A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates
We present a method to synthesize mRNAs from synthetic DNA templates that produce biologically active proteins. To illustrate utility, we constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins. Examples include secreted lucifer...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-10-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/btn-2020-0037 |
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| author | John T Tossberg Tashawna M Esmond Thomas M Aune |
| author_facet | John T Tossberg Tashawna M Esmond Thomas M Aune |
| author_sort | John T Tossberg |
| collection | DOAJ |
| description | We present a method to synthesize mRNAs from synthetic DNA templates that produce biologically active proteins. To illustrate utility, we constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins. Examples include secreted luciferase, enhanced green fluorescence protein, IL-4, and IL-12A and IL-12B to form active IL-12. We propose that this method offers a cost- and time-saving alternative to plasmid-based cloning. |
| format | Article |
| id | doaj-art-6cbccd87a45f401fa875c41b0b7e5d4a |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-6cbccd87a45f401fa875c41b0b7e5d4a2025-08-20T02:26:02ZengTaylor & Francis GroupBioTechniques0736-62051940-98182020-10-0169428128810.2144/btn-2020-0037A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templatesJohn T Tossberg0Tashawna M Esmond1Thomas M Aune21Department of Medicine & Vanderbilt University Medical Center, Nashville, TN 37232, USA1Department of Medicine & Vanderbilt University Medical Center, Nashville, TN 37232, USA1Department of Medicine & Vanderbilt University Medical Center, Nashville, TN 37232, USAWe present a method to synthesize mRNAs from synthetic DNA templates that produce biologically active proteins. To illustrate utility, we constructed five unique synthetic DNA templates, produced mRNAs and demonstrated biologic activity of their translated proteins. Examples include secreted luciferase, enhanced green fluorescence protein, IL-4, and IL-12A and IL-12B to form active IL-12. We propose that this method offers a cost- and time-saving alternative to plasmid-based cloning.https://www.future-science.com/doi/10.2144/btn-2020-0037alternative to molecular cloningcell transfectionchemical DNA synthesisprotein translationsynthetic mRNA from DNA template |
| spellingShingle | John T Tossberg Tashawna M Esmond Thomas M Aune A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates BioTechniques alternative to molecular cloning cell transfection chemical DNA synthesis protein translation synthetic mRNA from DNA template |
| title | A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates |
| title_full | A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates |
| title_fullStr | A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates |
| title_full_unstemmed | A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates |
| title_short | A simplified method to produce mRNAs and functional proteins from synthetic double-stranded DNA templates |
| title_sort | simplified method to produce mrnas and functional proteins from synthetic double stranded dna templates |
| topic | alternative to molecular cloning cell transfection chemical DNA synthesis protein translation synthetic mRNA from DNA template |
| url | https://www.future-science.com/doi/10.2144/btn-2020-0037 |
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