Exogenous Cytokinins and Auxins Affect Double Cropping in <i>Vitis vinifera</i> L. cv. ‘Ortrugo’ Grown in a Temperate Climate: Preliminary Results

The success of double cropping in <i>Vitis vinifera</i> L. cultivated in temperate climates relies on bud forcing efficiency, which requires the prompt unlocking of apical dormant buds with sufficient fruitfulness. Chemical dormancy-breaking strategies need to be tested to enhance dorman...

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Main Authors: Filippo Del Zozzo, Harsh Tiwari, Ginevra Canavera, Tommaso Frioni, Stefano Poni
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Horticulturae
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Online Access:https://www.mdpi.com/2311-7524/11/4/346
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author Filippo Del Zozzo
Harsh Tiwari
Ginevra Canavera
Tommaso Frioni
Stefano Poni
author_facet Filippo Del Zozzo
Harsh Tiwari
Ginevra Canavera
Tommaso Frioni
Stefano Poni
author_sort Filippo Del Zozzo
collection DOAJ
description The success of double cropping in <i>Vitis vinifera</i> L. cultivated in temperate climates relies on bud forcing efficiency, which requires the prompt unlocking of apical dormant buds with sufficient fruitfulness. Chemical dormancy-breaking strategies need to be tested to enhance dormant bud forcing in summer pruning, as hydrogen cyanamide, the most used agent, could damage green organs. This study tested whether foliar applications of cytokinins and auxins could modulate dormancy release, potentially affecting bud forcing dynamics and shoot fruitfulness. The forcing treatments involved trimming primary shoots at the eighth node, removing lateral shoots, and retaining the main leaves and inflorescences. Five treatments were investigated: unforced control, control + 6-Benzyladenine application, forcing (FR), forcing + 6-Benzyladenine application (FBA), and forcing + Naphthaleneacetic acid application (FNAA). Phenological evolution, vegetative and productive parameters, and physiological characteristics have been assessed. Results showed that among the forcing treatments, FBA showed the highest forced/primary shoots ratio (106%), followed by FR (94%) and FNAA (21%). Primary yields were similar across treatments (2.74 kg), but total yield was highest in FBA (4.78 kg, including 2.02 kg from forced grapes), followed by FR (3.62 kg, with 1.09 kg forced). FNAA yielded no forced crop. During forced grapes maturation, photosynthesis rates were higher in forced leaves (11.1 μmol m<sup>−2</sup> s<sup>−1</sup>, as FR and FBA average) than primary leaves (−32%). Forced grapes ripened 47 days later and achieved higher sugar content (21.7 °Brix) and titratable acidity (10.6 g/L) than primary grapes. The findings suggest cytokinins application enhances bud forcing, supporting the feasibility of double cropping, while auxins limited it.
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spelling doaj-art-6ce8b71fc02341a8b2c81d4db03122d62025-08-20T02:28:36ZengMDPI AGHorticulturae2311-75242025-03-0111434610.3390/horticulturae11040346Exogenous Cytokinins and Auxins Affect Double Cropping in <i>Vitis vinifera</i> L. cv. ‘Ortrugo’ Grown in a Temperate Climate: Preliminary ResultsFilippo Del Zozzo0Harsh Tiwari1Ginevra Canavera2Tommaso Frioni3Stefano Poni4Department of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, ItalyDepartment of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, ItalyDepartment of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, ItalyDepartment of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, ItalyDepartment of Sustainable Crop Production, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, ItalyThe success of double cropping in <i>Vitis vinifera</i> L. cultivated in temperate climates relies on bud forcing efficiency, which requires the prompt unlocking of apical dormant buds with sufficient fruitfulness. Chemical dormancy-breaking strategies need to be tested to enhance dormant bud forcing in summer pruning, as hydrogen cyanamide, the most used agent, could damage green organs. This study tested whether foliar applications of cytokinins and auxins could modulate dormancy release, potentially affecting bud forcing dynamics and shoot fruitfulness. The forcing treatments involved trimming primary shoots at the eighth node, removing lateral shoots, and retaining the main leaves and inflorescences. Five treatments were investigated: unforced control, control + 6-Benzyladenine application, forcing (FR), forcing + 6-Benzyladenine application (FBA), and forcing + Naphthaleneacetic acid application (FNAA). Phenological evolution, vegetative and productive parameters, and physiological characteristics have been assessed. Results showed that among the forcing treatments, FBA showed the highest forced/primary shoots ratio (106%), followed by FR (94%) and FNAA (21%). Primary yields were similar across treatments (2.74 kg), but total yield was highest in FBA (4.78 kg, including 2.02 kg from forced grapes), followed by FR (3.62 kg, with 1.09 kg forced). FNAA yielded no forced crop. During forced grapes maturation, photosynthesis rates were higher in forced leaves (11.1 μmol m<sup>−2</sup> s<sup>−1</sup>, as FR and FBA average) than primary leaves (−32%). Forced grapes ripened 47 days later and achieved higher sugar content (21.7 °Brix) and titratable acidity (10.6 g/L) than primary grapes. The findings suggest cytokinins application enhances bud forcing, supporting the feasibility of double cropping, while auxins limited it.https://www.mdpi.com/2311-7524/11/4/346paradormancybud forcinggrapevineshoot fruitfulnessyieldfruitcomposition
spellingShingle Filippo Del Zozzo
Harsh Tiwari
Ginevra Canavera
Tommaso Frioni
Stefano Poni
Exogenous Cytokinins and Auxins Affect Double Cropping in <i>Vitis vinifera</i> L. cv. ‘Ortrugo’ Grown in a Temperate Climate: Preliminary Results
Horticulturae
paradormancy
bud forcing
grapevine
shoot fruitfulness
yield
fruitcomposition
title Exogenous Cytokinins and Auxins Affect Double Cropping in <i>Vitis vinifera</i> L. cv. ‘Ortrugo’ Grown in a Temperate Climate: Preliminary Results
title_full Exogenous Cytokinins and Auxins Affect Double Cropping in <i>Vitis vinifera</i> L. cv. ‘Ortrugo’ Grown in a Temperate Climate: Preliminary Results
title_fullStr Exogenous Cytokinins and Auxins Affect Double Cropping in <i>Vitis vinifera</i> L. cv. ‘Ortrugo’ Grown in a Temperate Climate: Preliminary Results
title_full_unstemmed Exogenous Cytokinins and Auxins Affect Double Cropping in <i>Vitis vinifera</i> L. cv. ‘Ortrugo’ Grown in a Temperate Climate: Preliminary Results
title_short Exogenous Cytokinins and Auxins Affect Double Cropping in <i>Vitis vinifera</i> L. cv. ‘Ortrugo’ Grown in a Temperate Climate: Preliminary Results
title_sort exogenous cytokinins and auxins affect double cropping in i vitis vinifera i l cv ortrugo grown in a temperate climate preliminary results
topic paradormancy
bud forcing
grapevine
shoot fruitfulness
yield
fruitcomposition
url https://www.mdpi.com/2311-7524/11/4/346
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