Exploring the predictive values of CRP and lymphocytes in coronary artery disease based on a machine learning and Mendelian randomization

PurposeTo investigate the predictive value of leukocyte subsets and C-reactive protein (CRP) in coronary artery disease (CAD).MethodsWe conducted a Mendelian randomization analysis (MR) on leukocyte subsets, C-reactive protein (CRP) and CAD, incorporating data from 68,624 patients who underwent coro...

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Main Authors: Yuan Liu, Xin Yuan, Yu-Chan He, Zhong-Hai Bi, Si-Yao Li, Ye Li, Yan-Li Liu, Liu Miao
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-09-01
Series:Frontiers in Cardiovascular Medicine
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Online Access:https://www.frontiersin.org/articles/10.3389/fcvm.2024.1442275/full
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author Yuan Liu
Yuan Liu
Xin Yuan
Xin Yuan
Yu-Chan He
Yu-Chan He
Zhong-Hai Bi
Zhong-Hai Bi
Si-Yao Li
Si-Yao Li
Ye Li
Ye Li
Yan-Li Liu
Yan-Li Liu
Liu Miao
Liu Miao
author_facet Yuan Liu
Yuan Liu
Xin Yuan
Xin Yuan
Yu-Chan He
Yu-Chan He
Zhong-Hai Bi
Zhong-Hai Bi
Si-Yao Li
Si-Yao Li
Ye Li
Ye Li
Yan-Li Liu
Yan-Li Liu
Liu Miao
Liu Miao
author_sort Yuan Liu
collection DOAJ
description PurposeTo investigate the predictive value of leukocyte subsets and C-reactive protein (CRP) in coronary artery disease (CAD).MethodsWe conducted a Mendelian randomization analysis (MR) on leukocyte subsets, C-reactive protein (CRP) and CAD, incorporating data from 68,624 patients who underwent coronary angiography from 2010 to 2022. After initial screening, clinical data from 46,664 patients were analyzed. Techniques employed included propensity score matching (PSM), logistic regression, lasso regression, and random forest algorithms (RF). Risk factors were assessed, and the sensitivity and specificity of the models were evaluated using receiver operating characteristic (ROC) curves. Additionally, survival analysis was conducted based on a 36-month follow-up period.ResultsThe inverse variance weight (IVW) analysis showed that basophil count (OR 0.92, 95% CI: 0.84–1.00, P = 0.048), CRP levels (OR 0.87, 95% CI: 0.73–1.00, P = 0.040), and lymphocyte count (OR 1.10, 95% CI: 1.04–1.16, P = 0.001) are significant risk factors for CAD. Using LASSO regression, logistic regression, and RF analysis, both CRP and lymphocyte counts were consistently identified as risk factors for CAD, prior to and following PSM. The ROC curve analysis indicated that the combination of lymphocyte and CRP levels after PSM achieves a higher diagnostic value (0.85). Survival analysis revealed that high lymphocyte counts and low CRP levels are associated with a decreased risk of Major Adverse Cardiovascular Events (MACE) (P < 0.001). Conversely, a higher CRP level combined with lymphocyte counts correlates with a poorer prognosis.ConclusionThere is a causal relationship between lymphocytes, CRP and CAD. The combined assessment of CRP and lymphocytes offers diagnostic value for CAD. Furthermore, high CRP levels coupled with low lymphocyte counts are associated with a poor prognosis.
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spelling doaj-art-6d3a3b21c0cb4fc2a1215e0fa28033942025-08-20T01:55:14ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2024-09-011110.3389/fcvm.2024.14422751442275Exploring the predictive values of CRP and lymphocytes in coronary artery disease based on a machine learning and Mendelian randomizationYuan Liu0Yuan Liu1Xin Yuan2Xin Yuan3Yu-Chan He4Yu-Chan He5Zhong-Hai Bi6Zhong-Hai Bi7Si-Yao Li8Si-Yao Li9Ye Li10Ye Li11Yan-Li Liu12Yan-Li Liu13Liu Miao14Liu Miao15Department of Cardiology, Liuzhou People’s Hospital, Affiliated of Guangxi Medical University, Liuzhou, Guangxi, ChinaThe Key Laboratory of Coronary Atherosclerotic Disease Prevention and Treatment of Liuzhou, Liuzhou, Guangxi, ChinaDepartment of Cardiology, Liuzhou People’s Hospital, Affiliated of Guangxi Medical University, Liuzhou, Guangxi, ChinaThe Key Laboratory of Coronary Atherosclerotic Disease Prevention and Treatment of Liuzhou, Liuzhou, Guangxi, ChinaDepartment of Cardiology, Liuzhou People’s Hospital, Affiliated of Guangxi Medical University, Liuzhou, Guangxi, ChinaThe Key Laboratory of Coronary Atherosclerotic Disease Prevention and Treatment of Liuzhou, Liuzhou, Guangxi, ChinaDepartment of Cardiology, Liuzhou People’s Hospital, Affiliated of Guangxi Medical University, Liuzhou, Guangxi, ChinaThe Key Laboratory of Coronary Atherosclerotic Disease Prevention and