Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars
Genomic DNA was extracted from tissue specimens of wild boars (Sus scrofa) in 10 different locations Mongolia. D-loop part of genome was amplified by PCR and was subjected to DNA sequencing. Determined sequences from 18 specimens were registered with the GenBank and accession numbers were obtained....
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Mongolian Academy of Sciences
2022-12-01
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| Series: | Proceedings of the Mongolian Academy of Sciences |
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| Online Access: | https://www.mongoliajol.info/index.php/PMAS/article/view/2472 |
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| author | Ali Khamit Munkhjargal Bayarlkhagva Davaa Bazarsad Bolortuya Ulziibat Bayarlkhagva Damdin Bayarmaa Gun-Aajav |
| author_facet | Ali Khamit Munkhjargal Bayarlkhagva Davaa Bazarsad Bolortuya Ulziibat Bayarlkhagva Damdin Bayarmaa Gun-Aajav |
| author_sort | Ali Khamit |
| collection | DOAJ |
| description | Genomic DNA was extracted from tissue specimens of wild boars (Sus scrofa) in 10 different locations Mongolia. D-loop part of genome was amplified by PCR and was subjected to DNA sequencing. Determined sequences from 18 specimens were registered with the GenBank and accession numbers were obtained. A total of 54 complete mitochondrial D-loop sequences of wild boars available with NCBI GenBank were taken as a reference for comparison with that of Mongolian wild boars. Sequence alignment, detection of parsimonious informative sites, model selection, calculation of nucleotide distances, and Maximum Likelihood (ML) phylogenetic tree construction with 1000 bootstrapped replications were conducted using MEGA X software. Maximum Likelihood trees were constructed by the Hasegawa-Kishino-Yano (HKY) model. The results of the study showed that geographic location played an important role in sequence divergence between wild boars from various locations. Most of them were grouped together according to their respective geographic locations, except for several individuals. It is highly likely that the Mongolian subpopulation of wild boars, such as S. scrofa raddianus and S. scrofa nigripes, have had the same ancestor. In order to fully evaluate the distribution, ecology, and biology of Mongolian wild boars, it is essential to compare supplemental gene sequences that can reveal phylogenetic differences from the populations in the neighboring areas, such as Russia, northeast China, and Kazakhstan. The results of this study will be useful and informative for the protecting and conserving of wild boars in Mongolia. |
| format | Article |
| id | doaj-art-6d2dc04efec346498e30f06e5fc5e73d |
| institution | Kabale University |
| issn | 2310-4716 2312-2994 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Mongolian Academy of Sciences |
| record_format | Article |
| series | Proceedings of the Mongolian Academy of Sciences |
| spelling | doaj-art-6d2dc04efec346498e30f06e5fc5e73d2025-08-20T03:25:27ZengMongolian Academy of SciencesProceedings of the Mongolian Academy of Sciences2310-47162312-29942022-12-01223110.5564/pmas.v62i03.24722423Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boarsAli Khamit0https://orcid.org/0000-0002-5405-7702Munkhjargal Bayarlkhagva1https://orcid.org/0000-0002-6416-8916Davaa Bazarsad2https://orcid.org/0000-0003-3644-8651Bolortuya Ulziibat3https://orcid.org/0000-0002-7394-4833Bayarlkhagva Damdin4https://orcid.org/0000-0002-1896-5172Bayarmaa Gun-Aajav5https://orcid.org/0000-0001-6550-657XDepartment of Biology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar, MongoliaZoological Society of London’s Representative Office in Mongolia, Ulaanbaatar, MongoliaDepartment of Scientific Analysis, National Institute of Forensic Science, Ulaanbaatar, MongoliaPlant biotechnology laboratory, Institute of Biology, Mongolian Academy of Sciences, Ulaanbaatar, MongoliaDepartment of Biology, School of Arts and Sciences, National University of Mongolia, Ulaanbaatar, MongoliaDepartment of Biology, School of Arts and Sciences, National University of Mongolia, UlaanbaatarGenomic DNA was extracted from tissue specimens of wild boars (Sus scrofa) in 10 different locations Mongolia. D-loop part of genome was amplified by PCR and was subjected to DNA sequencing. Determined sequences from 18 specimens were registered with the GenBank and accession numbers were obtained. A total of 54 complete mitochondrial D-loop sequences of wild boars available with NCBI GenBank were taken as a reference for comparison with that of Mongolian wild boars. Sequence alignment, detection of parsimonious informative sites, model selection, calculation of nucleotide distances, and Maximum Likelihood (ML) phylogenetic tree construction with 1000 bootstrapped replications were conducted using MEGA X software. Maximum Likelihood trees were constructed by the Hasegawa-Kishino-Yano (HKY) model. The results of the study showed that geographic location played an important role in sequence divergence between wild boars from various locations. Most of them were grouped together according to their respective geographic locations, except for several individuals. It is highly likely that the Mongolian subpopulation of wild boars, such as S. scrofa raddianus and S. scrofa nigripes, have had the same ancestor. In order to fully evaluate the distribution, ecology, and biology of Mongolian wild boars, it is essential to compare supplemental gene sequences that can reveal phylogenetic differences from the populations in the neighboring areas, such as Russia, northeast China, and Kazakhstan. The results of this study will be useful and informative for the protecting and conserving of wild boars in Mongolia.https://www.mongoliajol.info/index.php/PMAS/article/view/2472sus scrofad-loopphylogenetic analysismitochondrial dna |
| spellingShingle | Ali Khamit Munkhjargal Bayarlkhagva Davaa Bazarsad Bolortuya Ulziibat Bayarlkhagva Damdin Bayarmaa Gun-Aajav Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars Proceedings of the Mongolian Academy of Sciences sus scrofa d-loop phylogenetic analysis mitochondrial dna |
| title | Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars |
| title_full | Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars |
| title_fullStr | Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars |
| title_full_unstemmed | Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars |
| title_short | Phylogenetic analysis of mitochondrial D-loop sequence of Mongolian wild boars |
| title_sort | phylogenetic analysis of mitochondrial d loop sequence of mongolian wild boars |
| topic | sus scrofa d-loop phylogenetic analysis mitochondrial dna |
| url | https://www.mongoliajol.info/index.php/PMAS/article/view/2472 |
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