Opposite roles of serotonin and neuropeptide FMRFamide in the regulation of epigenetic processes involved in the longterm memory formation
Epigenetic modifications are studied intensively to understand mechanisms of long-term memory. We have shown that histone H3 methylation is important for the defense reflexes formation in the mollusk Helix lucorum. We suggested that these epigenetic modifications are controlled by facilitatory and i...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2016-05-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/596 |
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| author | L. N. Grinkevich O. V. Vorobiova |
| author_facet | L. N. Grinkevich O. V. Vorobiova |
| author_sort | L. N. Grinkevich |
| collection | DOAJ |
| description | Epigenetic modifications are studied intensively to understand mechanisms of long-term memory. We have shown that histone H3 methylation is important for the defense reflexes formation in the mollusk Helix lucorum. We suggested that these epigenetic modifications are controlled by facilitatory and inhibitory pathways involved in the long-term memory formation. Serotonin and neuropeptide FMRFamide play opposite roles in the formation of defensive reflexes. Serotonin strengthens synaptic connections between neurons of the network, and FMRFamid is an inhibitory transmitter leading to long-term depression. To study the epigenetic regulation of the processes involved in the long-term memory formation, we performed comparative studies on the serotonin and FMRFamide effects on histone H3 methylation in the CNS of the Helix. We found that the incubation of the CNS with serotonin induces methylation of histone H3 at both activating (Н3K4me3) and inhibitory (Н3K9me2) sites, while incubation with FMRFamide has an opposite effect reducing methylation of histone H3 in the subesophageal complex of ganglia, important for defensive behaviour. We observed a different methylation pattern of histone H3 in the cerebral ganglia involved in signal processing of food stimuli, where serotonin did not affect the methylation of histone H3 at the activator site and reduced methylation at the inhibitory site, while FMRFamid had no effect on methylation. The data indicate that the facilitatory and inhibitory processes mediated by serotonin and FMRFamide can interact at the epigenetic level, through histone H3 methylation by activating or inhibiting it, respectively. This may underlie the convergence of the activator and inhibitory pathways involved in the long-term memory formation and underlie following regulation of the expression of genes involved in long-term plasticity. |
| format | Article |
| id | doaj-art-6d733b8d297a473cab17345cbfad9948 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2016-05-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-6d733b8d297a473cab17345cbfad99482025-02-01T09:58:03ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592016-05-0120226226810.18699/VJ16.128475Opposite roles of serotonin and neuropeptide FMRFamide in the regulation of epigenetic processes involved in the longterm memory formationL. N. Grinkevich0O. V. Vorobiova1Pavlov Institute of Physiology RAS, St. Petersburg, RussiaPavlov Institute of Physiology RAS, St. Petersburg, RussiaEpigenetic modifications are studied intensively to understand mechanisms of long-term memory. We have shown that histone H3 methylation is important for the defense reflexes formation in the mollusk Helix lucorum. We suggested that these epigenetic modifications are controlled by facilitatory and inhibitory pathways involved in the long-term memory formation. Serotonin and neuropeptide FMRFamide play opposite roles in the formation of defensive reflexes. Serotonin strengthens synaptic connections between neurons of the network, and FMRFamid is an inhibitory transmitter leading to long-term depression. To study the epigenetic regulation of the processes involved in the long-term memory formation, we performed comparative studies on the serotonin and FMRFamide effects on histone H3 methylation in the CNS of the Helix. We found that the incubation of the CNS with serotonin induces methylation of histone H3 at both activating (Н3K4me3) and inhibitory (Н3K9me2) sites, while incubation with FMRFamide has an opposite effect reducing methylation of histone H3 in the subesophageal complex of ganglia, important for defensive behaviour. We observed a different methylation pattern of histone H3 in the cerebral ganglia involved in signal processing of food stimuli, where serotonin did not affect the methylation of histone H3 at the activator site and reduced methylation at the inhibitory site, while FMRFamid had no effect on methylation. The data indicate that the facilitatory and inhibitory processes mediated by serotonin and FMRFamide can interact at the epigenetic level, through histone H3 methylation by activating or inhibiting it, respectively. This may underlie the convergence of the activator and inhibitory pathways involved in the long-term memory formation and underlie following regulation of the expression of genes involved in long-term plasticity.https://vavilov.elpub.ru/jour/article/view/596epigeneticshistone h3 methylationserotoninfmrfamidmemory formationinhibitionmollusk helix |
| spellingShingle | L. N. Grinkevich O. V. Vorobiova Opposite roles of serotonin and neuropeptide FMRFamide in the regulation of epigenetic processes involved in the longterm memory formation Вавиловский журнал генетики и селекции epigenetics histone h3 methylation serotonin fmrfamid memory formation inhibition mollusk helix |
| title | Opposite roles of serotonin and neuropeptide FMRFamide in the regulation of epigenetic processes involved in the longterm memory formation |
| title_full | Opposite roles of serotonin and neuropeptide FMRFamide in the regulation of epigenetic processes involved in the longterm memory formation |
| title_fullStr | Opposite roles of serotonin and neuropeptide FMRFamide in the regulation of epigenetic processes involved in the longterm memory formation |
| title_full_unstemmed | Opposite roles of serotonin and neuropeptide FMRFamide in the regulation of epigenetic processes involved in the longterm memory formation |
| title_short | Opposite roles of serotonin and neuropeptide FMRFamide in the regulation of epigenetic processes involved in the longterm memory formation |
| title_sort | opposite roles of serotonin and neuropeptide fmrfamide in the regulation of epigenetic processes involved in the longterm memory formation |
| topic | epigenetics histone h3 methylation serotonin fmrfamid memory formation inhibition mollusk helix |
| url | https://vavilov.elpub.ru/jour/article/view/596 |
| work_keys_str_mv | AT lngrinkevich oppositerolesofserotoninandneuropeptidefmrfamideintheregulationofepigeneticprocessesinvolvedinthelongtermmemoryformation AT ovvorobiova oppositerolesofserotoninandneuropeptidefmrfamideintheregulationofepigeneticprocessesinvolvedinthelongtermmemoryformation |