Study Protocol: Evaluation of AI-Driven Grading Compared to Manual Grading in Predicting Embryo Viability and Successful Implantation and Clinical Pregnancy Outcomes in IVF Using Static Microscopic Images

Background: Infertility affects 8–12% of couples globally, with 5–10% seeking Assisted Reproductive Technology (ART) annually. In-vitro fertilization (IVF) is the most common infertility treatment, involving the retrieval of oocytes and their fertilization in a laboratory setting. Embryo selection,...

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Main Authors: Puja Dhamija, Akash More, Namrata Choudhary, Tejaswini Wadhe, Devanshi R. Shah
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-05-01
Series:Journal of Pharmacy and Bioallied Sciences
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Online Access:https://journals.lww.com/10.4103/jpbs.jpbs_386_25
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author Puja Dhamija
Akash More
Namrata Choudhary
Tejaswini Wadhe
Devanshi R. Shah
author_facet Puja Dhamija
Akash More
Namrata Choudhary
Tejaswini Wadhe
Devanshi R. Shah
author_sort Puja Dhamija
collection DOAJ
description Background: Infertility affects 8–12% of couples globally, with 5–10% seeking Assisted Reproductive Technology (ART) annually. In-vitro fertilization (IVF) is the most common infertility treatment, involving the retrieval of oocytes and their fertilization in a laboratory setting. Embryo selection, crucial for IVF success, is traditionally performed manually by embryologists using the Gardner Scale. However, this process is subject to variability. Time-lapse microscopy and artificial intelligence (AI)-based methods are being explored for improved embryo selection, though AI’s full potential has not been realized across diverse clinical settings. Objectives: The primary objective of this study is to compare AI-based embryo grading with conventional manual grading by embryologists in predicting clinical pregnancy outcomes. Methodology: This prospective study will be conducted at an IVF clinic in Sawangi, Wardha, Maharashtra, involving 222 participants aged 23–40 years undergoing Intra-Cytoplasmic Sperm Injection (ICSI). Embryos on Day 5 (blastocyst stage) will be imaged and graded using Life Whisperer Genetics (LWG), an AI-based tool, and by skilled embryologists using the ASEBIR criteria. The success rate of clinical pregnancy, confirmed by the presence of a gestational sac, will be the primary outcome. Expected Results: The study is expected to show increased predictive efficiency, rigor, and consistency with AI-driven grading of Day 5 embryos, providing a more economical solution for IVF. Study Implications: This study focuses on enhancing embryo selection using Life Whisperer Genetics (LWG), which could potentially improve embryo assessment in IVF. Further research is needed to incorporate other embryonic developmental stages for a more comprehensive evaluation process.
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spelling doaj-art-6d397690aeb0476d837f6aba90f4310f2025-08-20T03:07:49ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062025-05-0117Suppl 1S956S95910.4103/jpbs.jpbs_386_25Study Protocol: Evaluation of AI-Driven Grading Compared to Manual Grading in Predicting Embryo Viability and Successful Implantation and Clinical Pregnancy Outcomes in IVF Using Static Microscopic ImagesPuja DhamijaAkash MoreNamrata ChoudharyTejaswini WadheDevanshi R. ShahBackground: Infertility affects 8–12% of couples globally, with 5–10% seeking Assisted Reproductive Technology (ART) annually. In-vitro fertilization (IVF) is the most common infertility treatment, involving the retrieval of oocytes and their fertilization in a laboratory setting. Embryo selection, crucial for IVF success, is traditionally performed manually by embryologists using the Gardner Scale. However, this process is subject to variability. Time-lapse microscopy and artificial intelligence (AI)-based methods are being explored for improved embryo selection, though AI’s full potential has not been realized across diverse clinical settings. Objectives: The primary objective of this study is to compare AI-based embryo grading with conventional manual grading by embryologists in predicting clinical pregnancy outcomes. Methodology: This prospective study will be conducted at an IVF clinic in Sawangi, Wardha, Maharashtra, involving 222 participants aged 23–40 years undergoing Intra-Cytoplasmic Sperm Injection (ICSI). Embryos on Day 5 (blastocyst stage) will be imaged and graded using Life Whisperer Genetics (LWG), an AI-based tool, and by skilled embryologists using the ASEBIR criteria. The success rate of clinical pregnancy, confirmed by the presence of a gestational sac, will be the primary outcome. Expected Results: The study is expected to show increased predictive efficiency, rigor, and consistency with AI-driven grading of Day 5 embryos, providing a more economical solution for IVF. Study Implications: This study focuses on enhancing embryo selection using Life Whisperer Genetics (LWG), which could potentially improve embryo assessment in IVF. Further research is needed to incorporate other embryonic developmental stages for a more comprehensive evaluation process.https://journals.lww.com/10.4103/jpbs.jpbs_386_25artificial intelligenceassisted reproductive technologyembryo gradingembryo selectioninfertilityivflife whisperer geneticspregnancy outcomes
spellingShingle Puja Dhamija
Akash More
Namrata Choudhary
Tejaswini Wadhe
Devanshi R. Shah
Study Protocol: Evaluation of AI-Driven Grading Compared to Manual Grading in Predicting Embryo Viability and Successful Implantation and Clinical Pregnancy Outcomes in IVF Using Static Microscopic Images
Journal of Pharmacy and Bioallied Sciences
artificial intelligence
assisted reproductive technology
embryo grading
embryo selection
infertility
ivf
life whisperer genetics
pregnancy outcomes
title Study Protocol: Evaluation of AI-Driven Grading Compared to Manual Grading in Predicting Embryo Viability and Successful Implantation and Clinical Pregnancy Outcomes in IVF Using Static Microscopic Images
title_full Study Protocol: Evaluation of AI-Driven Grading Compared to Manual Grading in Predicting Embryo Viability and Successful Implantation and Clinical Pregnancy Outcomes in IVF Using Static Microscopic Images
title_fullStr Study Protocol: Evaluation of AI-Driven Grading Compared to Manual Grading in Predicting Embryo Viability and Successful Implantation and Clinical Pregnancy Outcomes in IVF Using Static Microscopic Images
title_full_unstemmed Study Protocol: Evaluation of AI-Driven Grading Compared to Manual Grading in Predicting Embryo Viability and Successful Implantation and Clinical Pregnancy Outcomes in IVF Using Static Microscopic Images
title_short Study Protocol: Evaluation of AI-Driven Grading Compared to Manual Grading in Predicting Embryo Viability and Successful Implantation and Clinical Pregnancy Outcomes in IVF Using Static Microscopic Images
title_sort study protocol evaluation of ai driven grading compared to manual grading in predicting embryo viability and successful implantation and clinical pregnancy outcomes in ivf using static microscopic images
topic artificial intelligence
assisted reproductive technology
embryo grading
embryo selection
infertility
ivf
life whisperer genetics
pregnancy outcomes
url https://journals.lww.com/10.4103/jpbs.jpbs_386_25
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