Dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspring
Summary: Dietary macronutrient composition in males influences brown adipose tissue (BAT) size and BAT size of daughters in C57BL/6J mice. However, the effects of macronutrients and paternal effects on BAT function have yet to be characterized. We investigated the effects of macronutrient compositio...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-08-01
|
| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725008216 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850072091070562304 |
|---|---|
| author | Erin L. Macartney Alistair M. Senior Angela J. Crean Lewin Small Lou Ruffino Tamara J. Pulpitel Marcelo A. Nobrega Romain Barrès Stephen J. Simpson |
| author_facet | Erin L. Macartney Alistair M. Senior Angela J. Crean Lewin Small Lou Ruffino Tamara J. Pulpitel Marcelo A. Nobrega Romain Barrès Stephen J. Simpson |
| author_sort | Erin L. Macartney |
| collection | DOAJ |
| description | Summary: Dietary macronutrient composition in males influences brown adipose tissue (BAT) size and BAT size of daughters in C57BL/6J mice. However, the effects of macronutrients and paternal effects on BAT function have yet to be characterized. We investigated the effects of macronutrient composition on the BAT proteome in male mice and offspring. In fathers, >50% of the proteome was affected by macronutrients. We identified two clusters with inverse patterns that correlated with BAT mass. Notably, uncoupling protein 1 (UCP1) was reduced on low-fat diets that promoted increased BAT mass, while there were increased levels of proteins involved in protein turnover. The same diets also led to a reduction in proteins involved in purine biosynthesis (purines are often UCP1 inhibitors). We also found that paternal protein intake negatively affected basigin expression in daughters, a protein that regulates Ucp1 transcription. Our results show that macronutrients in males remodel the protein expression of BAT directly and in their daughters. |
| format | Article |
| id | doaj-art-6cc12f8ee7444a279c30f566e1b7fa2a |
| institution | DOAJ |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-6cc12f8ee7444a279c30f566e1b7fa2a2025-08-20T02:47:09ZengElsevierCell Reports2211-12472025-08-0144811605010.1016/j.celrep.2025.116050Dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspringErin L. Macartney0Alistair M. Senior1Angela J. Crean2Lewin Small3Lou Ruffino4Tamara J. Pulpitel5Marcelo A. Nobrega6Romain Barrès7Stephen J. Simpson8Charles Perkins Centre, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia; Evolution and Ecology Research Centre, School of Biological, Earth, and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia; Institute of Organismic and Molecular Evolution, Johannes Gutenberg University of Mainz, 55128 Mainz, Germany; Corresponding authorCharles Perkins Centre, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, AustraliaCharles Perkins Centre, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, AustraliaCharles Perkins Centre, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, AustraliaCharles Perkins Centre, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia; Institut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur & Centre National pour la Recherche Scientifique (CNRS), 06560 Valbonne, FranceCharles Perkins Centre, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, AustraliaDepartment of Human Genetics, University of Chicago, Chicago, IL 60637, USAInstitut de Pharmacologie Moléculaire et Cellulaire, Université Côte d’Azur & Centre National pour la Recherche Scientifique (CNRS), 06560 Valbonne, France; Novo Nordisk Foundation Centre for Basic Metabolic Research, University of Copenhagen, Copenhagen 2200, DenmarkCharles Perkins Centre, School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, AustraliaSummary: Dietary macronutrient composition in males influences brown adipose tissue (BAT) size and BAT size of daughters in C57BL/6J mice. However, the effects of macronutrients and paternal effects on BAT function have yet to be characterized. We investigated the effects of macronutrient composition on the BAT proteome in male mice and offspring. In fathers, >50% of the proteome was affected by macronutrients. We identified two clusters with inverse patterns that correlated with BAT mass. Notably, uncoupling protein 1 (UCP1) was reduced on low-fat diets that promoted increased BAT mass, while there were increased levels of proteins involved in protein turnover. The same diets also led to a reduction in proteins involved in purine biosynthesis (purines are often UCP1 inhibitors). We also found that paternal protein intake negatively affected basigin expression in daughters, a protein that regulates Ucp1 transcription. Our results show that macronutrients in males remodel the protein expression of BAT directly and in their daughters.http://www.sciencedirect.com/science/article/pii/S2211124725008216CP: Metabolism |
| spellingShingle | Erin L. Macartney Alistair M. Senior Angela J. Crean Lewin Small Lou Ruffino Tamara J. Pulpitel Marcelo A. Nobrega Romain Barrès Stephen J. Simpson Dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspring Cell Reports CP: Metabolism |
| title | Dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspring |
| title_full | Dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspring |
| title_fullStr | Dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspring |
| title_full_unstemmed | Dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspring |
| title_short | Dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspring |
| title_sort | dietary macronutrients modulate the proteome of brown adipose tissue in males and their female offspring |
| topic | CP: Metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725008216 |
| work_keys_str_mv | AT erinlmacartney dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring AT alistairmsenior dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring AT angelajcrean dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring AT lewinsmall dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring AT louruffino dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring AT tamarajpulpitel dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring AT marceloanobrega dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring AT romainbarres dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring AT stephenjsimpson dietarymacronutrientsmodulatetheproteomeofbrownadiposetissueinmalesandtheirfemaleoffspring |