Correlation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking model
Abstract By investigating the correlation between the injection rate and pressure of subretinal tissue plasminogen activator (tPA) and air using a standard Viscous Fluid Control (VFC) system with a 38-gauge cannula, we aimed to establish guidelines for stable injections. We fabricated a retina mimic...
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Nature Portfolio
2024-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-77518-z |
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| author | Na Hee Kim Minsub Lee Hyewon Chung Hyung Chan Kim Hyungwoo Lee |
| author_facet | Na Hee Kim Minsub Lee Hyewon Chung Hyung Chan Kim Hyungwoo Lee |
| author_sort | Na Hee Kim |
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| description | Abstract By investigating the correlation between the injection rate and pressure of subretinal tissue plasminogen activator (tPA) and air using a standard Viscous Fluid Control (VFC) system with a 38-gauge cannula, we aimed to establish guidelines for stable injections. We fabricated a retina mimicking model (RMM) with 0.25% agarose solution and an aluminum plate, and substituted submacular hemorrhage (SMH) and tPA with blood-mimicking fluid (BMF) and balanced salt solution (BSS), respectively. The diameter of the pre-bleb mimicking SMH in RMM was 1.30 ± 0.16 cm, increasing to 1.98 ± 0.24 cm and 1.83 ± 0.22 cm after bleb propagation with BSS and air, respectively. BSS injection rates were 2.86 ± 0.04 µl/sec, 6.74 ± 0.48 µl/sec and 8.55 ± 0.16 µl/sec at 8, 12, and 16 psi, respectively. Air injection rates were 37.98 ± 3.11 µl/sec, 79.01 ± 5.13 µl/sec and 156.06 ± 13.72 µl/sec at 2, 3 and 4 psi, respectively. By experimenting with different pressures in the RMM, we found 12 psi to be the minimum for proper BSS injection and 2 psi for air. These findings provide crucial parameters for safer surgery to prevent irreversible damage. |
| format | Article |
| id | doaj-art-6d19710ffd2546afb6be7d9fa310803f |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-6d19710ffd2546afb6be7d9fa310803f2025-08-20T02:18:35ZengNature PortfolioScientific Reports2045-23222024-10-011411810.1038/s41598-024-77518-zCorrelation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking modelNa Hee Kim0Minsub Lee1Hyewon Chung2Hyung Chan Kim3Hyungwoo Lee4Department of Ophthalmology, College of Medicine, Konkuk University, Konkuk University Medical CenterDepartment of Ophthalmology, College of Medicine, Konkuk University, Konkuk University Medical CenterDepartment of Ophthalmology, College of Medicine, Konkuk University, Konkuk University Medical CenterKong Eye HospitalDepartment of Ophthalmology, College of Medicine, Konkuk University, Konkuk University Medical CenterAbstract By investigating the correlation between the injection rate and pressure of subretinal tissue plasminogen activator (tPA) and air using a standard Viscous Fluid Control (VFC) system with a 38-gauge cannula, we aimed to establish guidelines for stable injections. We fabricated a retina mimicking model (RMM) with 0.25% agarose solution and an aluminum plate, and substituted submacular hemorrhage (SMH) and tPA with blood-mimicking fluid (BMF) and balanced salt solution (BSS), respectively. The diameter of the pre-bleb mimicking SMH in RMM was 1.30 ± 0.16 cm, increasing to 1.98 ± 0.24 cm and 1.83 ± 0.22 cm after bleb propagation with BSS and air, respectively. BSS injection rates were 2.86 ± 0.04 µl/sec, 6.74 ± 0.48 µl/sec and 8.55 ± 0.16 µl/sec at 8, 12, and 16 psi, respectively. Air injection rates were 37.98 ± 3.11 µl/sec, 79.01 ± 5.13 µl/sec and 156.06 ± 13.72 µl/sec at 2, 3 and 4 psi, respectively. By experimenting with different pressures in the RMM, we found 12 psi to be the minimum for proper BSS injection and 2 psi for air. These findings provide crucial parameters for safer surgery to prevent irreversible damage.https://doi.org/10.1038/s41598-024-77518-z |
| spellingShingle | Na Hee Kim Minsub Lee Hyewon Chung Hyung Chan Kim Hyungwoo Lee Correlation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking model Scientific Reports |
| title | Correlation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking model |
| title_full | Correlation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking model |
| title_fullStr | Correlation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking model |
| title_full_unstemmed | Correlation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking model |
| title_short | Correlation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking model |
| title_sort | correlation between subretinal tissue plasminogen activator and air injection rates with pressure in a retina mimicking model |
| url | https://doi.org/10.1038/s41598-024-77518-z |
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