Colistin Resistance in WHO-Designated Global Priority Pathogens Isolated from Wastewater Effluents of Two Hospitals in Enugu Metropolis, South East Nigeria
Introduction: Hospitals are breeding grounds for multidrug-resistant (MDR) bacteria, posing treatment challenges and increasing the risk of spreading "superbugs." This study investigates the prevalence of colistinresistant bacteria, a last-resort antibiotic, in wastewater from tertiary...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2024-06-01
|
| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://jommid.pasteur.ac.ir/article-1-659-en.html |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Introduction: Hospitals are breeding grounds for multidrug-resistant
(MDR) bacteria, posing treatment challenges and increasing the risk of
spreading "superbugs." This study investigates the prevalence of colistinresistant
bacteria, a last-resort antibiotic, in wastewater from tertiary
hospitals in Enugu, Nigeria. Methods: Twenty wastewater samples were
collected over three months from two tertiary hospitals in Enugu. A
standardized protocol by the American Public Health Association (APHA)
was followed. Samples were collected aseptically from key drainage points
and transported to the lab within 2 hours. Bacteria were isolated using the
pour-plate method and characterized by morphological and biochemical
tests, including Catalase, Oxidase, and Glucose Fermentation. Antibiotic
susceptibility was assessed using Kirby-Bauer disc diffusion, and colistin
resistance was confirmed via broth microdilution. Multiplex PCR detected
mcr genes indicating plasmid-mediated resistance. Data were analyzed using
SPSS version 23 with Chi-Square and ANOVA tests at a significance level
of P < 0.05. Results: Gram-negative bacteria were isolated from 63.1% of
samples, with Klebsiella spp. being the most prevalent, accounting for
24.6%. Colistin resistance was phenotypically observed in E. coli (83%),
Klebsiella spp. (75%), and Pseudomonas aeruginosa (100%).
Genotypically, E. coli harbored mcr-1 (17%) and mcr-3 (83%), while all
Klebsiella and Pseudomonas isolates carried multiple mcr genes.
Additionally, these bacteria showed resistance to multiple antibiotics,
including Septrin, Gentamycin, and Ceftriaxone. Conclusion: The
significant presence of colistin-resistant bacteria, especially E. coli and
Klebsiella, poses a public health concern, potentially leading to treatment
failures and spreading resistance genes. Stricter monitoring of hospital
wastewater is necessary to identify emerging resistance trends and improve
antibiotic practices in hospitals. |
|---|---|
| ISSN: | 2345-5349 2345-5330 |