Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activity

Graphene oxide (GO) has been unlocked as a potential bactericidal agent with multifaceted applications for a high degree of abundance of oxidizing functional groups over its structure. The potassium doping of GO (K-GO) enhances its biocompatibility and antimicrobial efficacy. Herein we present a one...

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Main Authors: Kamal Garwal, Chetna Tewari, Tanuja Arya, Jyoti Rawat, Veena Pande, Souvik Basak, Mayukh Bose, Yong Chae Jung, Nanda Gopal Sahoo
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Plant Nano Biology
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Online Access:http://www.sciencedirect.com/science/article/pii/S2773111124000627
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author Kamal Garwal
Chetna Tewari
Tanuja Arya
Jyoti Rawat
Veena Pande
Souvik Basak
Mayukh Bose
Yong Chae Jung
Nanda Gopal Sahoo
author_facet Kamal Garwal
Chetna Tewari
Tanuja Arya
Jyoti Rawat
Veena Pande
Souvik Basak
Mayukh Bose
Yong Chae Jung
Nanda Gopal Sahoo
author_sort Kamal Garwal
collection DOAJ
description Graphene oxide (GO) has been unlocked as a potential bactericidal agent with multifaceted applications for a high degree of abundance of oxidizing functional groups over its structure. The potassium doping of GO (K-GO) enhances its biocompatibility and antimicrobial efficacy. Herein we present a one-step synthesis of K-GO using Quercus ilex (oak fruit) as a sustainable precursor. Characterization via Raman spectroscopy, UV-Vis spectroscopy, TEM, FT-IR, and TGA confirmed the successful synthesis of K-GO. The screening of antimicrobial activity of K-GO was undertaken against Gram-positive (E. faecalis and S. aureus), Gram-negative bacteria (E. coli and P. aeruginosa), and selected fungus (C. albicans and T. asperellum) using the disc diffusion assay. The midpoint inhibitory concentration (IC50) of K-GO against E. coli and S. aureus was found to be 109.64 µg/mL and 38.90 µg/mL, respectively. Employing our green synthesis method using oak seeds as a precursor showcases both cost-effectiveness and sustainability. The aforementioned results suggest that K-GO nano-sheet possesses significant antimicrobial activity, thus may evolve as future antimicrobial nanomaterials.
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spelling doaj-art-6d7dd6356f9042c9abcad6090fabc3b12025-08-20T02:49:05ZengElsevierPlant Nano Biology2773-11112024-11-011010011910.1016/j.plana.2024.100119Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activityKamal Garwal0Chetna Tewari1Tanuja Arya2Jyoti Rawat3Veena Pande4Souvik Basak5Mayukh Bose6Yong Chae Jung7Nanda Gopal Sahoo8Prof. Rajendra Singh Nanoscience and Nanotechnology Centre, Department of Chemistry, D.S.B. Campus, Kumaun University, Nainital, Uttarakhand 263001, IndiaRAMP Convergence Research Center, Korea Institute of Science and Technology (KIST), 92 Chudong-Ro, Bongdong-Eup, Wanju-Gun, Jeonbuk, 55324, Republic of KoreaProf. Rajendra Singh Nanoscience and Nanotechnology Centre, Department of Chemistry, D.S.B. Campus, Kumaun University, Nainital, Uttarakhand 263001, IndiaDepartment of Biotechnology, Sir J. C. Bose Technical Campus, Kumaun University, Bhimtal, Nainital, Uttarakhand 263136, IndiaDepartment of Biotechnology, Sir J. C. Bose Technical Campus, Kumaun University, Bhimtal, Nainital, Uttarakhand 263136, IndiaDr. B.C. Roy College of Pharmacy & Allied Health Sciences, Durgapur, West Bengal, IndiaBose Informatics, Kolkata, IndiaRAMP Convergence Research Center, Korea Institute of Science and Technology (KIST), 92 Chudong-Ro, Bongdong-Eup, Wanju-Gun, Jeonbuk, 55324, Republic of Korea; Corresponding authors.Prof. Rajendra Singh Nanoscience and Nanotechnology Centre, Department of Chemistry, D.S.B. Campus, Kumaun University, Nainital, Uttarakhand 263001, India; Corresponding authors.Graphene oxide (GO) has been unlocked as a potential bactericidal agent with multifaceted applications for a high degree of abundance of oxidizing functional groups over its structure. The potassium doping of GO (K-GO) enhances its biocompatibility and antimicrobial efficacy. Herein we present a one-step synthesis of K-GO using Quercus ilex (oak fruit) as a sustainable precursor. Characterization via Raman spectroscopy, UV-Vis spectroscopy, TEM, FT-IR, and TGA confirmed the successful synthesis of K-GO. The screening of antimicrobial activity of K-GO was undertaken against Gram-positive (E. faecalis and S. aureus), Gram-negative bacteria (E. coli and P. aeruginosa), and selected fungus (C. albicans and T. asperellum) using the disc diffusion assay. The midpoint inhibitory concentration (IC50) of K-GO against E. coli and S. aureus was found to be 109.64 µg/mL and 38.90 µg/mL, respectively. Employing our green synthesis method using oak seeds as a precursor showcases both cost-effectiveness and sustainability. The aforementioned results suggest that K-GO nano-sheet possesses significant antimicrobial activity, thus may evolve as future antimicrobial nanomaterials.http://www.sciencedirect.com/science/article/pii/S2773111124000627Agricultural wasteQuercus ilexPotassium-doped graphene oxideGreen synthesisAntibacterial microbial
spellingShingle Kamal Garwal
Chetna Tewari
Tanuja Arya
Jyoti Rawat
Veena Pande
Souvik Basak
Mayukh Bose
Yong Chae Jung
Nanda Gopal Sahoo
Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activity
Plant Nano Biology
Agricultural waste
Quercus ilex
Potassium-doped graphene oxide
Green synthesis
Antibacterial microbial
title Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activity
title_full Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activity
title_fullStr Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activity
title_full_unstemmed Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activity
title_short Green synthesis of agro-waste–derived potassium-doped graphene oxide for antimicrobial activity
title_sort green synthesis of agro waste derived potassium doped graphene oxide for antimicrobial activity
topic Agricultural waste
Quercus ilex
Potassium-doped graphene oxide
Green synthesis
Antibacterial microbial
url http://www.sciencedirect.com/science/article/pii/S2773111124000627
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