Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cells
Recently, increasing interest has been in utilizing mesenchymal stem cell-derived extracellular vesicles (MSC-EVs), especially exosomes, as nanocarriers for miRNA delivery in cancer treatment. Due to such characteristics, nanocarriers are specific: biocompatible, low immunogenicity, and capable of s...
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Elsevier
2025-03-01
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| Series: | Regenerative Therapy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352320425000197 |
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| author | Ashok Kumar Balaraman M. Arockia Babu Muhammad Afzal Gaurav Sanghvi Rekha M M Sofia Gupta Mohit Rana Haider Ali Kavita Goyal Vetriselvan Subramaniyan Ling Shing Wong Vinoth Kumarasamy |
| author_facet | Ashok Kumar Balaraman M. Arockia Babu Muhammad Afzal Gaurav Sanghvi Rekha M M Sofia Gupta Mohit Rana Haider Ali Kavita Goyal Vetriselvan Subramaniyan Ling Shing Wong Vinoth Kumarasamy |
| author_sort | Ashok Kumar Balaraman |
| collection | DOAJ |
| description | Recently, increasing interest has been in utilizing mesenchymal stem cell-derived extracellular vesicles (MSC-EVs), especially exosomes, as nanocarriers for miRNA delivery in cancer treatment. Due to such characteristics, nanocarriers are specific: biocompatible, low immunogenicity, and capable of spontaneous tumor accumulation. MSC-EVs were loaded with therapeutic miRNAs and minimized their susceptibility to degradation by protecting the miRNA from accessibility to degrading enzymes and providing targeted delivery of the miRNAs to the tumor cells to modulate oncogenic pathways. In vitro and in vivo experiments suggest that MSC-EVs loaded with miRNAs may inhibit tumor growth, prevent metastasis, and increase the effectiveness of chemotherapy and radiotherapy. However, these improvements present difficulties such as isolation, scalability, and stability of delivered miRNA during storage.Furthermore, the issues related to off-target effects, as well as immunogenicity, can be a focus. The mechanisms of miRNA loading into MSC-EVs, as well as their targeting efficiency and therapeutic potential, can be outlined in this manuscript. For the final part of the manuscript, the current advances in MSC-EV engineering and potential strategies for clinical application have been described. The findings of MSC-EVs imply that they present MSC-EVs as a second-generation tool for precise oncology. |
| format | Article |
| id | doaj-art-6d2bf84eac4f488db111b496c5c03e93 |
| institution | OA Journals |
| issn | 2352-3204 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Regenerative Therapy |
| spelling | doaj-art-6d2bf84eac4f488db111b496c5c03e932025-08-20T01:57:19ZengElsevierRegenerative Therapy2352-32042025-03-012855857210.1016/j.reth.2025.01.019Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cellsAshok Kumar Balaraman0M. Arockia Babu1Muhammad Afzal2Gaurav Sanghvi3Rekha M M4Sofia Gupta5Mohit Rana6Haider Ali7Kavita Goyal8Vetriselvan Subramaniyan9Ling Shing Wong10Vinoth Kumarasamy11Research and Enterprise, University of Cyberjaya, Persiaran Bestari, Cyber 11, Cyberjaya, Selangor, 63000, MalaysiaInstitute of Pharmaceutical Research, GLA UNIVERSITY, Mathura, UP, 281406, IndiaDepartment of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, P.O. Box 6231, Jeddah, 21442, Saudi ArabiaMarwadi University Research Center, Department of Microbiology, Faculty of Science, Marwadi University, Rajkot, 360003, Gujarat, IndiaDepartment of Chemistry and Biochemistry, School of Sciences, JAIN (Deemed to be University), Bangalore, Karnataka, IndiaDepartment of Chemistry, Chandigarh Engineering College, Chandigarh Group of Colleges-Jhanjeri, Mohali, 140307, Punjab, IndiaUttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun, IndiaCentre for Global Health Research, Saveetha Medical College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, IndiaDepartment of Biotechnology, Graphic Era (Deemed to be University), Clement Town, 248002, Dehradun, IndiaDivision of Pharmacology, School of Medical and Life Sciences, Sunway University Jalan Universiti, Bandar Sunway, 47500 Selangor Darul Ehsan, MalaysiaFaculty of Health and Life Sciences, INTI International University, Nilai, 71800, MalaysiaDepartment of Parasitology, Medical Entomology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Jalan Yaacob Latif, 56000 Cheras, Kuala Lumpur, Malaysia; Corresponding author.Recently, increasing interest has been in utilizing mesenchymal stem cell-derived extracellular vesicles (MSC-EVs), especially exosomes, as nanocarriers for miRNA delivery in cancer treatment. Due to such characteristics, nanocarriers are specific: biocompatible, low immunogenicity, and capable of spontaneous tumor accumulation. MSC-EVs were loaded with therapeutic miRNAs and minimized their susceptibility to degradation by protecting the miRNA from accessibility to degrading enzymes and providing targeted delivery of the miRNAs to the tumor cells to modulate oncogenic pathways. In vitro and in vivo experiments suggest that MSC-EVs loaded with miRNAs may inhibit tumor growth, prevent metastasis, and increase the effectiveness of chemotherapy and radiotherapy. However, these improvements present difficulties such as isolation, scalability, and stability of delivered miRNA during storage.Furthermore, the issues related to off-target effects, as well as immunogenicity, can be a focus. The mechanisms of miRNA loading into MSC-EVs, as well as their targeting efficiency and therapeutic potential, can be outlined in this manuscript. For the final part of the manuscript, the current advances in MSC-EV engineering and potential strategies for clinical application have been described. The findings of MSC-EVs imply that they present MSC-EVs as a second-generation tool for precise oncology.http://www.sciencedirect.com/science/article/pii/S2352320425000197Cancer therapyMesenchymal stem cellsExtracellular vesiclesMicroRNA deliveryTargeted therapy |
| spellingShingle | Ashok Kumar Balaraman M. Arockia Babu Muhammad Afzal Gaurav Sanghvi Rekha M M Sofia Gupta Mohit Rana Haider Ali Kavita Goyal Vetriselvan Subramaniyan Ling Shing Wong Vinoth Kumarasamy Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cells Regenerative Therapy Cancer therapy Mesenchymal stem cells Extracellular vesicles MicroRNA delivery Targeted therapy |
| title | Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cells |
| title_full | Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cells |
| title_fullStr | Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cells |
| title_full_unstemmed | Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cells |
| title_short | Exosome-based miRNA delivery: Transforming cancer treatment with mesenchymal stem cells |
| title_sort | exosome based mirna delivery transforming cancer treatment with mesenchymal stem cells |
| topic | Cancer therapy Mesenchymal stem cells Extracellular vesicles MicroRNA delivery Targeted therapy |
| url | http://www.sciencedirect.com/science/article/pii/S2352320425000197 |
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