
| description | The quantity and quality of DNA are crucial factors in determining polymorphisms in samples from individuals with and without pathologies such as cleft lip and palate (CL/P). Saliva is increasingly being evaluated as a collection method. This study aimed to compare saliva DNA concentrations from individuals of different ages with and without CL/P.Saliva samples were collected, DNA was extracted and the quantification was performed by spectrophotometry. Kruskal-Wallis test was used to compare frequencies between groups, and Spearman's rho for correlations.We obtained saliva DNA from 314 individuals: 107 with CL/P (mean age: 12.7 ± 6.5 years), 103 parents of children with CL/P (mean age: 41.1 ± 9.3 years), 52 individuals without CL/P under 18 years old (controls), and 52 individuals without CL/P over 18 years old (controls). The sample comprised 32.2 % females and 67.8 % males.Statistically significant differences in DNA concentrations were found between individuals under and over 18 years old (12.38 and 21.3 ng/μl, respectively, p = 0.0001), but no differences were observed between males and females. Individuals with CL/P had lower DNA concentrations (12.45 ng/μl) compared to their parents and controls (21.95 and 21.15 ng/μl, respectively). Our results showed a direct correlation between age and DNA concentration in individuals with CL/P under 18 years old (R = 0.328). |
| format | Article |
| id | doaj-art-6da419e1509a458c88911dd66a33750e |
| institution | Kabale University |
| issn | 2212-4268 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Oral Biology and Craniofacial Research |
| spelling | doaj-art-6da419e1509a458c88911dd66a33750e2025-02-11T04:34:51ZengElsevierJournal of Oral Biology and Craniofacial Research2212-42682025-03-01152246249Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNALina M. Escobar0Zita Bendahan1Deisy Abril2David Díaz-Baez3Adolfo Paternina4Farith González-Martinez5Ma Clara González-Carrera6Unidad de Manejo Integral de Malformaciones Craneofaciales (UMIMC), Facultad de Odontología, Universidad El Bosque, Bogotá, Colombia; Corresponding author.Unidad de Manejo Integral de Malformaciones Craneofaciales (UMIMC), Facultad de Odontología, Universidad El Bosque, Bogotá, ColombiaBacterial Molecular Genetics Laboratory (LGMB), Universidad El Bosque, Bogotá, ColombiaUnidad de Manejo Integral de Malformaciones Craneofaciales (UMIMC), Facultad de Odontología, Universidad El Bosque, Bogotá, ColombiaUnidad de Manejo Integral de Malformaciones Craneofaciales (UMIMC), Facultad de Odontología, Universidad El Bosque, Bogotá, Colombia; Grupo de Investigación en Salud Pública, Facultad de Odontología, Universidad de Cartagena, Cartagena de Indias, ColombiaGrupo de Investigación en Salud Pública, Facultad de Odontología, Universidad de Cartagena, Cartagena de Indias, ColombiaUnidad de Manejo Integral de Malformaciones Craneofaciales (UMIMC), Facultad de Odontología, Universidad El Bosque, Bogotá, ColombiaThe quantity and quality of DNA are crucial factors in determining polymorphisms in samples from individuals with and without pathologies such as cleft lip and palate (CL/P). Saliva is increasingly being evaluated as a collection method. This study aimed to compare saliva DNA concentrations from individuals of different ages with and without CL/P.Saliva samples were collected, DNA was extracted and the quantification was performed by spectrophotometry. Kruskal-Wallis test was used to compare frequencies between groups, and Spearman's rho for correlations.We obtained saliva DNA from 314 individuals: 107 with CL/P (mean age: 12.7 ± 6.5 years), 103 parents of children with CL/P (mean age: 41.1 ± 9.3 years), 52 individuals without CL/P under 18 years old (controls), and 52 individuals without CL/P over 18 years old (controls). The sample comprised 32.2 % females and 67.8 % males.Statistically significant differences in DNA concentrations were found between individuals under and over 18 years old (12.38 and 21.3 ng/μl, respectively, p = 0.0001), but no differences were observed between males and females. Individuals with CL/P had lower DNA concentrations (12.45 ng/μl) compared to their parents and controls (21.95 and 21.15 ng/μl, respectively). Our results showed a direct correlation between age and DNA concentration in individuals with CL/P under 18 years old (R = 0.328).http://www.sciencedirect.com/science/article/pii/S2212426825000399AgeCleft lip and palateDNASalivaGenetic polymorphism |
| spellingShingle | Lina M. Escobar Zita Bendahan Deisy Abril David Díaz-Baez Adolfo Paternina Farith González-Martinez Ma Clara González-Carrera Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA Journal of Oral Biology and Craniofacial Research Age Cleft lip and palate DNA Saliva Genetic polymorphism |
| title | Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA |
| title_full | Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA |
| title_fullStr | Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA |
| title_full_unstemmed | Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA |
| title_short | Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA |
| title_sort | influence of age sex and presence of cleft lip and palate on the quantity and quality of saliva derived dna |
| topic | Age Cleft lip and palate DNA Saliva Genetic polymorphism |
| url | http://www.sciencedirect.com/science/article/pii/S2212426825000399 |
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