The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress
Drought conditions impact plants at the morphological, physiological, and molecular levels. Plant tolerance to drought conditions is frequently associated with maintaining proteome stability, highlighting the significance of proteomic analysis in understanding the mechanisms underlying plant resilie...
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MDPI AG
2025-07-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/14/14/2168 |
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| author | Regina Azarkina Arina Makeeva Anna Mamaeva Sergey Kovalchuk Daria Ganaeva Igor Tikhonovich Igor Fesenko |
| author_facet | Regina Azarkina Arina Makeeva Anna Mamaeva Sergey Kovalchuk Daria Ganaeva Igor Tikhonovich Igor Fesenko |
| author_sort | Regina Azarkina |
| collection | DOAJ |
| description | Drought conditions impact plants at the morphological, physiological, and molecular levels. Plant tolerance to drought conditions is frequently associated with maintaining proteome stability, highlighting the significance of proteomic analysis in understanding the mechanisms underlying plant resilience. Here, we performed proteomic and peptidomic analysis of spring wheat (<i>Triticum aestivum</i> L.) under drought stress conditions. Using isobaric tags for relative and absolute quantitation (iTRAQ), we identified 497 and 157 differentially abundant protein (DAP) groups in leaves and roots, respectively. The upregulated DAP groups in leaves were primarily involved in stress responses, such as oxidative stress and heat response, whereas those in roots were associated with responses to water deprivation and sulfur compound metabolic processes. The analysis of the extracellular root peptidome revealed 2294 native peptides, including members of small secreted peptide (SSP) families. In the peptidomes of stress-induced plants, we identified 16 SSPs as well as peptides derived from proteins involved in cell wall catabolism, intercellular signaling, and stress response. These peptides represent potential candidates as regulators of drought responses. Our results help us to understand adaptation mechanisms and develop new agricultural technologies to increase productivity. |
| format | Article |
| id | doaj-art-6da378f36f5e42b78891adb468eca80e |
| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-6da378f36f5e42b78891adb468eca80e2025-08-20T03:32:18ZengMDPI AGPlants2223-77472025-07-011414216810.3390/plants14142168The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought StressRegina Azarkina0Arina Makeeva1Anna Mamaeva2Sergey Kovalchuk3Daria Ganaeva4Igor Tikhonovich5Igor Fesenko6Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow 117997, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow 117997, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow 117997, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow 117997, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow 117997, RussiaAll-Russia Research Institute for Agricultural Microbiology, Podbelscogo hwy 3, Pushkin, St. Petersburg 196608, RussiaIndependent Researcher, Moscow 117997, RussiaDrought conditions impact plants at the morphological, physiological, and molecular levels. Plant tolerance to drought conditions is frequently associated with maintaining proteome stability, highlighting the significance of proteomic analysis in understanding the mechanisms underlying plant resilience. Here, we performed proteomic and peptidomic analysis of spring wheat (<i>Triticum aestivum</i> L.) under drought stress conditions. Using isobaric tags for relative and absolute quantitation (iTRAQ), we identified 497 and 157 differentially abundant protein (DAP) groups in leaves and roots, respectively. The upregulated DAP groups in leaves were primarily involved in stress responses, such as oxidative stress and heat response, whereas those in roots were associated with responses to water deprivation and sulfur compound metabolic processes. The analysis of the extracellular root peptidome revealed 2294 native peptides, including members of small secreted peptide (SSP) families. In the peptidomes of stress-induced plants, we identified 16 SSPs as well as peptides derived from proteins involved in cell wall catabolism, intercellular signaling, and stress response. These peptides represent potential candidates as regulators of drought responses. Our results help us to understand adaptation mechanisms and develop new agricultural technologies to increase productivity.https://www.mdpi.com/2223-7747/14/14/2168proteomepeptidomesecretomedroughtLC-MS/MSiTRAQ |
| spellingShingle | Regina Azarkina Arina Makeeva Anna Mamaeva Sergey Kovalchuk Daria Ganaeva Igor Tikhonovich Igor Fesenko The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress Plants proteome peptidome secretome drought LC-MS/MS iTRAQ |
| title | The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress |
| title_full | The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress |
| title_fullStr | The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress |
| title_full_unstemmed | The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress |
| title_short | The Proteomic and Peptidomic Response of Wheat (<i>Triticum aestivum</i> L.) to Drought Stress |
| title_sort | proteomic and peptidomic response of wheat i triticum aestivum i l to drought stress |
| topic | proteome peptidome secretome drought LC-MS/MS iTRAQ |
| url | https://www.mdpi.com/2223-7747/14/14/2168 |
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