Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell Carcinomas
We analysed the expression of cyclins A2, B1, D1, and E1 by immunohistochemistry and numerical aberrations in CCND1 gene by fluorescence in situ hybridization technique in 67 primary oral squamous cell carcinomas (OSCC). Cyclin A2 expression was observed in 54 (83.1%) tumours, cyclin D1 in 58 (89.2%...
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Wiley
2018-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/2018/7253510 |
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| author | Luís Silva Monteiro Márcio Diniz-Freitas Saman Warnakulasuriya Tomás Garcia-Caballero Jerónimo Forteza-Vila Máximo Fraga |
| author_facet | Luís Silva Monteiro Márcio Diniz-Freitas Saman Warnakulasuriya Tomás Garcia-Caballero Jerónimo Forteza-Vila Máximo Fraga |
| author_sort | Luís Silva Monteiro |
| collection | DOAJ |
| description | We analysed the expression of cyclins A2, B1, D1, and E1 by immunohistochemistry and numerical aberrations in CCND1 gene by fluorescence in situ hybridization technique in 67 primary oral squamous cell carcinomas (OSCC). Cyclin A2 expression was observed in 54 (83.1%) tumours, cyclin D1 in 58 (89.2%), cyclin B1 in 39 (60%), and cyclin E in 21 (32.8%). CCND1 region analysis revealed 26 (43.3%) tumours with the presence of numerical aberrations which were correlated with cyclin D1 high expression (Rho = 0.48; p<0.001). Twenty-nine (45.3%) tumours were classified as high proliferative tumours assessed by Ki-67 protein expression and correlated with tumours with high expression of cyclin A2 (Rho = 0.30; p=0.016) and cyclin B1 (Rho = 0.37; p=0.003). In multivariate analysis for an overall five-year survival (OS), we found an adverse independent prognostic value for cyclin A2 high expression (p=0.031) and for advanced tumour stage (p<0.001). Our results confirm that several cyclins are commonly expressed in OSCC. CCND1 gene is abnormal in more than one-third of the cases and is frequently associated with cyclin D1 high expression. Moreover, cyclin A2 high expression is an independent indicator of worse OS suggesting that this protein may serve as a reliable biological marker to identify high-risk subgroups with poor prognosis. |
| format | Article |
| id | doaj-art-6d4dbbf2c96e43718a8f10bcb9950bd2 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Analytical Cellular Pathology |
| spelling | doaj-art-6d4dbbf2c96e43718a8f10bcb9950bd22025-08-20T03:33:51ZengWileyAnalytical Cellular Pathology2210-71772210-71852018-01-01201810.1155/2018/72535107253510Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell CarcinomasLuís Silva Monteiro0Márcio Diniz-Freitas1Saman Warnakulasuriya2Tomás Garcia-Caballero3Jerónimo Forteza-Vila4Máximo Fraga5Institute of Research and Advanced Training in Health Sciences and Technologies (IINFACTS), University Institute of Health Sciences (IUCS), CESPU, CP 4585-116 Paredes, PortugalMedical-Surgical Dentistry Research Group (OMEQUI), Health Research Institute of Santiago de Compostela (IDIS), University of Santiago de Compostela, CP 15782 Santiago, SpainOral Medicine Department, The Dental Institute, King’s College and the WHO Collaborating Centre for Oral Cancer, London SE5 9RW, UKMorphological Sciences Department, School of Medicine, University Clinical Hospital, University of Santiago de Compostela, CP 15782 Santiago, SpainInstituto Valenciano de Patología, Universidad Católica de Valencia y Área Mixta de Investigación Oncológica (Centro de Investigación Príncipe de Valencia-UCV), CP 46012 Valencia, SpainPathology Department, School of Medicine-University Clinical Hospital, University of Santiago de Compostela, CP 15706 Santiago, SpainWe analysed the expression of cyclins A2, B1, D1, and E1 by immunohistochemistry and numerical aberrations in CCND1 gene by fluorescence in situ hybridization technique in 67 primary oral squamous cell carcinomas (OSCC). Cyclin A2 expression was observed in 54 (83.1%) tumours, cyclin D1 in 58 (89.2%), cyclin B1 in 39 (60%), and cyclin E in 21 (32.8%). CCND1 region analysis revealed 26 (43.3%) tumours with the presence of numerical aberrations which were correlated with cyclin D1 high expression (Rho = 0.48; p<0.001). Twenty-nine (45.3%) tumours were classified as high proliferative tumours assessed by Ki-67 protein expression and correlated with tumours with high expression of cyclin A2 (Rho = 0.30; p=0.016) and cyclin B1 (Rho = 0.37; p=0.003). In multivariate analysis for an overall five-year survival (OS), we found an adverse independent prognostic value for cyclin A2 high expression (p=0.031) and for advanced tumour stage (p<0.001). Our results confirm that several cyclins are commonly expressed in OSCC. CCND1 gene is abnormal in more than one-third of the cases and is frequently associated with cyclin D1 high expression. Moreover, cyclin A2 high expression is an independent indicator of worse OS suggesting that this protein may serve as a reliable biological marker to identify high-risk subgroups with poor prognosis.http://dx.doi.org/10.1155/2018/7253510 |
| spellingShingle | Luís Silva Monteiro Márcio Diniz-Freitas Saman Warnakulasuriya Tomás Garcia-Caballero Jerónimo Forteza-Vila Máximo Fraga Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell Carcinomas Analytical Cellular Pathology |
| title | Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell Carcinomas |
| title_full | Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell Carcinomas |
| title_fullStr | Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell Carcinomas |
| title_full_unstemmed | Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell Carcinomas |
| title_short | Prognostic Significance of Cyclins A2, B1, D1, and E1 and CCND1 Numerical Aberrations in Oral Squamous Cell Carcinomas |
| title_sort | prognostic significance of cyclins a2 b1 d1 and e1 and ccnd1 numerical aberrations in oral squamous cell carcinomas |
| url | http://dx.doi.org/10.1155/2018/7253510 |
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