Treatment of Liuzhou, Liuzhou, Guangxi, ChinaDepartment of Cardiology, Liuzhou People’s Hospital, Affiliated of Guangxi Medical University, Liuzhou, Guangxi, ChinaThe Key Laboratory of Coronary Atherosclerotic Disease Prevention and Treatment of Liuzhou, Liuzhou, Guangxi, ChinaDepartment of Cardiology, Liuzhou People’s Hospital, Affiliated of Guangxi Medical University, Liuzhou, Guangxi, ChinaThe Key Laboratory of Coronary Atherosclerotic Disease Prevention and Treatment of Liuzhou, Liuzhou, Guangxi, ChinaDepartment of Cardiology, Liuzhou People’s Hospital, Affiliated of Guangxi Medical University, Liuzhou, Guangxi, ChinaThe Key Laboratory of Coronary Atherosclerotic Disease Prevention and Treatment of Liuzhou, Liuzhou, Guangxi, ChinaDepartment of Cardiology, Liuzhou People’s Hospital, Affiliated of Guangxi Medical University, Liuzhou, Guangxi, ChinaThe Key Laboratory of Coronary Atherosclerotic Disease Prevention and Treatment of Liuzhou, Liuzhou, Guangxi, ChinaPurposeTo investigate the predictive value of leukocyte subsets and C-reactive protein (CRP) in coronary artery disease (CAD).MethodsWe conducted a Mendelian randomization analysis (MR) on leukocyte subsets, C-reactive protein (CRP) and CAD, incorporating data from 68,624 patients who underwent coronary angiography from 2010 to 2022. After initial screening, clinical data from 46,664 patients were analyzed. Techniques employed included propensity score matching (PSM), logistic regression, lasso regression, and random forest algorithms (RF). Risk factors were assessed, and the sensitivity and specificity of the models were evaluated using receiver operating characteristic (ROC) curves. Additionally, survival analysis was conducted based on a 36-month follow-up period.ResultsThe inverse variance weight (IVW) analysis showed that basophil count (OR 0.92, 95% CI: 0.84–1.00, P = 0.048), CRP levels (OR 0.87, 95% CI: 0.73–1.00, P = 0.040), and lymphocyte count (OR 1.10, 95% CI: 1.04–1.16, P = 0.001) are significant risk factors for CAD. Using LASSO regression, logistic regression, and RF analysis, both CRP and lymphocyte counts were consistently identified as risk factors for CAD, prior to and following PSM. The ROC curve analysis indicated that the combination of lymphocyte and CRP levels after PSM achieves a higher diagnostic value (0.85). Survival analysis revealed that high lymphocyte counts and low CRP levels are associated with a decreased risk of Major Adverse Cardiovascular Events (MACE) (P < 0.001). Conversely, a higher CRP level combined with lymphocyte counts correlates with a poorer prognosis.ConclusionThere is a causal relationship between lymphocytes, CRP and CAD. The combined assessment of CRP and lymphocytes offers diagnostic value for CAD. Furthermore, high CRP levels coupled with low lymphocyte counts are associated with a poor prognosis.https://www.frontiersin.org/articles/10.3389/fcvm.2024.1442275/fullcoronary artery diseaselymphocytesCRPMendelian randomizationretrospective analysis
spellingShingle Yuan Liu
Yuan Liu
Xin Yuan
Xin Yuan
Yu-Chan He
Yu-Chan He
Zhong-Hai Bi
Zhong-Hai Bi
Si-Yao Li
Si-Yao Li
Ye Li
Ye Li
Yan-Li Liu
Yan-Li Liu
Liu Miao
Liu Miao
Exploring the predictive values of CRP and lymphocytes in coronary artery disease based on a machine learning and Mendelian randomization
Frontiers in Cardiovascular Medicine
coronary artery disease
lymphocytes
CRP
Mendelian randomization
retrospective analysis
title Exploring the predictive values of CRP and lymphocytes in coronary artery disease based on a machine learning and Mendelian randomization
title_full Exploring the predictive values of CRP and lymphocytes in coronary artery disease based on a machine learning and Mendelian randomization
title_fullStr Exploring the predictive values of CRP and lymphocytes in coronary artery disease based on a machine learning and Mendelian randomization
title_full_unstemmed Exploring the predictive values of CRP and lymphocytes in coronary artery disease based on a machine learning and Mendelian randomization
title_short Exploring the predictive values of CRP and lymphocytes in coronary artery disease based on a machine learning and Mendelian randomization
title_sort exploring the predictive values of crp and lymphocytes in coronary artery disease based on a machine learning and mendelian randomization
topic coronary artery disease
lymphocytes
CRP
Mendelian randomization
retrospective analysis
url https://www.frontiersin.org/articles/10.3389/fcvm.2024.1442275/full
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