Loading scaffold-mediated multiple signal cycle for ultrasensitive microRNA analysis with low background signal
Abstract Alterations in microRNA (miRNA) expression profiles play a pivotal role in the initiation and progression of various diseases, including pediatric pneumonia. Consequently, the development of sensitive, specific, and precise methodologies for miRNA detection represents a promising tool for c...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2025-08-01
|
| Series: | Journal of Analytical Science and Technology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40543-025-00506-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849234908481847296 |
|---|---|
| author | Zhe Zhang Qiaoyi Xie Chenbo Zhu Huali Shao Boying Wu |
| author_facet | Zhe Zhang Qiaoyi Xie Chenbo Zhu Huali Shao Boying Wu |
| author_sort | Zhe Zhang |
| collection | DOAJ |
| description | Abstract Alterations in microRNA (miRNA) expression profiles play a pivotal role in the initiation and progression of various diseases, including pediatric pneumonia. Consequently, the development of sensitive, specific, and precise methodologies for miRNA detection represents a promising tool for clinical diagnosis and therapeutic intervention. This study proposes a highly sensitive miRNA detection approach utilizing catalytic hairpin assembly (CHA)-mediated probe release through chain extension and cascade signal amplification. The method enables precise discrimination of target miRNAs from other RNA species via a dual-target recognition mechanism. Furthermore, the incorporation of four signal amplification cycles confers substantial amplification efficiency, permitting accurate detection of low-abundance miRNAs. This system employs cascade isothermal amplification, demonstrating a linear response across concentrations from 1 fM to 100 pM and achieving a detection limit of 0.54 fM. The methodology exhibits significant potential for practical application in complex biological samples, providing a novel platform for profiling miRNA expression patterns and elucidating their mechanistic roles in disease pathogenesis. |
| format | Article |
| id | doaj-art-6ccbdf9ba7d94b8298be9dcd5bb7054c |
| institution | Kabale University |
| issn | 2093-3371 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj-art-6ccbdf9ba7d94b8298be9dcd5bb7054c2025-08-20T04:02:57ZengSpringerOpenJournal of Analytical Science and Technology2093-33712025-08-011611710.1186/s40543-025-00506-zLoading scaffold-mediated multiple signal cycle for ultrasensitive microRNA analysis with low background signalZhe Zhang0Qiaoyi Xie1Chenbo Zhu2Huali Shao3Boying Wu4Department of Pediatrics, Yinzhou District, The Affiliated People’s Hospital of Ningbo UniversityDepartment of Pediatrics, Yinzhou District, The Affiliated People’s Hospital of Ningbo UniversityDepartment of Pediatrics, Yinzhou District, The Affiliated People’s Hospital of Ningbo UniversityDepartment of Pediatrics, Yinzhou District, The Affiliated People’s Hospital of Ningbo UniversityDepartment of Pediatrics, Yinzhou District, The Affiliated People’s Hospital of Ningbo UniversityAbstract Alterations in microRNA (miRNA) expression profiles play a pivotal role in the initiation and progression of various diseases, including pediatric pneumonia. Consequently, the development of sensitive, specific, and precise methodologies for miRNA detection represents a promising tool for clinical diagnosis and therapeutic intervention. This study proposes a highly sensitive miRNA detection approach utilizing catalytic hairpin assembly (CHA)-mediated probe release through chain extension and cascade signal amplification. The method enables precise discrimination of target miRNAs from other RNA species via a dual-target recognition mechanism. Furthermore, the incorporation of four signal amplification cycles confers substantial amplification efficiency, permitting accurate detection of low-abundance miRNAs. This system employs cascade isothermal amplification, demonstrating a linear response across concentrations from 1 fM to 100 pM and achieving a detection limit of 0.54 fM. The methodology exhibits significant potential for practical application in complex biological samples, providing a novel platform for profiling miRNA expression patterns and elucidating their mechanistic roles in disease pathogenesis.https://doi.org/10.1186/s40543-025-00506-zMicroRNACatalytic hairpin assemblyDNA polymeraseEndonucleasePediatric pneumonia |
| spellingShingle | Zhe Zhang Qiaoyi Xie Chenbo Zhu Huali Shao Boying Wu Loading scaffold-mediated multiple signal cycle for ultrasensitive microRNA analysis with low background signal Journal of Analytical Science and Technology MicroRNA Catalytic hairpin assembly DNA polymerase Endonuclease Pediatric pneumonia |
| title | Loading scaffold-mediated multiple signal cycle for ultrasensitive microRNA analysis with low background signal |
| title_full | Loading scaffold-mediated multiple signal cycle for ultrasensitive microRNA analysis with low background signal |
| title_fullStr | Loading scaffold-mediated multiple signal cycle for ultrasensitive microRNA analysis with low background signal |
| title_full_unstemmed | Loading scaffold-mediated multiple signal cycle for ultrasensitive microRNA analysis with low background signal |
| title_short | Loading scaffold-mediated multiple signal cycle for ultrasensitive microRNA analysis with low background signal |
| title_sort | loading scaffold mediated multiple signal cycle for ultrasensitive microrna analysis with low background signal |
| topic | MicroRNA Catalytic hairpin assembly DNA polymerase Endonuclease Pediatric pneumonia |
| url | https://doi.org/10.1186/s40543-025-00506-z |
| work_keys_str_mv | AT zhezhang loadingscaffoldmediatedmultiplesignalcycleforultrasensitivemicrornaanalysiswithlowbackgroundsignal AT qiaoyixie loadingscaffoldmediatedmultiplesignalcycleforultrasensitivemicrornaanalysiswithlowbackgroundsignal AT chenbozhu loadingscaffoldmediatedmultiplesignalcycleforultrasensitivemicrornaanalysiswithlowbackgroundsignal AT hualishao loadingscaffoldmediatedmultiplesignalcycleforultrasensitivemicrornaanalysiswithlowbackgroundsignal AT boyingwu loadingscaffoldmediatedmultiplesignalcycleforultrasensitivemicrornaanalysiswithlowbackgroundsignal |