Input–output organization of the mouse BLA-projecting IL neurons
IntroductionThe infralimbic cortex (IL), a critical subregion within the ventromedial prefrontal cortex (vmPFC), modulates emotion, memory, and social functions via robust projections to the basolateral amygdala (BLA). However, the afferent brain regions that innervate BLA-projecting IL neurons (IL-...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2025.1532078/full |
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| author | Zhuo Li Zhuo Li Yu Qu Yu Qu Qi-Lin Wu Li Tang Yuan Dong Yuan Dong Xu-Feng Xu Xu-Feng Xu |
| author_facet | Zhuo Li Zhuo Li Yu Qu Yu Qu Qi-Lin Wu Li Tang Yuan Dong Yuan Dong Xu-Feng Xu Xu-Feng Xu |
| author_sort | Zhuo Li |
| collection | DOAJ |
| description | IntroductionThe infralimbic cortex (IL), a critical subregion within the ventromedial prefrontal cortex (vmPFC), modulates emotion, memory, and social functions via robust projections to the basolateral amygdala (BLA). However, the afferent brain regions that innervate BLA-projecting IL neurons (IL-BLA PNs) and their efferent targets have not been systematically characterized.MethodsWe employed tracing techniques integrating adeno-associated virus (AAV) and monosynaptic rabies to systematically investigate the presynaptic inputs and axonal outputs of IL-BLA PNs.ResultsOur findings revealed widespread synaptic inputs to IL-BLA PNs from subcortical areas, with the strongest inputs originating from the dorsomedial thalamus (MD) and anterior medial thalamus (AM) in the thalamic region, as well as from the ventral hippocampus (vHPC) and piriform cortex (Pir) in the limbic system. Sparse labeling of the virus demonstrated that IL-BLA PNs collateralize to innervate various subcortical regions, including the horizontal diagonal band (HDB), lateral preoptic area (LPO), ventral pallidum (VP), and lateral hypothalamus (LH).DiscussionThis work establishes essential theoretical frameworks for the functional investigation and targeted intervention in neurological disorders. |
| format | Article |
| id | doaj-art-6d36d219d9a34bb1bf4e0caf8c33c728 |
| institution | DOAJ |
| issn | 1662-453X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Neuroscience |
| spelling | doaj-art-6d36d219d9a34bb1bf4e0caf8c33c7282025-08-20T03:11:49ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2025-02-011910.3389/fnins.2025.15320781532078Input–output organization of the mouse BLA-projecting IL neuronsZhuo Li0Zhuo Li1Yu Qu2Yu Qu3Qi-Lin Wu4Li Tang5Yuan Dong6Yuan Dong7Xu-Feng Xu8Xu-Feng Xu9Basic School of Medicine, Qingdao Medical College, Qingdao University, Qingdao, ChinaInstitute of Neuropsychiatric Diseases, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaQilu Hospital of Shandong University, Jinan, ChinaSchool of Rehabilitation Science and Engineering Qingdao National High-tech Industrial Development Zone, University of Health and Rehabilitation Sciences, Qingdao, ChinaBasic School of Medicine, Qingdao Medical College, Qingdao University, Qingdao, ChinaQingdao Institute of Measurement Technology, Qingdao, ChinaBasic School of Medicine, Qingdao Medical College, Qingdao University, Qingdao, ChinaInstitute of Neuropsychiatric Diseases, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaBasic School of Medicine, Qingdao Medical College, Qingdao University, Qingdao, ChinaInstitute of Neuropsychiatric Diseases, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, ChinaIntroductionThe infralimbic cortex (IL), a critical subregion within the ventromedial prefrontal cortex (vmPFC), modulates emotion, memory, and social functions via robust projections to the basolateral amygdala (BLA). However, the afferent brain regions that innervate BLA-projecting IL neurons (IL-BLA PNs) and their efferent targets have not been systematically characterized.MethodsWe employed tracing techniques integrating adeno-associated virus (AAV) and monosynaptic rabies to systematically investigate the presynaptic inputs and axonal outputs of IL-BLA PNs.ResultsOur findings revealed widespread synaptic inputs to IL-BLA PNs from subcortical areas, with the strongest inputs originating from the dorsomedial thalamus (MD) and anterior medial thalamus (AM) in the thalamic region, as well as from the ventral hippocampus (vHPC) and piriform cortex (Pir) in the limbic system. Sparse labeling of the virus demonstrated that IL-BLA PNs collateralize to innervate various subcortical regions, including the horizontal diagonal band (HDB), lateral preoptic area (LPO), ventral pallidum (VP), and lateral hypothalamus (LH).DiscussionThis work establishes essential theoretical frameworks for the functional investigation and targeted intervention in neurological disorders.https://www.frontiersin.org/articles/10.3389/fnins.2025.1532078/fullthe ventromedial prefrontal cortexbasolateral amygdalaAAVrabiesneurons |
| spellingShingle | Zhuo Li Zhuo Li Yu Qu Yu Qu Qi-Lin Wu Li Tang Yuan Dong Yuan Dong Xu-Feng Xu Xu-Feng Xu Input–output organization of the mouse BLA-projecting IL neurons Frontiers in Neuroscience the ventromedial prefrontal cortex basolateral amygdala AAV rabies neurons |
| title | Input–output organization of the mouse BLA-projecting IL neurons |
| title_full | Input–output organization of the mouse BLA-projecting IL neurons |
| title_fullStr | Input–output organization of the mouse BLA-projecting IL neurons |
| title_full_unstemmed | Input–output organization of the mouse BLA-projecting IL neurons |
| title_short | Input–output organization of the mouse BLA-projecting IL neurons |
| title_sort | input output organization of the mouse bla projecting il neurons |
| topic | the ventromedial prefrontal cortex basolateral amygdala AAV rabies neurons |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2025.1532078/full |
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