Online determination of phycocyanin in vivo in Galdieria sulphuraria
The extremophilic red alga Galdieria sulphuraria is well known for its metabolic capabilities with respect to organic carbon sources as well as its ability to produce the blue pigment phycocyanin (PC). The interest in PC as natural food colourant as well as nutraceutical is growing steadily. Therefo...
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Taylor & Francis Group
2024-12-01
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| Series: | Applied Phycology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/26388081.2024.2397702 |
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| author | Philipp Scherhag Michael Katzberg |
| author_facet | Philipp Scherhag Michael Katzberg |
| author_sort | Philipp Scherhag |
| collection | DOAJ |
| description | The extremophilic red alga Galdieria sulphuraria is well known for its metabolic capabilities with respect to organic carbon sources as well as its ability to produce the blue pigment phycocyanin (PC). The interest in PC as natural food colourant as well as nutraceutical is growing steadily. Therefore, a reliable quantification of PC accumulated by G. sulphuraria is crucial for establishing a production process. PC can be quantified through photometry but only after a laborious extraction procedure, which reduces the amount of data that can be gained from a cultivation. Hence, a faster PC determination method, with the potential to become an online method, is needed. In this study, we present a fast method for estimating the specific PC concentration based on in vivo absorbance spectra from cells of G. sulphuraria. A correlation with the PC concentration in the raw extract could be obtained, resulting in a 10-fold cross-validated coefficient of determination cR2 = 0.951. This makes extraction unnecessary in most cases. Especially in small-scale cultivation, where usually the available amount of biomass limits the number of measurements, this method presents a viable alternative to the extraction of PC. |
| format | Article |
| id | doaj-art-6d0f9ffaf9324c6591c96c7f8fc016f9 |
| institution | DOAJ |
| issn | 2638-8081 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Applied Phycology |
| spelling | doaj-art-6d0f9ffaf9324c6591c96c7f8fc016f92025-08-20T02:48:43ZengTaylor & Francis GroupApplied Phycology2638-80812024-12-0151728410.1080/26388081.2024.2397702Online determination of phycocyanin in vivo in Galdieria sulphurariaPhilipp Scherhag0Michael Katzberg1Department of Chemical Engineering, Chair of Bioprocess Engineering and Technical Biochemistry, University of Applied Sciences Dresden, Dresden, GermanyDepartment of Chemical Engineering, Chair of Bioprocess Engineering and Technical Biochemistry, University of Applied Sciences Dresden, Dresden, GermanyThe extremophilic red alga Galdieria sulphuraria is well known for its metabolic capabilities with respect to organic carbon sources as well as its ability to produce the blue pigment phycocyanin (PC). The interest in PC as natural food colourant as well as nutraceutical is growing steadily. Therefore, a reliable quantification of PC accumulated by G. sulphuraria is crucial for establishing a production process. PC can be quantified through photometry but only after a laborious extraction procedure, which reduces the amount of data that can be gained from a cultivation. Hence, a faster PC determination method, with the potential to become an online method, is needed. In this study, we present a fast method for estimating the specific PC concentration based on in vivo absorbance spectra from cells of G. sulphuraria. A correlation with the PC concentration in the raw extract could be obtained, resulting in a 10-fold cross-validated coefficient of determination cR2 = 0.951. This makes extraction unnecessary in most cases. Especially in small-scale cultivation, where usually the available amount of biomass limits the number of measurements, this method presents a viable alternative to the extraction of PC.https://www.tandfonline.com/doi/10.1080/26388081.2024.2397702Design of experimentextractionGaldieria sulphurariain vivo absorbancein vivo quantificationphycocyanin |
| spellingShingle | Philipp Scherhag Michael Katzberg Online determination of phycocyanin in vivo in Galdieria sulphuraria Applied Phycology Design of experiment extraction Galdieria sulphuraria in vivo absorbance in vivo quantification phycocyanin |
| title | Online determination of phycocyanin in vivo in Galdieria sulphuraria |
| title_full | Online determination of phycocyanin in vivo in Galdieria sulphuraria |
| title_fullStr | Online determination of phycocyanin in vivo in Galdieria sulphuraria |
| title_full_unstemmed | Online determination of phycocyanin in vivo in Galdieria sulphuraria |
| title_short | Online determination of phycocyanin in vivo in Galdieria sulphuraria |
| title_sort | online determination of phycocyanin in vivo in galdieria sulphuraria |
| topic | Design of experiment extraction Galdieria sulphuraria in vivo absorbance in vivo quantification phycocyanin |
| url | https://www.tandfonline.com/doi/10.1080/26388081.2024.2397702 |
| work_keys_str_mv | AT philippscherhag onlinedeterminationofphycocyanininvivoingaldieriasulphuraria AT michaelkatzberg onlinedeterminationofphycocyanininvivoingaldieriasulphuraria |