Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss
Age-related hearing loss (ARHL) is an increasing and gradual sensorineural hearing dysfunction. Oxidative stress is an essential factor in developing ARHL; additionally, premature senescence of auditory cells induced by oxidative stress can produce hearing loss. Hydrogen peroxide (H<sub>2</...
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MDPI AG
2021-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/9/1497 |
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| author | Luz del Mar Rivas-Chacón Sofía Martínez-Rodríguez Raquel Madrid-García Joaquín Yanes-Díaz Juan Ignacio Riestra-Ayora Ricardo Sanz-Fernández Carolina Sánchez-Rodríguez |
| author_facet | Luz del Mar Rivas-Chacón Sofía Martínez-Rodríguez Raquel Madrid-García Joaquín Yanes-Díaz Juan Ignacio Riestra-Ayora Ricardo Sanz-Fernández Carolina Sánchez-Rodríguez |
| author_sort | Luz del Mar Rivas-Chacón |
| collection | DOAJ |
| description | Age-related hearing loss (ARHL) is an increasing and gradual sensorineural hearing dysfunction. Oxidative stress is an essential factor in developing ARHL; additionally, premature senescence of auditory cells induced by oxidative stress can produce hearing loss. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) represents a method commonly used to generate cellular senescence in vitro. The objective of the present paper is to study H<sub>2</sub>O<sub>2</sub>-induced senescence patterns in three auditory cell lines (House Ear Institute-Organ of Corti 1, HEI-OC1; organ of Corti, OC-k3, and stria vascularis, SV-k1 cells) to elucidate the intrinsic mechanisms responsible for ARHL. The auditory cells were exposed to H<sub>2</sub>O<sub>2</sub> at different concentrations and times. The results obtained show different responses of the hearing cells concerning cell growth, β-galactosidase activity, morphological changes, mitochondrial activation, levels of oxidative stress, and other markers of cell damage (Forkhead box O3a, FoxO3a, and 8-oxoguanine, 8-oxoG). Comparison between the responses of these auditory cells to H<sub>2</sub>O<sub>2</sub> is a helpful method to evaluate the molecular mechanisms responsible for these auditory cells’ senescence. Furthermore, this in vitro model could help develop anti-senescent therapeutic strategies for the treatment of AHRL. |
| format | Article |
| id | doaj-art-6d94fbcc7f4644e6bd90d554342d31c0 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-6d94fbcc7f4644e6bd90d554342d31c02025-01-03T16:53:02ZengMDPI AGAntioxidants2076-39212021-09-01109149710.3390/antiox10091497Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing LossLuz del Mar Rivas-Chacón0Sofía Martínez-Rodríguez1Raquel Madrid-García2Joaquín Yanes-Díaz3Juan Ignacio Riestra-Ayora4Ricardo Sanz-Fernández5Carolina Sánchez-Rodríguez6Department Clinical Analysis, Hospital Universitario de Getafe, Carretera de Toledo, km 12.500, Getafe, 28905 Madrid, SpainDepartment of Medicine, Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Villaviciosa de Odón, 28670 Madrid, SpainDepartment of Medicine, Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Villaviciosa de Odón, 28670 Madrid, SpainDepartment Otolaryngology, Hospital Universitario de Getafe, Carretera de Toledo, km 12.500, Getafe, 28905 Madrid, SpainDepartment of Medicine, Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Villaviciosa de Odón, 28670 Madrid, SpainDepartment Otolaryngology, Hospital Universitario de Getafe, Carretera de Toledo, km 12.500, Getafe, 28905 Madrid, SpainDepartment of Medicine, Faculty of Biomedical and Health Sciences, Universidad Europea de Madrid, Villaviciosa de Odón, 28670 Madrid, SpainAge-related hearing loss (ARHL) is an increasing and gradual sensorineural hearing dysfunction. Oxidative stress is an essential factor in developing ARHL; additionally, premature senescence of auditory cells induced by oxidative stress can produce hearing loss. Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) represents a method commonly used to generate cellular senescence in vitro. The objective of the present paper is to study H<sub>2</sub>O<sub>2</sub>-induced senescence patterns in three auditory cell lines (House Ear Institute-Organ of Corti 1, HEI-OC1; organ of Corti, OC-k3, and stria vascularis, SV-k1 cells) to elucidate the intrinsic mechanisms responsible for ARHL. The auditory cells were exposed to H<sub>2</sub>O<sub>2</sub> at different concentrations and times. The results obtained show different responses of the hearing cells concerning cell growth, β-galactosidase activity, morphological changes, mitochondrial activation, levels of oxidative stress, and other markers of cell damage (Forkhead box O3a, FoxO3a, and 8-oxoguanine, 8-oxoG). Comparison between the responses of these auditory cells to H<sub>2</sub>O<sub>2</sub> is a helpful method to evaluate the molecular mechanisms responsible for these auditory cells’ senescence. Furthermore, this in vitro model could help develop anti-senescent therapeutic strategies for the treatment of AHRL.https://www.mdpi.com/2076-3921/10/9/1497age-related hearing losssenescenceauditory cellhydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)oxidative stress |
| spellingShingle | Luz del Mar Rivas-Chacón Sofía Martínez-Rodríguez Raquel Madrid-García Joaquín Yanes-Díaz Juan Ignacio Riestra-Ayora Ricardo Sanz-Fernández Carolina Sánchez-Rodríguez Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss Antioxidants age-related hearing loss senescence auditory cell hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) oxidative stress |
| title | Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss |
| title_full | Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss |
| title_fullStr | Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss |
| title_full_unstemmed | Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss |
| title_short | Role of Oxidative Stress in the Senescence Pattern of Auditory Cells in Age-Related Hearing Loss |
| title_sort | role of oxidative stress in the senescence pattern of auditory cells in age related hearing loss |
| topic | age-related hearing loss senescence auditory cell hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) oxidative stress |
| url | https://www.mdpi.com/2076-3921/10/9/1497 |
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