Influence of age, sex, and presence of cleft lip and palate on the quantity and quality of saliva-derived DNA
The quantity and quality of DNA are crucial factors in determining polymorphisms in samples from individuals with and without pathologies such as cleft lip and palate (CL/P). Saliva is increasingly being evaluated as a collection method. This study aimed to compare saliva DNA concentrations from ind...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | Journal of Oral Biology and Craniofacial Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212426825000399 |
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| Summary: | The quantity and quality of DNA are crucial factors in determining polymorphisms in samples from individuals with and without pathologies such as cleft lip and palate (CL/P). Saliva is increasingly being evaluated as a collection method. This study aimed to compare saliva DNA concentrations from individuals of different ages with and without CL/P.Saliva samples were collected, DNA was extracted and the quantification was performed by spectrophotometry. Kruskal-Wallis test was used to compare frequencies between groups, and Spearman's rho for correlations.We obtained saliva DNA from 314 individuals: 107 with CL/P (mean age: 12.7 ± 6.5 years), 103 parents of children with CL/P (mean age: 41.1 ± 9.3 years), 52 individuals without CL/P under 18 years old (controls), and 52 individuals without CL/P over 18 years old (controls). The sample comprised 32.2 % females and 67.8 % males.Statistically significant differences in DNA concentrations were found between individuals under and over 18 years old (12.38 and 21.3 ng/μl, respectively, p = 0.0001), but no differences were observed between males and females. Individuals with CL/P had lower DNA concentrations (12.45 ng/μl) compared to their parents and controls (21.95 and 21.15 ng/μl, respectively). Our results showed a direct correlation between age and DNA concentration in individuals with CL/P under 18 years old (R = 0.328). |
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| ISSN: | 2212-4268 |