Evaluating the Impact of Human Amnion Epithelial Cells on Angiogenesis
The effects of human amnion epithelial cells (hAECs) on angiogenesis remain controversial. It is yet unknown if the presence of inflammation and/or gestational age of hAEC donors have an impact on angiogenesis. In this study, we examined the differences between term and preterm hAECs on angiogenesis...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/4565612 |
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| author | Dandan Zhu Ruth Muljadi Siow Teng Chan Patricia Vosdoganes Camden Lo Joanne C. Mockler Euan M. Wallace Rebecca Lim |
| author_facet | Dandan Zhu Ruth Muljadi Siow Teng Chan Patricia Vosdoganes Camden Lo Joanne C. Mockler Euan M. Wallace Rebecca Lim |
| author_sort | Dandan Zhu |
| collection | DOAJ |
| description | The effects of human amnion epithelial cells (hAECs) on angiogenesis remain controversial. It is yet unknown if the presence of inflammation and/or gestational age of hAEC donors have an impact on angiogenesis. In this study, we examined the differences between term and preterm hAECs on angiogenesis in vitro and in vivo. Conditioned media from term hAECs induced the formation of longer huVEC tubules on Matrigel. Both term and preterm hAECs expressed VEGFA, PDGFB, ANGPT1, and FOXC1, which significantly increased after TNFα and IFNγ stimulation. In the presence of TNFα and IFNγ, coculture with term hAECs reduced gene transcription of Tie-2 and Foxc1 in huVECs, while coculture with preterm hAECs increased gene transcription of PDGFRα and PDGFRβ and reduced gene transcription of FOXC1 in huVECs. In vivo assessment of angiogenesis using vWF immunostaining revealed that hAEC treatment decreased angiogenesis in a bleomycin model of lung fibrosis but increased angiogenesis in a neonatal model of hyperoxia-induced lung injury. In summary, our findings suggested that the impact of hAECs on angiogenesis may be influenced by the presence of inflammation and underlying pathology. |
| format | Article |
| id | doaj-art-6ccd140af6a948a19df68fec86ee7331 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-6ccd140af6a948a19df68fec86ee73312025-08-20T02:06:44ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/45656124565612Evaluating the Impact of Human Amnion Epithelial Cells on AngiogenesisDandan Zhu0Ruth Muljadi1Siow Teng Chan2Patricia Vosdoganes3Camden Lo4Joanne C. Mockler5Euan M. Wallace6Rebecca Lim7The Ritchie Centre, Hudson Institute of Medical Research, Monash University, Clayton, VIC, AustraliaThe Ritchie Centre, Hudson Institute of Medical Research, Monash University, Clayton, VIC, AustraliaThe Ritchie Centre, Hudson Institute of Medical Research, Monash University, Clayton, VIC, AustraliaDepartment of Obstetrics and Gynaecology, Monash University, Clayton, VIC, AustraliaMonash Microimaging, Monash University, Clayton, VIC, AustraliaThe Ritchie Centre, Hudson Institute of Medical Research, Monash University, Clayton, VIC, AustraliaThe Ritchie Centre, Hudson Institute of Medical Research, Monash University, Clayton, VIC, AustraliaThe Ritchie Centre, Hudson Institute of Medical Research, Monash University, Clayton, VIC, AustraliaThe effects of human amnion epithelial cells (hAECs) on angiogenesis remain controversial. It is yet unknown if the presence of inflammation and/or gestational age of hAEC donors have an impact on angiogenesis. In this study, we examined the differences between term and preterm hAECs on angiogenesis in vitro and in vivo. Conditioned media from term hAECs induced the formation of longer huVEC tubules on Matrigel. Both term and preterm hAECs expressed VEGFA, PDGFB, ANGPT1, and FOXC1, which significantly increased after TNFα and IFNγ stimulation. In the presence of TNFα and IFNγ, coculture with term hAECs reduced gene transcription of Tie-2 and Foxc1 in huVECs, while coculture with preterm hAECs increased gene transcription of PDGFRα and PDGFRβ and reduced gene transcription of FOXC1 in huVECs. In vivo assessment of angiogenesis using vWF immunostaining revealed that hAEC treatment decreased angiogenesis in a bleomycin model of lung fibrosis but increased angiogenesis in a neonatal model of hyperoxia-induced lung injury. In summary, our findings suggested that the impact of hAECs on angiogenesis may be influenced by the presence of inflammation and underlying pathology.http://dx.doi.org/10.1155/2016/4565612 |
| spellingShingle | Dandan Zhu Ruth Muljadi Siow Teng Chan Patricia Vosdoganes Camden Lo Joanne C. Mockler Euan M. Wallace Rebecca Lim Evaluating the Impact of Human Amnion Epithelial Cells on Angiogenesis Stem Cells International |
| title | Evaluating the Impact of Human Amnion Epithelial Cells on Angiogenesis |
| title_full | Evaluating the Impact of Human Amnion Epithelial Cells on Angiogenesis |
| title_fullStr | Evaluating the Impact of Human Amnion Epithelial Cells on Angiogenesis |
| title_full_unstemmed | Evaluating the Impact of Human Amnion Epithelial Cells on Angiogenesis |
| title_short | Evaluating the Impact of Human Amnion Epithelial Cells on Angiogenesis |
| title_sort | evaluating the impact of human amnion epithelial cells on angiogenesis |
| url | http://dx.doi.org/10.1155/2016/4565612 |
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