Deciphering Planktonic Bacterial Community Assembly in the Storage Reservoir of the Long-Distance Water Diversion Project

Storage reservoirs are crucial components of long-distance water diversion projects, where water diversion may lead to changes in microbial diversity and community structure. Seasonal variations also drive alterations in microbial communities. However, the way that microbes assemble under the combin...

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Main Authors: Yingying Yang, Liguo Chen, Nianxin Wan, Ailing Xu, Ning Ding, Zhiwen Song
Format: Article
Language:English
Published: MDPI AG 2025-02-01
Series:Microorganisms
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Online Access:https://www.mdpi.com/2076-2607/13/2/465
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author Yingying Yang
Liguo Chen
Nianxin Wan
Ailing Xu
Ning Ding
Zhiwen Song
author_facet Yingying Yang
Liguo Chen
Nianxin Wan
Ailing Xu
Ning Ding
Zhiwen Song
author_sort Yingying Yang
collection DOAJ
description Storage reservoirs are crucial components of long-distance water diversion projects, where water diversion may lead to changes in microbial diversity and community structure. Seasonal variations also drive alterations in microbial communities. However, the way that microbes assemble under the combined effects of water diversion and seasonal variations in the storage reservoir has not been extensively studied. Jihongtan Reservoir is the terminal storage reservoir of the Yellow River to Qingdao Water Diversion Project (YQWD), which had an average annual water diversion period exceeding 290 days in recent years. In this study, 16S rDNA amplicon sequencing was used to investigate the seasonal dynamics and assembly of planktonic bacterial communities during the water diversion period in Jihongtan Reservoir. The results indicate that planktonic bacteria were able to maintain stable diversity across all four seasons, while the community structure underwent significant seasonal succession. Water temperature (WT) was found to be the primary driving environmental factor influencing the seasonal dynamic of planktonic bacterial communities. Co-occurrence network patterns of planktonic bacterial communities varied across different seasons, particularly in spring and winter. The spring network displayed the most complexity, showcasing the highest connectivity and greater stability. In contrast, the winter network was simpler, exhibiting lower local connectivity but higher global connectivity and lower stability. The analysis of the neutral community model and null model revealed that the relative importance of deterministic and stochastic processes in governing planktonic bacterial community assembly varies seasonally. Stochastic processes (dispersal limitation) are more prominent in spring, summer, and autumn, while deterministic processes (heterogeneous selection) play a greater role in winter. This study is essential for gaining a comprehensive understanding of the effects of water diversion projects and offers valuable references for the assessment of other similar projects.
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spelling doaj-art-6cbc80ce42654781af2e4c4da44b61d92025-08-20T03:12:01ZengMDPI AGMicroorganisms2076-26072025-02-0113246510.3390/microorganisms13020465Deciphering Planktonic Bacterial Community Assembly in the Storage Reservoir of the Long-Distance Water Diversion ProjectYingying Yang0Liguo Chen1Nianxin Wan2Ailing Xu3Ning Ding4Zhiwen Song5School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaShandong Water Transfer Project Operation and Maintenance Center, Jinan 250199, ChinaJihongtan Reservoir Management Station of Shandong Water Transfer Project Operation and Maintenance Center, Qingdao 266111, ChinaSchool of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaSchool of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaSchool of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, ChinaStorage reservoirs are crucial components of long-distance water diversion projects, where water diversion may lead to changes in microbial diversity and community structure. Seasonal variations also drive alterations in microbial communities. However, the way that microbes assemble under the combined effects of water diversion and seasonal variations in the storage reservoir has not been extensively studied. Jihongtan Reservoir is the terminal storage reservoir of the Yellow River to Qingdao Water Diversion Project (YQWD), which had an average annual water diversion period exceeding 290 days in recent years. In this study, 16S rDNA amplicon sequencing was used to investigate the seasonal dynamics and assembly of planktonic bacterial communities during the water diversion period in Jihongtan Reservoir. The results indicate that planktonic bacteria were able to maintain stable diversity across all four seasons, while the community structure underwent significant seasonal succession. Water temperature (WT) was found to be the primary driving environmental factor influencing the seasonal dynamic of planktonic bacterial communities. Co-occurrence network patterns of planktonic bacterial communities varied across different seasons, particularly in spring and winter. The spring network displayed the most complexity, showcasing the highest connectivity and greater stability. In contrast, the winter network was simpler, exhibiting lower local connectivity but higher global connectivity and lower stability. The analysis of the neutral community model and null model revealed that the relative importance of deterministic and stochastic processes in governing planktonic bacterial community assembly varies seasonally. Stochastic processes (dispersal limitation) are more prominent in spring, summer, and autumn, while deterministic processes (heterogeneous selection) play a greater role in winter. This study is essential for gaining a comprehensive understanding of the effects of water diversion projects and offers valuable references for the assessment of other similar projects.https://www.mdpi.com/2076-2607/13/2/465co-occurrence network patternsenvironmental driving factorsplanktonic bacterial community assemblyseasonal dynamicsstorage reservoir
spellingShingle Yingying Yang
Liguo Chen
Nianxin Wan
Ailing Xu
Ning Ding
Zhiwen Song
Deciphering Planktonic Bacterial Community Assembly in the Storage Reservoir of the Long-Distance Water Diversion Project
Microorganisms
co-occurrence network patterns
environmental driving factors
planktonic bacterial community assembly
seasonal dynamics
storage reservoir
title Deciphering Planktonic Bacterial Community Assembly in the Storage Reservoir of the Long-Distance Water Diversion Project
title_full Deciphering Planktonic Bacterial Community Assembly in the Storage Reservoir of the Long-Distance Water Diversion Project
title_fullStr Deciphering Planktonic Bacterial Community Assembly in the Storage Reservoir of the Long-Distance Water Diversion Project
title_full_unstemmed Deciphering Planktonic Bacterial Community Assembly in the Storage Reservoir of the Long-Distance Water Diversion Project
title_short Deciphering Planktonic Bacterial Community Assembly in the Storage Reservoir of the Long-Distance Water Diversion Project
title_sort deciphering planktonic bacterial community assembly in the storage reservoir of the long distance water diversion project
topic co-occurrence network patterns
environmental driving factors
planktonic bacterial community assembly
seasonal dynamics
storage reservoir
url https://www.mdpi.com/2076-2607/13/2/465
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