Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease
The etiology and pathogenesis of rheumatoid arthritis (RA) are marked by a complex interplay of various cell populations and is mediated by different signaling pathways. Traditionally, therapies have primarily focused on pain relief, reducing inflammation and the recovery of joint function. More rec...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2017/1046438 |
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| author | Mersedeh Tohidnezhad Andreas Bayer Biljana Rasuo Jennifer Vanessa Phi Hock Nisreen Kweider Athanassios Fragoulis Tolga Taha Sönmez Holger Jahr Thomas Pufe Sebastian Lippross |
| author_facet | Mersedeh Tohidnezhad Andreas Bayer Biljana Rasuo Jennifer Vanessa Phi Hock Nisreen Kweider Athanassios Fragoulis Tolga Taha Sönmez Holger Jahr Thomas Pufe Sebastian Lippross |
| author_sort | Mersedeh Tohidnezhad |
| collection | DOAJ |
| description | The etiology and pathogenesis of rheumatoid arthritis (RA) are marked by a complex interplay of various cell populations and is mediated by different signaling pathways. Traditionally, therapies have primarily focused on pain relief, reducing inflammation and the recovery of joint function. More recently, however, researchers have discussed the therapeutic efficacy of autologous platelet-rich plasma (PRP). The main objective of this work is to examine the influences of platelet-released growth factor (PRGF) on human synoviocytes under inflammatory conditions. Additionally, it is checked to which extend treatment with platelet concentrate influences the release of cytokines form synoviocytes. For this purpose, an in vitro RA model was created by stimulating the cells with the TNF-α. The release of cytokines was measured by ELISA. The cytokine gene expression was analyzed by real-time PCR. It has been observed that the stimulation concentration of 10 ng/ml TNF-α resulted in a significantly increased endogenous secretion and gene expression of IL-6 and TNF-α. The anti-inflammatory effect of PRGF could be confirmed through significant reduction of TNF-α and IL-1β. An induced inflammatory condition seems to cause PRGF to inhibit the release of proinflammatory cytokines. Further study is required to understand the exact effect mechanism of PRGF on synoviocytes. |
| format | Article |
| id | doaj-art-6d33c71397ea433e91905385cc7fe0bf |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6d33c71397ea433e91905385cc7fe0bf2025-08-20T02:06:44ZengWileyMediators of Inflammation0962-93511466-18612017-01-01201710.1155/2017/10464381046438Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint DiseaseMersedeh Tohidnezhad0Andreas Bayer1Biljana Rasuo2Jennifer Vanessa Phi Hock3Nisreen Kweider4Athanassios Fragoulis5Tolga Taha Sönmez6Holger Jahr7Thomas Pufe8Sebastian Lippross9Institute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, GermanyDepartment of Heart and Vascular Surgery, University Hospital of Schleswig-Holstein, Kiel, GermanyInstitute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, GermanyInstitute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, GermanyInstitute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, GermanyInstitute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, GermanyInstitute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, GermanyDepartment of Orthopaedic Surgery, RWTH Aachen University, Pauwelsstr. 30, 52074 Aachen, GermanyInstitute of Anatomy and Cell Biology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, GermanyDepartment of Traumatology, University Hospital of Schleswig-Holstein, Kiel, GermanyThe etiology and pathogenesis of rheumatoid arthritis (RA) are marked by a complex interplay of various cell populations and is mediated by different signaling pathways. Traditionally, therapies have primarily focused on pain relief, reducing inflammation and the recovery of joint function. More recently, however, researchers have discussed the therapeutic efficacy of autologous platelet-rich plasma (PRP). The main objective of this work is to examine the influences of platelet-released growth factor (PRGF) on human synoviocytes under inflammatory conditions. Additionally, it is checked to which extend treatment with platelet concentrate influences the release of cytokines form synoviocytes. For this purpose, an in vitro RA model was created by stimulating the cells with the TNF-α. The release of cytokines was measured by ELISA. The cytokine gene expression was analyzed by real-time PCR. It has been observed that the stimulation concentration of 10 ng/ml TNF-α resulted in a significantly increased endogenous secretion and gene expression of IL-6 and TNF-α. The anti-inflammatory effect of PRGF could be confirmed through significant reduction of TNF-α and IL-1β. An induced inflammatory condition seems to cause PRGF to inhibit the release of proinflammatory cytokines. Further study is required to understand the exact effect mechanism of PRGF on synoviocytes.http://dx.doi.org/10.1155/2017/1046438 |
| spellingShingle | Mersedeh Tohidnezhad Andreas Bayer Biljana Rasuo Jennifer Vanessa Phi Hock Nisreen Kweider Athanassios Fragoulis Tolga Taha Sönmez Holger Jahr Thomas Pufe Sebastian Lippross Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease Mediators of Inflammation |
| title | Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease |
| title_full | Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease |
| title_fullStr | Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease |
| title_full_unstemmed | Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease |
| title_short | Platelet-Released Growth Factors Modulate the Secretion of Cytokines in Synoviocytes under Inflammatory Joint Disease |
| title_sort | platelet released growth factors modulate the secretion of cytokines in synoviocytes under inflammatory joint disease |
| url | http://dx.doi.org/10.1155/2017/1046438 |
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