Development of an Integrated Multifunctional Column for Rapid Pretreatment and Determination of Trichothecenes in Cereals and Feeds with HPLC-MS/MS
The frequent detection of trichothecenes in grains highlights critical health risks to humans and animals. Based on the hybrid sorbent strategies, this study developed an innovative multifunctional column (ASAG563) integrating extraction, purification, and filtration to address limitations of existi...
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| Language: | English |
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MDPI AG
2025-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/9/1466 |
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| author | Sisi Liu Yu Wu Tongtong Liu Jin Ye Li Li Xiao Guan Songxue Wang |
| author_facet | Sisi Liu Yu Wu Tongtong Liu Jin Ye Li Li Xiao Guan Songxue Wang |
| author_sort | Sisi Liu |
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| description | The frequent detection of trichothecenes in grains highlights critical health risks to humans and animals. Based on the hybrid sorbent strategies, this study developed an innovative multifunctional column (ASAG563) integrating extraction, purification, and filtration to address limitations of existing methods, including cumbersome process, protracted duration, harmful to the environment, and significant matrix interference. Coupled with high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS), the ASAG563 column demonstrated superior recoveries (80.8–117.8%) and quantification limits (2.02~48.41 µg/kg) across cereals and feeds, with low relative standard deviations (<6.8%). Compared to commercial MFCs, the ASAG563 column simplified the process, reduced material consumption, saved 50% of analysis time, and effectively eliminated matrix effects. Analysis of 512 maize for feedstuff samples from Northeast China revealed significant contaminations with deoxynivalenol (DON) and its derivatives, emphasizing the necessity for enhanced regulatory measures. This novel integrated multifunctional pretreatment column presents a convenient, cost-effective, and eco-friendly solution for accurate TCT detection, significantly advancing analytical capabilities. |
| format | Article |
| id | doaj-art-6d26fe261d904b259a7d7fc9038cfe02 |
| institution | OA Journals |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-04-01 |
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| series | Foods |
| spelling | doaj-art-6d26fe261d904b259a7d7fc9038cfe022025-08-20T01:49:50ZengMDPI AGFoods2304-81582025-04-01149146610.3390/foods14091466Development of an Integrated Multifunctional Column for Rapid Pretreatment and Determination of Trichothecenes in Cereals and Feeds with HPLC-MS/MSSisi Liu0Yu Wu1Tongtong Liu2Jin Ye3Li Li4Xiao Guan5Songxue Wang6School of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaNFSRA Key Laboratory of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaNFSRA Key Laboratory of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaNFSRA Key Laboratory of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaNFSRA Key Laboratory of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaSchool of Health Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, ChinaNFSRA Key Laboratory of Grain and Oil Quality and Safety, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaThe frequent detection of trichothecenes in grains highlights critical health risks to humans and animals. Based on the hybrid sorbent strategies, this study developed an innovative multifunctional column (ASAG563) integrating extraction, purification, and filtration to address limitations of existing methods, including cumbersome process, protracted duration, harmful to the environment, and significant matrix interference. Coupled with high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS), the ASAG563 column demonstrated superior recoveries (80.8–117.8%) and quantification limits (2.02~48.41 µg/kg) across cereals and feeds, with low relative standard deviations (<6.8%). Compared to commercial MFCs, the ASAG563 column simplified the process, reduced material consumption, saved 50% of analysis time, and effectively eliminated matrix effects. Analysis of 512 maize for feedstuff samples from Northeast China revealed significant contaminations with deoxynivalenol (DON) and its derivatives, emphasizing the necessity for enhanced regulatory measures. This novel integrated multifunctional pretreatment column presents a convenient, cost-effective, and eco-friendly solution for accurate TCT detection, significantly advancing analytical capabilities.https://www.mdpi.com/2304-8158/14/9/1466multifunctional columntrichothecenesmycotoxinsHPLC-MS/MScerealsfeeds |
| spellingShingle | Sisi Liu Yu Wu Tongtong Liu Jin Ye Li Li Xiao Guan Songxue Wang Development of an Integrated Multifunctional Column for Rapid Pretreatment and Determination of Trichothecenes in Cereals and Feeds with HPLC-MS/MS Foods multifunctional column trichothecenes mycotoxins HPLC-MS/MS cereals feeds |
| title | Development of an Integrated Multifunctional Column for Rapid Pretreatment and Determination of Trichothecenes in Cereals and Feeds with HPLC-MS/MS |
| title_full | Development of an Integrated Multifunctional Column for Rapid Pretreatment and Determination of Trichothecenes in Cereals and Feeds with HPLC-MS/MS |
| title_fullStr | Development of an Integrated Multifunctional Column for Rapid Pretreatment and Determination of Trichothecenes in Cereals and Feeds with HPLC-MS/MS |
| title_full_unstemmed | Development of an Integrated Multifunctional Column for Rapid Pretreatment and Determination of Trichothecenes in Cereals and Feeds with HPLC-MS/MS |
| title_short | Development of an Integrated Multifunctional Column for Rapid Pretreatment and Determination of Trichothecenes in Cereals and Feeds with HPLC-MS/MS |
| title_sort | development of an integrated multifunctional column for rapid pretreatment and determination of trichothecenes in cereals and feeds with hplc ms ms |
| topic | multifunctional column trichothecenes mycotoxins HPLC-MS/MS cereals feeds |
| url | https://www.mdpi.com/2304-8158/14/9/1466 |
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