DETECCIÓN DE ROTAVIRUS BOVINO DEL GRUPO A. ANÁLISIS FILOGENÉTICO DEL GEN QUE CODIFICA PARA LA PROTEÍNA VP7 DE SU CÁPSIDE EXTERNA
Rotaviruses of group A are the major cause of diarrhea in young calves. To investigate the percentage ofgroup A rotaviruses in newborn calves and examine their evolutionary relationship with those from otheranimals and humans, we collected 74 fecal specimens from calves of 0-1 month of age with acut...
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Pontificia Universidad Javeriana
2007-12-01
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| Series: | Universitas Scientiarum |
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| Online Access: | https://docs.google.com/folder/d/0B_IdlsctkRrxVV8yVEVfRG1BQmM/edit?usp=sharing&pli=1&docId=0B_IdlsctkRrxZ3VBNkI0ZzZhdDg |
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| author | M.E. Guriérrez J. Baoming |
| author_facet | M.E. Guriérrez J. Baoming |
| author_sort | M.E. Guriérrez |
| collection | DOAJ |
| description | Rotaviruses of group A are the major cause of diarrhea in young calves. To investigate the percentage ofgroup A rotaviruses in newborn calves and examine their evolutionary relationship with those from otheranimals and humans, we collected 74 fecal specimens from calves of 0-1 month of age with acute diarrheain towns of Colombia. Using an enzyme-linked immunosorbent assay was found that 7% of specimenswere positive for rotaviruses of group A. A representative strain (UJ) was chosen to determine its nucleotide and to deduce amino acid sequences of VP7 gene. Nucleotide sequence of UJ VP7 gene was aligned withcorresponding sequences from 15 other strains, including 2 from cattle, 4 from swine, 6 from humans,and 1 each from chickens, dogs, and rabbits, by using Clustal W. Phylogenetic analysis was performedwith the MEGA analytical package. UJ strain clusters with a group A rotavirus strain (SI-B8) recentlyisolated from a calf in Slovenia in the neighbor-joining tree. UJ VP7 gene shares 84% and 70% amino acidsequence identity with the corresponding gene of the recently isolated bovine strains SI-B8 in Sloveniaand PP-1 in the United Kingdom. Identities in amino acid sequence between 48-73% were found withrotavirus of group A strains from humans and other animals, including swine, dogs, and rabbits. To ourknowledge, this is the first study to detect and characterize a bovine group A rotavirus in Colombia.Findings may increase our understanding of burden of viral gastroenteritis in calves and allow us todevelop strategies to prevent and control this important livestock disease. |
| format | Article |
| id | doaj-art-6d647b5f15fc4eb89ed1079a2394a3f7 |
| institution | Kabale University |
| issn | 0122-7483 2027-1352 |
| language | English |
| publishDate | 2007-12-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj-art-6d647b5f15fc4eb89ed1079a2394a3f72025-08-20T03:52:02ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522007-12-0112Edición Especial III3746DETECCIÓN DE ROTAVIRUS BOVINO DEL GRUPO A. ANÁLISIS FILOGENÉTICO DEL GEN QUE CODIFICA PARA LA PROTEÍNA VP7 DE SU CÁPSIDE EXTERNAM.E. GuriérrezJ. BaomingRotaviruses of group A are the major cause of diarrhea in young calves. To investigate the percentage ofgroup A rotaviruses in newborn calves and examine their evolutionary relationship with those from otheranimals and humans, we collected 74 fecal specimens from calves of 0-1 month of age with acute diarrheain towns of Colombia. Using an enzyme-linked immunosorbent assay was found that 7% of specimenswere positive for rotaviruses of group A. A representative strain (UJ) was chosen to determine its nucleotide and to deduce amino acid sequences of VP7 gene. Nucleotide sequence of UJ VP7 gene was aligned withcorresponding sequences from 15 other strains, including 2 from cattle, 4 from swine, 6 from humans,and 1 each from chickens, dogs, and rabbits, by using Clustal W. Phylogenetic analysis was performedwith the MEGA analytical package. UJ strain clusters with a group A rotavirus strain (SI-B8) recentlyisolated from a calf in Slovenia in the neighbor-joining tree. UJ VP7 gene shares 84% and 70% amino acidsequence identity with the corresponding gene of the recently isolated bovine strains SI-B8 in Sloveniaand PP-1 in the United Kingdom. Identities in amino acid sequence between 48-73% were found withrotavirus of group A strains from humans and other animals, including swine, dogs, and rabbits. To ourknowledge, this is the first study to detect and characterize a bovine group A rotavirus in Colombia.Findings may increase our understanding of burden of viral gastroenteritis in calves and allow us todevelop strategies to prevent and control this important livestock disease.https://docs.google.com/folder/d/0B_IdlsctkRrxVV8yVEVfRG1BQmM/edit?usp=sharing&pli=1&docId=0B_IdlsctkRrxZ3VBNkI0ZzZhdDgbovine rotavirusesVP7 gene |
| spellingShingle | M.E. Guriérrez J. Baoming DETECCIÓN DE ROTAVIRUS BOVINO DEL GRUPO A. ANÁLISIS FILOGENÉTICO DEL GEN QUE CODIFICA PARA LA PROTEÍNA VP7 DE SU CÁPSIDE EXTERNA Universitas Scientiarum bovine rotaviruses VP7 gene |
| title | DETECCIÓN DE ROTAVIRUS BOVINO DEL GRUPO A. ANÁLISIS FILOGENÉTICO DEL GEN QUE CODIFICA PARA LA PROTEÍNA VP7 DE SU CÁPSIDE EXTERNA |
| title_full | DETECCIÓN DE ROTAVIRUS BOVINO DEL GRUPO A. ANÁLISIS FILOGENÉTICO DEL GEN QUE CODIFICA PARA LA PROTEÍNA VP7 DE SU CÁPSIDE EXTERNA |
| title_fullStr | DETECCIÓN DE ROTAVIRUS BOVINO DEL GRUPO A. ANÁLISIS FILOGENÉTICO DEL GEN QUE CODIFICA PARA LA PROTEÍNA VP7 DE SU CÁPSIDE EXTERNA |
| title_full_unstemmed | DETECCIÓN DE ROTAVIRUS BOVINO DEL GRUPO A. ANÁLISIS FILOGENÉTICO DEL GEN QUE CODIFICA PARA LA PROTEÍNA VP7 DE SU CÁPSIDE EXTERNA |
| title_short | DETECCIÓN DE ROTAVIRUS BOVINO DEL GRUPO A. ANÁLISIS FILOGENÉTICO DEL GEN QUE CODIFICA PARA LA PROTEÍNA VP7 DE SU CÁPSIDE EXTERNA |
| title_sort | deteccion de rotavirus bovino del grupo a analisis filogenetico del gen que codifica para la proteina vp7 de su capside externa |
| topic | bovine rotaviruses VP7 gene |
| url | https://docs.google.com/folder/d/0B_IdlsctkRrxVV8yVEVfRG1BQmM/edit?usp=sharing&pli=1&docId=0B_IdlsctkRrxZ3VBNkI0ZzZhdDg |
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