IL-9 and IL-10 Single-Nucleotide Variants and Serum Levels in Age-Related Macular Degeneration in the Caucasian Population
Considering the immunological impairment in age-related macular degeneration (AMD), we aimed to determine the associations of IL-9 rs1859430, rs2069870, rs11741137, rs2069885, and rs2069884 and IL-10 rs1800871, rs1800872, and rs1800896 polymorphisms and their haplotypes, as well as the serum levels...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/6622934 |
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| author | Alvita Vilkeviciute Dzastina Cebatoriene Loresa Kriauciuniene Reda Zemaitiene Rasa Liutkeviciene |
| author_facet | Alvita Vilkeviciute Dzastina Cebatoriene Loresa Kriauciuniene Reda Zemaitiene Rasa Liutkeviciene |
| author_sort | Alvita Vilkeviciute |
| collection | DOAJ |
| description | Considering the immunological impairment in age-related macular degeneration (AMD), we aimed to determine the associations of IL-9 rs1859430, rs2069870, rs11741137, rs2069885, and rs2069884 and IL-10 rs1800871, rs1800872, and rs1800896 polymorphisms and their haplotypes, as well as the serum levels of IL-9 and IL-10 with AMD. 1209 participants were enrolled in our study. SNPs were genotyped using TaqMan SNP genotyping assays by real-time PCR method. IL-9 and IL-10 serum levels were evaluated using ELISA kits. Our study results have shown that haplotypes A-G-C-G-G and G-A-T-A-T of IL-9 SNPs are associated with the decreased odds of early AMD occurrence (p=0.035 and p=0.015, respectively). A set of rare haplotypes was associated with the decreased odds of exudative AMD occurrence (p=0.033). Also, IL-10 serum levels were lower in exudative AMD than in controls (p=0.049), patients with early AMD (p=0.017), and atrophic AMD (p=0.008). Furthermore, exudative AMD patients with IL-10 rs1800896 CT and TT genotypes had lower IL-10 serum concentrations than those with wild-type (CC) genotype (p=0.048). In conclusion, our study suggests that IL-10 serum levels can be associated with a minor allele at IL-10 rs1800896 and exudative AMD. The haplotypes of IL-9 SNPs were also associated with the decreased odds of early and exudative AMD. |
| format | Article |
| id | doaj-art-6cda1fe8f41542d7894404202473cd8e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6cda1fe8f41542d7894404202473cd8e2025-08-20T03:34:10ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/66229346622934IL-9 and IL-10 Single-Nucleotide Variants and Serum Levels in Age-Related Macular Degeneration in the Caucasian PopulationAlvita Vilkeviciute0Dzastina Cebatoriene1Loresa Kriauciuniene2Reda Zemaitiene3Rasa Liutkeviciene4Neuroscience Institute, Lithuanian University of Health Sciences, Medical Academy, Eiveniu St. 2, Kaunas, LT-50161, LithuaniaDepartment of Ophthalmology, Lithuanian University of Health Sciences, Medical Academy, Eiveniu St. 2, Kaunas, LT-50161, LithuaniaNeuroscience Institute, Lithuanian University of Health Sciences, Medical Academy, Eiveniu St. 2, Kaunas, LT-50161, LithuaniaDepartment of Ophthalmology, Lithuanian University of Health Sciences, Medical Academy, Eiveniu St. 2, Kaunas, LT-50161, LithuaniaNeuroscience Institute, Lithuanian University of Health Sciences, Medical Academy, Eiveniu St. 2, Kaunas, LT-50161, LithuaniaConsidering the immunological impairment in age-related macular degeneration (AMD), we aimed to determine the associations of IL-9 rs1859430, rs2069870, rs11741137, rs2069885, and rs2069884 and IL-10 rs1800871, rs1800872, and rs1800896 polymorphisms and their haplotypes, as well as the serum levels of IL-9 and IL-10 with AMD. 1209 participants were enrolled in our study. SNPs were genotyped using TaqMan SNP genotyping assays by real-time PCR method. IL-9 and IL-10 serum levels were evaluated using ELISA kits. Our study results have shown that haplotypes A-G-C-G-G and G-A-T-A-T of IL-9 SNPs are associated with the decreased odds of early AMD occurrence (p=0.035 and p=0.015, respectively). A set of rare haplotypes was associated with the decreased odds of exudative AMD occurrence (p=0.033). Also, IL-10 serum levels were lower in exudative AMD than in controls (p=0.049), patients with early AMD (p=0.017), and atrophic AMD (p=0.008). Furthermore, exudative AMD patients with IL-10 rs1800896 CT and TT genotypes had lower IL-10 serum concentrations than those with wild-type (CC) genotype (p=0.048). In conclusion, our study suggests that IL-10 serum levels can be associated with a minor allele at IL-10 rs1800896 and exudative AMD. The haplotypes of IL-9 SNPs were also associated with the decreased odds of early and exudative AMD.http://dx.doi.org/10.1155/2021/6622934 |
| spellingShingle | Alvita Vilkeviciute Dzastina Cebatoriene Loresa Kriauciuniene Reda Zemaitiene Rasa Liutkeviciene IL-9 and IL-10 Single-Nucleotide Variants and Serum Levels in Age-Related Macular Degeneration in the Caucasian Population Mediators of Inflammation |
| title | IL-9 and IL-10 Single-Nucleotide Variants and Serum Levels in Age-Related Macular Degeneration in the Caucasian Population |
| title_full | IL-9 and IL-10 Single-Nucleotide Variants and Serum Levels in Age-Related Macular Degeneration in the Caucasian Population |
| title_fullStr | IL-9 and IL-10 Single-Nucleotide Variants and Serum Levels in Age-Related Macular Degeneration in the Caucasian Population |
| title_full_unstemmed | IL-9 and IL-10 Single-Nucleotide Variants and Serum Levels in Age-Related Macular Degeneration in the Caucasian Population |
| title_short | IL-9 and IL-10 Single-Nucleotide Variants and Serum Levels in Age-Related Macular Degeneration in the Caucasian Population |
| title_sort | il 9 and il 10 single nucleotide variants and serum levels in age related macular degeneration in the caucasian population |
| url | http://dx.doi.org/10.1155/2021/6622934 |
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