The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM)
Nontuberculous mycobacteria (NTM) are environmental microorganisms, also known as opportunistic pathogens, found in patients with pulmonary tuberculosis. The emergence of antibiotic resistance is increased by prolonged antibiotic treatment for NTM infections. Therefore, alternative sources of new an...
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Kementerian Kelautan dan Perikanan
2024-12-01
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| Series: | Squalen |
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| Online Access: | https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/882 |
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| author | Ni Luh Putu Vidya Paramita Akhirta Atikana Linda Sukmarini Febriana Untari Muhammad Bagas Prayoga Faiqoh Nur Maulidia Shanti Ratnakomala Puspita Lisdiyanti Titik Nuryastuti Triana Hertiani |
| author_facet | Ni Luh Putu Vidya Paramita Akhirta Atikana Linda Sukmarini Febriana Untari Muhammad Bagas Prayoga Faiqoh Nur Maulidia Shanti Ratnakomala Puspita Lisdiyanti Titik Nuryastuti Triana Hertiani |
| author_sort | Ni Luh Putu Vidya Paramita |
| collection | DOAJ |
| description | Nontuberculous mycobacteria (NTM) are environmental microorganisms, also known as opportunistic pathogens, found in patients with pulmonary tuberculosis. The emergence of antibiotic resistance is increased by prolonged antibiotic treatment for NTM infections. Therefore, alternative sources of new antibiotics are essential for the treatment of NTM infections. A marine actinobacterium, Streptomyces sp. BTA 1-131, isolated from a marine sponge, Melophlus sarasinorum, has been reported as a potential source of antibacterials and anticancer agents. The present study aimed to investigate the potential of Streptomyces sp. BTA 1-131 against two NTMs: Mycobacterium smegmatis and Mycobacterium fortuitum. Streptomyces sp. BTA 1-131 was fermented in three cultivation media (SYP, ISP2, and YS), and the secondary metabolites were extracted using methanol. The bioactivity screening showed inhibition of all methanolic extracts against the growth of M. smegmatis and M. fortuitum. The methanolic extract, which could inhibit both mycobacteria, was a crude extract derived from SYP liquid medium fermentation. The isolated compounds in this study were preliminarily identified using thin-layer chromatography (TLC). The TLC results showed different potential compounds in the crude extracts of Streptomyces sp. BTA 1-131 also highlighted the impact of the fermentation medium on the production of metabolites from Streptomyces. This study also added knowledge about the importance of the Indonesian marine actinobacterium Streptomyces sp. BTA 1-131 as a promising producer of anti-NTM compounds. |
| format | Article |
| id | doaj-art-6d843bf395424f0f95067bddd90b5846 |
| institution | Kabale University |
| issn | 2089-5690 2406-9272 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Kementerian Kelautan dan Perikanan |
| record_format | Article |
| series | Squalen |
| spelling | doaj-art-6d843bf395424f0f95067bddd90b58462024-12-31T14:51:18ZengKementerian Kelautan dan PerikananSqualen2089-56902406-92722024-12-0119310.15578/squalen.882379The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM)Ni Luh Putu Vidya Paramita0Akhirta Atikana1Linda Sukmarini2Febriana Untari3Muhammad Bagas Prayoga4Faiqoh Nur Maulidia5Shanti Ratnakomala6Puspita Lisdiyanti7Titik Nuryastuti8Triana Hertiani9Udayana University. Doctoral Program in Pharmaceutical Science, Faculty of Pharmacy, Universitas Gadjah Mada.National Research and Innovation Agency (BRIN). Indonesian Biofilm Research Collaboration Centre.National Research and Innovation Agency (BRIN). Indonesian Biofilm Research Collaboration Centre.National Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN)National Research and Innovation Agency (BRIN)National Research and Innovation Agency. Indonesian Biofilm Research Collaboration Centre.National Research and Innovation Agency (BRIN). Indonesian Biofilm Research Collaboration Centre.Universitas Gadjah Mada. Indonesian Biofilm Research Collaboration Centre.Universitas Gadjah Mada. Indonesian Biofilm Research Collaboration Centre.Nontuberculous mycobacteria (NTM) are environmental microorganisms, also known as opportunistic pathogens, found in patients with pulmonary tuberculosis. The emergence of antibiotic resistance is increased by prolonged antibiotic treatment for NTM infections. Therefore, alternative sources of new antibiotics are essential for the treatment of NTM infections. A marine actinobacterium, Streptomyces sp. BTA 1-131, isolated from a marine sponge, Melophlus sarasinorum, has been reported as a potential source of antibacterials and anticancer agents. The present study aimed to investigate the potential of Streptomyces sp. BTA 1-131 against two NTMs: Mycobacterium smegmatis and Mycobacterium fortuitum. Streptomyces sp. BTA 1-131 was fermented in three cultivation media (SYP, ISP2, and YS), and the secondary metabolites were extracted using methanol. The bioactivity screening showed inhibition of all methanolic extracts against the growth of M. smegmatis and M. fortuitum. The methanolic extract, which could inhibit both mycobacteria, was a crude extract derived from SYP liquid medium fermentation. The isolated compounds in this study were preliminarily identified using thin-layer chromatography (TLC). The TLC results showed different potential compounds in the crude extracts of Streptomyces sp. BTA 1-131 also highlighted the impact of the fermentation medium on the production of metabolites from Streptomyces. This study also added knowledge about the importance of the Indonesian marine actinobacterium Streptomyces sp. BTA 1-131 as a promising producer of anti-NTM compounds.https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/882marinenontuberculous mycobacteriaantimycobacterialantibiotic |
| spellingShingle | Ni Luh Putu Vidya Paramita Akhirta Atikana Linda Sukmarini Febriana Untari Muhammad Bagas Prayoga Faiqoh Nur Maulidia Shanti Ratnakomala Puspita Lisdiyanti Titik Nuryastuti Triana Hertiani The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM) Squalen marine nontuberculous mycobacteria antimycobacterial antibiotic |
| title | The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM) |
| title_full | The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM) |
| title_fullStr | The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM) |
| title_full_unstemmed | The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM) |
| title_short | The Marine Actinobacterium Streptomyces sp. BTA 1-131 as a Potential Producer of Anti-Nontuberculous Mycobacterial (Anti-NTM) |
| title_sort | marine actinobacterium streptomyces sp bta 1 131 as a potential producer of anti nontuberculous mycobacterial anti ntm |
| topic | marine nontuberculous mycobacteria antimycobacterial antibiotic |
| url | https://www.bbp4b.litbang.kkp.go.id/squalen-bulletin/index.php/squalen/article/view/882 |
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