Determination of 11 ginsenosides in ginseng health wine by HPLC
In this study, a high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 11 ginsenosides (ginsenoside Rg1, Re, Rf, Ra2, Rb1, Rc, Ra1, Ro, Rb2, Rb3, Rd) in ginseng health wine. Using Agilent SB C<sub>18</sub> column (4.6 mm×250...
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Editorial Department of China Brewing
2024-03-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-229.pdf |
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| author | HU Yang, LI Xianzhi, MAO Qiongli, LIU Yang, YAN Ling, ZHU Yan, SHI Hao |
| author_facet | HU Yang, LI Xianzhi, MAO Qiongli, LIU Yang, YAN Ling, ZHU Yan, SHI Hao |
| author_sort | HU Yang, LI Xianzhi, MAO Qiongli, LIU Yang, YAN Ling, ZHU Yan, SHI Hao |
| collection | DOAJ |
| description | In this study, a high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 11 ginsenosides (ginsenoside Rg1, Re, Rf, Ra2, Rb1, Rc, Ra1, Ro, Rb2, Rb3, Rd) in ginseng health wine. Using Agilent SB C<sub>18</sub> column (4.6 mm×250 mm, 5 μm), with acetonitrile-0.1% phosphoric acid as mobile phase gradient elution, flow rate 1.0 ml/min, column temperature 28 ℃, the detection wavelength of ginsenoside was 203 nm, and the injection volume was 10 μl. The results showed that 11 ginsenosides all had good linear relationship in their respective concentration range (R<sup>2</sup>≥0.999 9). The limits of detection were 1.52-3.03 mg/L, and the limits of quantitation were 4.59-9.18 mg/L. The relative standard deviation (RSD) of precision test, stability test and repetition test was less than 5%. The average recovery rate was 95.39%-101.96%, RSD was 0.07%-2.46%. The method had the advantages of simple operation, high accuracy and good repeatability, and could be used for the determination of 11 ginsenosides in ginseng health wine. |
| format | Article |
| id | doaj-art-6cc2b5bb874b431aad18920ca00ad9d4 |
| institution | DOAJ |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-6cc2b5bb874b431aad18920ca00ad9d42025-08-20T03:04:46ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-03-0143322923310.11882/j.issn.0254-5071.2024.03.037Determination of 11 ginsenosides in ginseng health wine by HPLCHU Yang, LI Xianzhi, MAO Qiongli, LIU Yang, YAN Ling, ZHU Yan, SHI Hao01. Jing Brand Co., Ltd., Huangshi 435100, China;;2. Hubei key Laboratory of Quality and Safety of Traditional Chinese Medicine Health Food, Jing Brand Co., Ltd., Huangshi 435100, ChinaIn this study, a high performance liquid chromatography (HPLC) method was established for the simultaneous determination of 11 ginsenosides (ginsenoside Rg1, Re, Rf, Ra2, Rb1, Rc, Ra1, Ro, Rb2, Rb3, Rd) in ginseng health wine. Using Agilent SB C<sub>18</sub> column (4.6 mm×250 mm, 5 μm), with acetonitrile-0.1% phosphoric acid as mobile phase gradient elution, flow rate 1.0 ml/min, column temperature 28 ℃, the detection wavelength of ginsenoside was 203 nm, and the injection volume was 10 μl. The results showed that 11 ginsenosides all had good linear relationship in their respective concentration range (R<sup>2</sup>≥0.999 9). The limits of detection were 1.52-3.03 mg/L, and the limits of quantitation were 4.59-9.18 mg/L. The relative standard deviation (RSD) of precision test, stability test and repetition test was less than 5%. The average recovery rate was 95.39%-101.96%, RSD was 0.07%-2.46%. The method had the advantages of simple operation, high accuracy and good repeatability, and could be used for the determination of 11 ginsenosides in ginseng health wine.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-229.pdfginseng health wine|ginsenoside|high performance liquid chromatography |
| spellingShingle | HU Yang, LI Xianzhi, MAO Qiongli, LIU Yang, YAN Ling, ZHU Yan, SHI Hao Determination of 11 ginsenosides in ginseng health wine by HPLC Zhongguo niangzao ginseng health wine|ginsenoside|high performance liquid chromatography |
| title | Determination of 11 ginsenosides in ginseng health wine by HPLC |
| title_full | Determination of 11 ginsenosides in ginseng health wine by HPLC |
| title_fullStr | Determination of 11 ginsenosides in ginseng health wine by HPLC |
| title_full_unstemmed | Determination of 11 ginsenosides in ginseng health wine by HPLC |
| title_short | Determination of 11 ginsenosides in ginseng health wine by HPLC |
| title_sort | determination of 11 ginsenosides in ginseng health wine by hplc |
| topic | ginseng health wine|ginsenoside|high performance liquid chromatography |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-3-229.pdf |
| work_keys_str_mv | AT huyanglixianzhimaoqiongliliuyangyanlingzhuyanshihao determinationof11ginsenosidesinginsenghealthwinebyhplc |