Evaluating a mustard extraction technique for sampling earthworms

ABSTRACT We examined efficacy of a mustard extraction technique for quantifying earthworm occupancy and density using a mesocosm experiment. In May 2017, we placed 12 earthworms from 2 ecological groups (6 surface dwelling [epigeic] and 6 deep dwelling [anecic]) into mesocosms (0.144 m2 surface area...

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Main Authors: Melissa D. Starking, Gary J. Roloff
Format: Article
Language:English
Published: Wiley 2019-12-01
Series:Wildlife Society Bulletin
Subjects:
Online Access:https://doi.org/10.1002/wsb.1033
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author Melissa D. Starking
Gary J. Roloff
author_facet Melissa D. Starking
Gary J. Roloff
author_sort Melissa D. Starking
collection DOAJ
description ABSTRACT We examined efficacy of a mustard extraction technique for quantifying earthworm occupancy and density using a mesocosm experiment. In May 2017, we placed 12 earthworms from 2 ecological groups (6 surface dwelling [epigeic] and 6 deep dwelling [anecic]) into mesocosms (0.144 m2 surface area) constructed from soils typically found in northern hardwood forests of Michigan, USA. We evaluated 2 soil depths (15 and 30 cm) and a soil moisture gradient (dry to wet). We found occupancy of epigeic earthworms was accurately portrayed 92% of the time through leaf searches, and subsequent mustard phase did not improve accuracy. For anecic earthworms, we found that occupancy was accurately portrayed 50% of the time through leaf searches and mustard phase increased accuracy to 92%. We found that mustard extraction produced 0–83% (median = 50%) of epigeic earthworms available for capture, with most recovered through leaf searches (median = 42%). We found that mustard extraction produced 0–67% (median = 33%) of anecic earthworms available for capture, with most (median = 20%) recovered during the mustard phase. Our results lend support for using mustard extraction as a technique for quantifying occupancy of epigeic and anecic earthworms, but caution that density estimates are likely low. © 2019 The Wildlife Society.
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spelling doaj-art-6cc394a4baf947af8afc64baa97bc2a02024-12-17T08:57:21ZengWileyWildlife Society Bulletin2328-55402019-12-0143476076510.1002/wsb.1033Evaluating a mustard extraction technique for sampling earthwormsMelissa D. Starking0Gary J. Roloff1Department of Fisheries and Wildlife Michigan State University 480 Wilson Road, Room 13, Natural Resources Building East Lansing MI 48824 USADepartment of Fisheries and Wildlife Michigan State University 480 Wilson Road, Room 13, Natural Resources Building East Lansing MI 48824 USAABSTRACT We examined efficacy of a mustard extraction technique for quantifying earthworm occupancy and density using a mesocosm experiment. In May 2017, we placed 12 earthworms from 2 ecological groups (6 surface dwelling [epigeic] and 6 deep dwelling [anecic]) into mesocosms (0.144 m2 surface area) constructed from soils typically found in northern hardwood forests of Michigan, USA. We evaluated 2 soil depths (15 and 30 cm) and a soil moisture gradient (dry to wet). We found occupancy of epigeic earthworms was accurately portrayed 92% of the time through leaf searches, and subsequent mustard phase did not improve accuracy. For anecic earthworms, we found that occupancy was accurately portrayed 50% of the time through leaf searches and mustard phase increased accuracy to 92%. We found that mustard extraction produced 0–83% (median = 50%) of epigeic earthworms available for capture, with most recovered through leaf searches (median = 42%). We found that mustard extraction produced 0–67% (median = 33%) of anecic earthworms available for capture, with most (median = 20%) recovered during the mustard phase. Our results lend support for using mustard extraction as a technique for quantifying occupancy of epigeic and anecic earthworms, but caution that density estimates are likely low. © 2019 The Wildlife Society.https://doi.org/10.1002/wsb.1033earthwormsMichiganmustard extractionnorthern hardwoods
spellingShingle Melissa D. Starking
Gary J. Roloff
Evaluating a mustard extraction technique for sampling earthworms
Wildlife Society Bulletin
earthworms
Michigan
mustard extraction
northern hardwoods
title Evaluating a mustard extraction technique for sampling earthworms
title_full Evaluating a mustard extraction technique for sampling earthworms
title_fullStr Evaluating a mustard extraction technique for sampling earthworms
title_full_unstemmed Evaluating a mustard extraction technique for sampling earthworms
title_short Evaluating a mustard extraction technique for sampling earthworms
title_sort evaluating a mustard extraction technique for sampling earthworms
topic earthworms
Michigan
mustard extraction
northern hardwoods
url https://doi.org/10.1002/wsb.1033
work_keys_str_mv AT melissadstarking evaluatingamustardextractiontechniqueforsamplingearthworms
AT garyjroloff evaluatingamustardextractiontechniqueforsamplingearthworms