Preventive Gastroprotective Effect of a Functional Food Based on Quinoa (<i>Chenopodium quinoa</i> Willd.) and Quercetin in a Murine Model of Ibuprofen-Induced Gastric Damage
Nonsteroidal anti-inflammatory drug-based therapies are the cause of 20–30% cases of gastric lesions in chronic users worldwide. Co-medication with omeprazole (OMP) is the most commonly used option to prevent these lesions, although this carries risks of its own; thus, alternatives are being explore...
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2025-07-01
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| author | Maribel Valenzuela-González José Luis Cárdenas-López Armando Burgos-Hernández Norma Julieta Salazar-López Manuel Viuda-Martos Mónica A. Villegas-Ochoa Gustavo Martínez-Coronilla J. Abraham Domínguez-Avila Shela Gorinstein Gustavo A. González-Aguilar Rosario Maribel Robles-Sánchez |
| author_facet | Maribel Valenzuela-González José Luis Cárdenas-López Armando Burgos-Hernández Norma Julieta Salazar-López Manuel Viuda-Martos Mónica A. Villegas-Ochoa Gustavo Martínez-Coronilla J. Abraham Domínguez-Avila Shela Gorinstein Gustavo A. González-Aguilar Rosario Maribel Robles-Sánchez |
| author_sort | Maribel Valenzuela-González |
| collection | DOAJ |
| description | Nonsteroidal anti-inflammatory drug-based therapies are the cause of 20–30% cases of gastric lesions in chronic users worldwide. Co-medication with omeprazole (OMP) is the most commonly used option to prevent these lesions, although this carries risks of its own; thus, alternatives are being explored, such as dietary antioxidant therapies. The objective of this study was to evaluate the gastroprotective activity of quinoa (<i>Chenopodium quinoa</i> Willd.) on ibuprofen (IBP)-induced gastric ulcers in a rat model. Quinoa cookies were formulated with heat-treated quinoa using microwave radiation. The intestinal bioaccessibility of phenols and flavonoids, and the antioxidant activity of microwaved quinoa cookies (MQCs) were notably higher than quinoa cookies without thermal treatment (RQCs): 132% TPC, 52% TFC, 1564% TEAC vs. 67% TPC, 24% TFC, and 958% TEAC, respectively. Basal diets were supplemented with MQCs (20%) and quercetin (Q, 0.20%) as a reference flavonoid and administered for 30 days. Gastric lesions were induced by intragastric IBP doses, with OMP treatment as a positive control. Gastric damage index (macroscopic study), histological score (microscopic study), and plasma antioxidant enzyme activity (SOD and CAT) were evaluated. Macroscopic results showed that the addition of MQCs, Q, and OMP decreased the gastric damage index (GDI) by 50%, 40%, and 3%, respectively, as compared to IBP (GDI 100%). Histological analyses showed neutrophil infiltration and congested blood vessels in IBP-treated tissues; in contrast, the experimental diet groups showed lower infiltration for MQC > OMP > Q, respectively. A significant increase in SOD and CAT enzyme activity was observed in the MQC and Q groups as compared to the IBP group. We conclude that a reduction in the GDI and histological score was observed in IBP-induced murine models fed diets containing 20% MQC and 0.20% Q, demonstrating a preventive gastroprotective effect. |
| format | Article |
| id | doaj-art-6d6ceed459ed418dba1c3b76cf79b206 |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-07-01 |
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| series | Antioxidants |
| spelling | doaj-art-6d6ceed459ed418dba1c3b76cf79b2062025-08-20T03:58:26ZengMDPI AGAntioxidants2076-39212025-07-0114789310.3390/antiox14070893Preventive Gastroprotective Effect of a Functional Food Based on Quinoa (<i>Chenopodium quinoa</i> Willd.) and Quercetin in a Murine Model of Ibuprofen-Induced Gastric DamageMaribel Valenzuela-González0José Luis Cárdenas-López1Armando Burgos-Hernández2Norma Julieta Salazar-López3Manuel Viuda-Martos4Mónica A. Villegas-Ochoa5Gustavo Martínez-Coronilla6J. Abraham Domínguez-Avila7Shela Gorinstein8Gustavo A. González-Aguilar9Rosario Maribel Robles-Sánchez10Departamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales Col. Centro. A.P., Hermosillo 83000, Sonora, MexicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales Col. Centro. A.P., Hermosillo 83000, Sonora, MexicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales Col. Centro. A.P., Hermosillo 83000, Sonora, MexicoFacultad de Medicina de Mexicali, Universidad Autónoma de Baja California, Dr. Humberto Torres Sanginés S/N, Centro Cívico, Mexicali 21000, Baja California, MexicoIPOA Research Group, Centro de Investigación e Innovación Agroalimentaria y Agroambiental, Universidad Miguel Hernández (CIAGRO-UMH), Ctra. Beniel Km 3.1, 03312 Orihuela, SpainCentro de Investigación en Alimentación y Desarrollo, A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Colonia