A high quality genome of the common swamp pitcher plant (Nepenthes mirabilis) using PacBio HiFi sequencing.
The genus Nepenthes, known commonly as tropical pitcher plants, are amongst the most recognisable carnivorous plants, capturing and digesting organic material in highly modified 'pitcher' leaves to acquire nitrogen and other valuable nutrients that are naturally scarce in their habitats. H...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0322885 |
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| author | Christopher J Jackson Todd G B McLay Gareth D Holmes Theodore R Allnutt Alastair S Robinson |
| author_facet | Christopher J Jackson Todd G B McLay Gareth D Holmes Theodore R Allnutt Alastair S Robinson |
| author_sort | Christopher J Jackson |
| collection | DOAJ |
| description | The genus Nepenthes, known commonly as tropical pitcher plants, are amongst the most recognisable carnivorous plants, capturing and digesting organic material in highly modified 'pitcher' leaves to acquire nitrogen and other valuable nutrients that are naturally scarce in their habitats. Here, PacBio HiFi long-read sequencing was used to assemble a near chromosome-level genome for a female specimen of Nepenthes mirabilis. We compare the genome organisation and gene content with a recently published chromosome-level assembly from a male specimen of the closely related N. gracilis, generated using Oxford Nanopore long-read sequencing and Hi-C scaffolding. We identify putative centromeres and large repeats in some N. mirabilis chromosomes that are absent from the N. gracilis assembly and examine the differences between the X and Y chromosomes, identifying a massive nuclear ribosomal repeat array in the N. mirabilis X-chromosome. |
| format | Article |
| id | doaj-art-6d00f56be932459ebbd9b2d80899c243 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-6d00f56be932459ebbd9b2d80899c2432025-08-20T02:40:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01207e032288510.1371/journal.pone.0322885A high quality genome of the common swamp pitcher plant (Nepenthes mirabilis) using PacBio HiFi sequencing.Christopher J JacksonTodd G B McLayGareth D HolmesTheodore R AllnuttAlastair S RobinsonThe genus Nepenthes, known commonly as tropical pitcher plants, are amongst the most recognisable carnivorous plants, capturing and digesting organic material in highly modified 'pitcher' leaves to acquire nitrogen and other valuable nutrients that are naturally scarce in their habitats. Here, PacBio HiFi long-read sequencing was used to assemble a near chromosome-level genome for a female specimen of Nepenthes mirabilis. We compare the genome organisation and gene content with a recently published chromosome-level assembly from a male specimen of the closely related N. gracilis, generated using Oxford Nanopore long-read sequencing and Hi-C scaffolding. We identify putative centromeres and large repeats in some N. mirabilis chromosomes that are absent from the N. gracilis assembly and examine the differences between the X and Y chromosomes, identifying a massive nuclear ribosomal repeat array in the N. mirabilis X-chromosome.https://doi.org/10.1371/journal.pone.0322885 |
| spellingShingle | Christopher J Jackson Todd G B McLay Gareth D Holmes Theodore R Allnutt Alastair S Robinson A high quality genome of the common swamp pitcher plant (Nepenthes mirabilis) using PacBio HiFi sequencing. PLoS ONE |
| title | A high quality genome of the common swamp pitcher plant (Nepenthes mirabilis) using PacBio HiFi sequencing. |
| title_full | A high quality genome of the common swamp pitcher plant (Nepenthes mirabilis) using PacBio HiFi sequencing. |
| title_fullStr | A high quality genome of the common swamp pitcher plant (Nepenthes mirabilis) using PacBio HiFi sequencing. |
| title_full_unstemmed | A high quality genome of the common swamp pitcher plant (Nepenthes mirabilis) using PacBio HiFi sequencing. |
| title_short | A high quality genome of the common swamp pitcher plant (Nepenthes mirabilis) using PacBio HiFi sequencing. |
| title_sort | high quality genome of the common swamp pitcher plant nepenthes mirabilis using pacbio hifi sequencing |
| url | https://doi.org/10.1371/journal.pone.0322885 |
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