Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study
Introduction: Learning and memory allow individuals to adapt to their environmental needs and survive. Fish have the ability to solve complex learning tasks, associative learning, and flexible spatial memory. The proto-oncogene fos (c-fos) has been reported to be involved in brain development, learn...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-01-01
|
| Series: | Frontiers in Cell and Developmental Biology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2025.1503066/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832584997433769984 |
|---|---|
| author | Qiuling Wang Qiuling Wang Qiuling Wang Lixin Zhang Lixin Zhang Lixin Zhang Chenyuan Zhu Chenyuan Zhu Chenyuan Zhu Ke Lu Ke Lu Ke Lu Jiaqi Wu Jiaqi Wu Jiaqi Wu Xu-Fang Liang Xu-Fang Liang Xu-Fang Liang |
| author_facet | Qiuling Wang Qiuling Wang Qiuling Wang Lixin Zhang Lixin Zhang Lixin Zhang Chenyuan Zhu Chenyuan Zhu Chenyuan Zhu Ke Lu Ke Lu Ke Lu Jiaqi Wu Jiaqi Wu Jiaqi Wu Xu-Fang Liang Xu-Fang Liang Xu-Fang Liang |
| author_sort | Qiuling Wang |
| collection | DOAJ |
| description | Introduction: Learning and memory allow individuals to adapt to their environmental needs and survive. Fish have the ability to solve complex learning tasks, associative learning, and flexible spatial memory. The proto-oncogene fos (c-fos) has been reported to be involved in brain development, learning and memory in mammals. However, whether the c-fos plays a vital role in learning and memory in fish is unclear.Methods: Almost all fish have two paralogues of c-fos named fosaa and fosab. We used CRISPR/Cas9 technology to generate fosaa and fosab knockout zebrafish models.Results: In this study, we discovered the brain weight marked reduction in fosaa−/− and fosab−/− zebrafish compared with the wild-type (WT) (AB strain) zebrafish. In the T-maze behavioral assay, the fosab−/− zebrafish took significantly more than the average time to complete the assigned trial as the increase in the days compared to WT zebrafish, while the fosaa−/− zebrafish did not show a notable variance. The average time to complete the trial in fosab−/− zebrafish was significantly higher than in WT zebrafish. The relative mRNA expression level of c-jun in fosab−/− zebrafish was significantly higher than that in WT zebrafish, while the fosaa−/− zebrafish has no discernible trend. Additionally, the phylogenetic and multiple amino acid alignment results indicated that fish fosab has a higher identity with mammals Fos.Discussion: By integrating the above results, we found that fosab, but not fosaa, may possess a learning and memory function in fish. For the first time, we illustrated the role of fosaa and fosab in learning and memory via c-fos knockout in fish, which can provide new insights into environmental adaptation. |
| format | Article |
| id | doaj-art-6d77cb73d4934f3dbf7a4d7d01134ef7 |
| institution | Kabale University |
| issn | 2296-634X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj-art-6d77cb73d4934f3dbf7a4d7d01134ef72025-01-27T06:40:08ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2025-01-011310.3389/fcell.2025.15030661503066Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout studyQiuling Wang0Qiuling Wang1Qiuling Wang2Lixin Zhang3Lixin Zhang4Lixin Zhang5Chenyuan Zhu6Chenyuan Zhu7Chenyuan Zhu8Ke Lu9Ke Lu10Ke Lu11Jiaqi Wu12Jiaqi Wu13Jiaqi Wu14Xu-Fang Liang15Xu-Fang Liang16Xu-Fang Liang17College of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, ChinaEngineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, ChinaHubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, ChinaEngineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, ChinaHubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, ChinaEngineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, ChinaHubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, ChinaEngineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, ChinaHubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, ChinaEngineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, ChinaHubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan, ChinaCollege of Fisheries, Huazhong Agricultural University, Wuhan, Hubei, ChinaEngineering Research Center of Green development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, ChinaHubei Hongshan Laboratory, Fishery College, Huazhong Agricultural University, Wuhan, ChinaIntroduction: Learning and memory allow individuals to adapt to their environmental needs and survive. Fish have the ability to solve complex learning tasks, associative learning, and flexible spatial memory. The proto-oncogene fos (c-fos) has been reported to be involved in brain development, learning and memory in mammals. However, whether the c-fos plays a vital role in learning and memory in fish is unclear.Methods: Almost all fish have two paralogues of c-fos named fosaa and fosab. We used CRISPR/Cas9 technology to generate fosaa and fosab knockout zebrafish models.Results: In this study, we discovered the brain weight marked reduction in fosaa−/− and fosab−/− zebrafish compared with the wild-type (WT) (AB strain) zebrafish. In the T-maze behavioral assay, the fosab−/− zebrafish took significantly more than the average time to complete the assigned trial as the increase in the days compared to WT zebrafish, while the fosaa−/− zebrafish did not show a notable variance. The average time to complete the trial in fosab−/− zebrafish was significantly higher than in WT zebrafish. The relative mRNA expression level of c-jun in fosab−/− zebrafish was significantly higher than that in WT zebrafish, while the fosaa−/− zebrafish has no discernible trend. Additionally, the phylogenetic and multiple amino acid alignment results indicated that fish fosab has a higher identity with mammals Fos.Discussion: By integrating the above results, we found that fosab, but not fosaa, may possess a learning and memory function in fish. For the first time, we illustrated the role of fosaa and fosab in learning and memory via c-fos knockout in fish, which can provide new insights into environmental adaptation.https://www.frontiersin.org/articles/10.3389/fcell.2025.1503066/fullfosaafosablearning and memorybrain developmentzebrafish |
| spellingShingle | Qiuling Wang Qiuling Wang Qiuling Wang Lixin Zhang Lixin Zhang Lixin Zhang Chenyuan Zhu Chenyuan Zhu Chenyuan Zhu Ke Lu Ke Lu Ke Lu Jiaqi Wu Jiaqi Wu Jiaqi Wu Xu-Fang Liang Xu-Fang Liang Xu-Fang Liang Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study Frontiers in Cell and Developmental Biology fosaa fosab learning and memory brain development zebrafish |
| title | Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study |
| title_full | Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study |
| title_fullStr | Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study |
| title_full_unstemmed | Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study |
| title_short | Fosab, but not fosaa, plays important role in learning and memory in fish—insights from zebrafish gene knockout study |
| title_sort | fosab but not fosaa plays important role in learning and memory in fish insights from zebrafish gene knockout study |
| topic | fosaa fosab learning and memory brain development zebrafish |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2025.1503066/full |
| work_keys_str_mv | AT qiulingwang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT qiulingwang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT qiulingwang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT lixinzhang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT lixinzhang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT lixinzhang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT chenyuanzhu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT chenyuanzhu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT chenyuanzhu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT kelu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT kelu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT kelu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT jiaqiwu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT jiaqiwu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT jiaqiwu fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT xufangliang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT xufangliang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy AT xufangliang fosabbutnotfosaaplaysimportantroleinlearningandmemoryinfishinsightsfromzebrafishgeneknockoutstudy |