La Victoria, Hermosillo 83304, Sonora, MexicoFacultad de Medicina de Mexicali, Universidad Autónoma de Baja California, Dr. Humberto Torres Sanginés S/N, Centro Cívico, Mexicali 21000, Baja California, MexicoSECIHTI-Centro de Investigación en Alimentación y Desarrollo, A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Colonia La Victoria, Hermosillo 83304, Sonora, MexicoDepartment of Medicinal Chemistry and Natural Products, School of Pharmacy, The Hebrew University, Hadassah Medical School, Jerusalem 91120, IsraelCentro de Investigación en Alimentación y Desarrollo, A.C., Carretera Gustavo Enrique Astiazarán Rosas, No. 46, Colonia La Victoria, Hermosillo 83304, Sonora, MexicoDepartamento de Investigación y Posgrado en Alimentos, Universidad de Sonora, Blvd. Luis Encinas y Rosales Col. Centro. A.P., Hermosillo 83000, Sonora, MexicoNonsteroidal anti-inflammatory drug-based therapies are the cause of 20–30% cases of gastric lesions in chronic users worldwide. Co-medication with omeprazole (OMP) is the most commonly used option to prevent these lesions, although this carries risks of its own; thus, alternatives are being explored, such as dietary antioxidant therapies. The objective of this study was to evaluate the gastroprotective activity of quinoa (<i>Chenopodium quinoa</i> Willd.) on ibuprofen (IBP)-induced gastric ulcers in a rat model. Quinoa cookies were formulated with heat-treated quinoa using microwave radiation. The intestinal bioaccessibility of phenols and flavonoids, and the antioxidant activity of microwaved quinoa cookies (MQCs) were notably higher than quinoa cookies without thermal treatment (RQCs): 132% TPC, 52% TFC, 1564% TEAC vs. 67% TPC, 24% TFC, and 958% TEAC, respectively. Basal diets were supplemented with MQCs (20%) and quercetin (Q, 0.20%) as a reference flavonoid and administered for 30 days. Gastric lesions were induced by intragastric IBP doses, with OMP treatment as a positive control. Gastric damage index (macroscopic study), histological score (microscopic study), and plasma antioxidant enzyme activity (SOD and CAT) were evaluated. Macroscopic results showed that the addition of MQCs, Q, and OMP decreased the gastric damage index (GDI) by 50%, 40%, and 3%, respectively, as compared to IBP (GDI 100%). Histological analyses showed neutrophil infiltration and congested blood vessels in IBP-treated tissues; in contrast, the experimental diet groups showed lower infiltration for MQC > OMP > Q, respectively. A significant increase in SOD and CAT enzyme activity was observed in the MQC and Q groups as compared to the IBP group. We conclude that a reduction in the GDI and histological score was observed in IBP-induced murine models fed diets containing 20% MQC and 0.20% Q, demonstrating a preventive gastroprotective effect.https://www.mdpi.com/2076-3921/14/7/893oxidative stressgastric lesionpseudocerealsgastroprotectionflavonoids |
| spellingShingle | Maribel Valenzuela-González José Luis Cárdenas-López Armando Burgos-Hernández Norma Julieta Salazar-López Manuel Viuda-Martos Mónica A. Villegas-Ochoa Gustavo Martínez-Coronilla J. Abraham Domínguez-Avila Shela Gorinstein Gustavo A. González-Aguilar Rosario Maribel Robles-Sánchez Preventive Gastroprotective Effect of a Functional Food Based on Quinoa (<i>Chenopodium quinoa</i> Willd.) and Quercetin in a Murine Model of Ibuprofen-Induced Gastric Damage Antioxidants oxidative stress gastric lesion pseudocereals gastroprotection flavonoids |
| title | Preventive Gastroprotective Effect of a Functional Food Based on Quinoa (<i>Chenopodium quinoa</i> Willd.) and Quercetin in a Murine Model of Ibuprofen-Induced Gastric Damage |
| title_full | Preventive Gastroprotective Effect of a Functional Food Based on Quinoa (<i>Chenopodium quinoa</i> Willd.) and Quercetin in a Murine Model of Ibuprofen-Induced Gastric Damage |
| title_fullStr | Preventive Gastroprotective Effect of a Functional Food Based on Quinoa (<i>Chenopodium quinoa</i> Willd.) and Quercetin in a Murine Model of Ibuprofen-Induced Gastric Damage |
| title_full_unstemmed | Preventive Gastroprotective Effect of a Functional Food Based on Quinoa (<i>Chenopodium quinoa</i> Willd.) and Quercetin in a Murine Model of Ibuprofen-Induced Gastric Damage |
| title_short | Preventive Gastroprotective Effect of a Functional Food Based on Quinoa (<i>Chenopodium quinoa</i> Willd.) and Quercetin in a Murine Model of Ibuprofen-Induced Gastric Damage |
| title_sort | preventive gastroprotective effect of a functional food based on quinoa i chenopodium quinoa i willd and quercetin in a murine model of ibuprofen induced gastric damage |
| topic | oxidative stress gastric lesion pseudocereals gastroprotection flavonoids |
| url | https://www.mdpi.com/2076-3921/14/7/893 |
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