Evaluation of the Effectiveness of a Matrix of Exogenous Proteases in the Nutrition of Shrimp <i>Penaeus vannamei</i>

This study evaluated the effectiveness of a matrix of acid and alkaline proteases in diets for <i>Penaeus vannamei</i> shrimp, considering zootechnical performance, meat yield, and intestinal histomorphometry. The experiment was carried out in 32 experimental units, with four treatments...

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Main Authors: Joice Teixeira Souza, Maria Érica da Silva Oliveira, Ana Elidarly da Cunha, Vanessa Maria Freitas da Silva, Ruan Arthur Nunes de Araújo, Mário Augusto Monteiro Silva, Raimundo Audei Henrique Júnior, Marcos Aurelio Victor de Assunção, Ana Cecília Araújo Lopes, Daniel Pigatto Monteiro, Thiago Pereira Ribeiro, Moacir Franco de Oliveira, Matheus Ramalho de Lima
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Animals
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Online Access:https://www.mdpi.com/2076-2615/15/10/1410
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author Joice Teixeira Souza
Maria Érica da Silva Oliveira
Ana Elidarly da Cunha
Vanessa Maria Freitas da Silva
Ruan Arthur Nunes de Araújo
Mário Augusto Monteiro Silva
Raimundo Audei Henrique Júnior
Marcos Aurelio Victor de Assunção
Ana Cecília Araújo Lopes
Daniel Pigatto Monteiro
Thiago Pereira Ribeiro
Moacir Franco de Oliveira
Matheus Ramalho de Lima
author_facet Joice Teixeira Souza
Maria Érica da Silva Oliveira
Ana Elidarly da Cunha
Vanessa Maria Freitas da Silva
Ruan Arthur Nunes de Araújo
Mário Augusto Monteiro Silva
Raimundo Audei Henrique Júnior
Marcos Aurelio Victor de Assunção
Ana Cecília Araújo Lopes
Daniel Pigatto Monteiro
Thiago Pereira Ribeiro
Moacir Franco de Oliveira
Matheus Ramalho de Lima
author_sort Joice Teixeira Souza
collection DOAJ
description This study evaluated the effectiveness of a matrix of acid and alkaline proteases in diets for <i>Penaeus vannamei</i> shrimp, considering zootechnical performance, meat yield, and intestinal histomorphometry. The experiment was carried out in 32 experimental units, with four treatments and eight replications each, using a density of 80 shrimp per square meter. The treatments consisted of positive control (PC) with standard diet, negative control (NC) with nutritional reduction, PC supplemented with 250 g/t of proteases (PCE), and NC supplemented with 250 g/t of proteases (NCE). In the fattening phase, significantly greater weight gain was observed in the NCE (14.23 g) and PCE (13.6 g) groups (<i>p</i> = 0.0381). Feed efficiency increased by 56.07% in the NCE group. Weekly growth was higher in the PCE (113.4%) and NCE (118.6%) groups (<i>p</i> = 0.028), with post-slaughter yields of 53.03% (NCE) and 52.21% (PCE). Water and humidity retention capacity remained stable in the NCE group during 210 days of frozen storage after slaughter. Intestinal histology indicated a greater absorption area in the groups with the best feed conversion and growth rates. It is concluded that protease supplementation improves nutrient absorption, growth, and meat yield in <i>P. vannamei.</i>
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spelling doaj-art-6cb053676f5c455d93e8fc499cbcd2222025-08-20T03:14:35ZengMDPI AGAnimals2076-26152025-05-011510141010.3390/ani15101410Evaluation of the Effectiveness of a Matrix of Exogenous Proteases in the Nutrition of Shrimp <i>Penaeus vannamei</i>Joice Teixeira Souza0Maria Érica da Silva Oliveira1Ana Elidarly da Cunha2Vanessa Maria Freitas da Silva3Ruan Arthur Nunes de Araújo4Mário Augusto Monteiro Silva5Raimundo Audei Henrique Júnior6Marcos Aurelio Victor de Assunção7Ana Cecília Araújo Lopes8Daniel Pigatto Monteiro9Thiago Pereira Ribeiro10Moacir Franco de Oliveira11Matheus Ramalho de Lima12Animal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilR&D in Nutrition at TECTRON, Toledo 85902-010, PR, BrazilR&D in Nutrition at TECTRON, Toledo 85902-010, PR, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilAnimal Science Department, Campus Mossoro, Federal Rural University of the Semi-Arid Region (UFERSA), Mossoro 59625-900, RN, BrazilThis study evaluated the effectiveness of a matrix of acid and alkaline proteases in diets for <i>Penaeus vannamei</i> shrimp, considering zootechnical performance, meat yield, and intestinal histomorphometry. The experiment was carried out in 32 experimental units, with four treatments and eight replications each, using a density of 80 shrimp per square meter. The treatments consisted of positive control (PC) with standard diet, negative control (NC) with nutritional reduction, PC supplemented with 250 g/t of proteases (PCE), and NC supplemented with 250 g/t of proteases (NCE). In the fattening phase, significantly greater weight gain was observed in the NCE (14.23 g) and PCE (13.6 g) groups (<i>p</i> = 0.0381). Feed efficiency increased by 56.07% in the NCE group. Weekly growth was higher in the PCE (113.4%) and NCE (118.6%) groups (<i>p</i> = 0.028), with post-slaughter yields of 53.03% (NCE) and 52.21% (PCE). Water and humidity retention capacity remained stable in the NCE group during 210 days of frozen storage after slaughter. Intestinal histology indicated a greater absorption area in the groups with the best feed conversion and growth rates. It is concluded that protease supplementation improves nutrient absorption, growth, and meat yield in <i>P. vannamei.</i>https://www.mdpi.com/2076-2615/15/10/1410shrimpexogenous enzymesproteaseperformancefish qualityhistology
spellingShingle Joice Teixeira Souza
Maria Érica da Silva Oliveira
Ana Elidarly da Cunha
Vanessa Maria Freitas da Silva
Ruan Arthur Nunes de Araújo
Mário Augusto Monteiro Silva
Raimundo Audei Henrique Júnior
Marcos Aurelio Victor de Assunção
Ana Cecília Araújo Lopes
Daniel Pigatto Monteiro
Thiago Pereira Ribeiro
Moacir Franco de Oliveira
Matheus Ramalho de Lima
Evaluation of the Effectiveness of a Matrix of Exogenous Proteases in the Nutrition of Shrimp <i>Penaeus vannamei</i>
Animals
shrimp
exogenous enzymes
protease
performance
fish quality
histology
title Evaluation of the Effectiveness of a Matrix of Exogenous Proteases in the Nutrition of Shrimp <i>Penaeus vannamei</i>
title_full Evaluation of the Effectiveness of a Matrix of Exogenous Proteases in the Nutrition of Shrimp <i>Penaeus vannamei</i>
title_fullStr Evaluation of the Effectiveness of a Matrix of Exogenous Proteases in the Nutrition of Shrimp <i>Penaeus vannamei</i>
title_full_unstemmed Evaluation of the Effectiveness of a Matrix of Exogenous Proteases in the Nutrition of Shrimp <i>Penaeus vannamei</i>
title_short Evaluation of the Effectiveness of a Matrix of Exogenous Proteases in the Nutrition of Shrimp <i>Penaeus vannamei</i>
title_sort evaluation of the effectiveness of a matrix of exogenous proteases in the nutrition of shrimp i penaeus vannamei i
topic shrimp
exogenous enzymes
protease
performance
fish quality
histology
url https://www.mdpi.com/2076-2615/15/10/1410
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