Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics
Background. Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. Method...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/1429074 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832561771902140416 |
|---|---|
| author | Lili Xu Yang Jiao Weiliang Cui Bing Wang Dongxiao Guo Fei Xue Xiangrong Mu Huifen Li Yongqiang Lin Huibin Lin |
| author_facet | Lili Xu Yang Jiao Weiliang Cui Bing Wang Dongxiao Guo Fei Xue Xiangrong Mu Huifen Li Yongqiang Lin Huibin Lin |
| author_sort | Lili Xu |
| collection | DOAJ |
| description | Background. Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. Methods. We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. Results. The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. Conclusion. The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation. |
| format | Article |
| id | doaj-art-6d5f615211b04f24897f7efbde271afd |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-6d5f615211b04f24897f7efbde271afd2025-02-03T01:24:10ZengWileyJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/1429074Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and ChemometricsLili Xu0Yang Jiao1Weiliang Cui2Bing Wang3Dongxiao Guo4Fei Xue5Xiangrong Mu6Huifen Li7Yongqiang Lin8Huibin Lin9Shandong University of Traditional Chinese MedicineShandong Institute of Food and Drug ControlShandong Institute of Food and Drug ControlShandong Institute of Food and Drug ControlShandong Institute of Food and Drug ControlShandong Institute of Food and Drug ControlShandong Institute of Food and Drug ControlShandong University of Traditional Chinese MedicineShandong Institute of Food and Drug ControlShandong Academy of Chinese MedicineBackground. Naolingsu capsule (NLSC) is a well-known traditional Chinese medicine (TCM) prescription in China. It is widely used to treat neurasthenia, insomnia, cardiovascular and cerebrovascular disease, and other diseases. However, its inalienable chemical groups have not been carried out. Methods. We first established the nontargeted investigation based on fingerprinting coupled with UHPLC-Q/TOF-MS/MS. Second, the quantitative methods based on HPLC-DAD and LC-MS/MS were connected to the synchronous quantitative assurance of eleven and fourteen marker compounds. Finally, the quantitative information was processed with SIMCA-P for differentiating the distinctive bunches of samples to screen the foremost appropriate chemical markers. Results. The similarity of HPLC fingerprints of 24 batches of NLSC samples was 0.645–0.992. In total, 37 flavonoids, 21 organic acids, 22 lignans, 13 saponins, and 20 other compounds were recognized in NLSC by the UHPLC-Q/TOF-MS/MS method. The quantitative determination was approved for linearity, discovery limits, accuracy, repeatability, soundness, and precision. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models accomplished the great classification of the samples from the five enterprises, respectively. Rehmannioside D (RD), methylophiopogonanone A (MPA), 3,6′-disinapoyl sucrose (DS), schisandrin B (SSB), epimedin C (EC), icariin (ICA), and jujuboside B (JB) were considered as the potential chemical markers for NLSC quality control. Conclusion. The experimental results illustrated that the combinative strategy was valuable for quick pharmaceutical quality assessment, which can potentially differentiate the origin, decide the realness, and assess the overall quality of the formulation.http://dx.doi.org/10.1155/2022/1429074 |
| spellingShingle | Lili Xu Yang Jiao Weiliang Cui Bing Wang Dongxiao Guo Fei Xue Xiangrong Mu Huifen Li Yongqiang Lin Huibin Lin Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics Journal of Analytical Methods in Chemistry |
| title | Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics |
| title_full | Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics |
| title_fullStr | Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics |
| title_full_unstemmed | Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics |
| title_short | Quality Evaluation of Traditional Chinese Medicine Prescription in Naolingsu Capsule Based on Combinative Method of Fingerprint, Quantitative Determination, and Chemometrics |
| title_sort | quality evaluation of traditional chinese medicine prescription in naolingsu capsule based on combinative method of fingerprint quantitative determination and chemometrics |
| url | http://dx.doi.org/10.1155/2022/1429074 |
| work_keys_str_mv | AT lilixu qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT yangjiao qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT weiliangcui qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT bingwang qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT dongxiaoguo qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT feixue qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT xiangrongmu qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT huifenli qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT yongqianglin qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics AT huibinlin qualityevaluationoftraditionalchinesemedicineprescriptioninnaolingsucapsulebasedoncombinativemethodoffingerprintquantitativedeterminationandchemometrics |