The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem
Summary: In the root meristem of Arabidopsis, stem cell maintenance depends on the coordinated action of transcription factor networks. The transcriptional regulator EMBRYO DEFECTIVE 1579 (EMB1579), a protein that forms nuclear condensates, regulates plant growth. However, the molecular mechanisms t...
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| Language: | English |
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Elsevier
2025-05-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124725004310 |
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| author | Fatimah Aljedaani Yinghui Luo Yanming Deng Wouter Smet Zeeshan Nasim Xinjing Xu Umar F. Shahul Hameed Ting Ting Xiao Jose Kenyi Gonzalez-Kise Stefan Arold Ikram Blilou |
| author_facet | Fatimah Aljedaani Yinghui Luo Yanming Deng Wouter Smet Zeeshan Nasim Xinjing Xu Umar F. Shahul Hameed Ting Ting Xiao Jose Kenyi Gonzalez-Kise Stefan Arold Ikram Blilou |
| author_sort | Fatimah Aljedaani |
| collection | DOAJ |
| description | Summary: In the root meristem of Arabidopsis, stem cell maintenance depends on the coordinated action of transcription factor networks. The transcriptional regulator EMBRYO DEFECTIVE 1579 (EMB1579), a protein that forms nuclear condensates, regulates plant growth. However, the molecular mechanisms through which it functions in the root meristem remain largely unclear. Here, we show that EMB1579 is required for stem cell maintenance and proper cell division orientation. EMB1579 modulates the function of two root stem cell regulatory modules, PLETHORAs and SCARECROW-SHORT-ROOT, through a process involving transcriptional regulation and RNA splicing. We show that EMB1579 acts as a catalyst for stem cell gene expression, and its activity is fine-tuned by its physical association with RNA splicing factors. The formation of nuclear condensates is essential for EMB1579 function in the root meristem. Our findings reveal a mechanism by which EMB1579 regulates stem cell determinants in the root meristem and expand the understanding of gene regulation complexity in plant development. |
| format | Article |
| id | doaj-art-6c9f01b413b64aefb60314c9562cd24d |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-6c9f01b413b64aefb60314c9562cd24d2025-08-20T03:52:43ZengElsevierCell Reports2211-12472025-05-0144511566010.1016/j.celrep.2025.115660The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristemFatimah Aljedaani0Yinghui Luo1Yanming Deng2Wouter Smet3Zeeshan Nasim4Xinjing Xu5Umar F. Shahul Hameed6Ting Ting Xiao7Jose Kenyi Gonzalez-Kise8Stefan Arold9Ikram Blilou10Plant Cell and Developmental Biology Laboratory, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaPlant Cell and Developmental Biology Laboratory, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaJiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Institute of Leisure Agriculture, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, ChinaPlant Cell and Developmental Biology Laboratory, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaDepartment of Life Sciences, Korea University, Seoul 02841, KoreaPlant Cell and Developmental Biology Laboratory, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaBiological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaPlant Cell and Developmental Biology Laboratory, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaPlant Cell and Developmental Biology Laboratory, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaBiological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi ArabiaPlant Cell and Developmental Biology Laboratory, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia; Corresponding authorSummary: In the root meristem of Arabidopsis, stem cell maintenance depends on the coordinated action of transcription factor networks. The transcriptional regulator EMBRYO DEFECTIVE 1579 (EMB1579), a protein that forms nuclear condensates, regulates plant growth. However, the molecular mechanisms through which it functions in the root meristem remain largely unclear. Here, we show that EMB1579 is required for stem cell maintenance and proper cell division orientation. EMB1579 modulates the function of two root stem cell regulatory modules, PLETHORAs and SCARECROW-SHORT-ROOT, through a process involving transcriptional regulation and RNA splicing. We show that EMB1579 acts as a catalyst for stem cell gene expression, and its activity is fine-tuned by its physical association with RNA splicing factors. The formation of nuclear condensates is essential for EMB1579 function in the root meristem. Our findings reveal a mechanism by which EMB1579 regulates stem cell determinants in the root meristem and expand the understanding of gene regulation complexity in plant development.http://www.sciencedirect.com/science/article/pii/S2211124725004310CP: Plants |
| spellingShingle | Fatimah Aljedaani Yinghui Luo Yanming Deng Wouter Smet Zeeshan Nasim Xinjing Xu Umar F. Shahul Hameed Ting Ting Xiao Jose Kenyi Gonzalez-Kise Stefan Arold Ikram Blilou The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem Cell Reports CP: Plants |
| title | The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem |
| title_full | The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem |
| title_fullStr | The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem |
| title_full_unstemmed | The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem |
| title_short | The dual function of EMB1579 in transcription and splicing governs tissue patterning in the Arabidopsis root meristem |
| title_sort | dual function of emb1579 in transcription and splicing governs tissue patterning in the arabidopsis root meristem |
| topic | CP: Plants |
| url | http://www.sciencedirect.com/science/article/pii/S2211124725004310 |
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