Purification and Characterization of a New Lectin from Loach Skin Mucus

Lectin from loach skin mucus plays an important role in pathogen defense. However, hardly can any paper relevant to the character of lectin from loach skin mucus be found in recent years. In this study, a kind of new lectin (LML), with a high hemagglutination activity of 166.23 × 103 HU/mg, was succ...

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Main Authors: Peng-peng Sun, Yuan-yuan Ren, Jie Zheng, Ai-jun Hu
Format: Article
Language:English
Published: Wiley 2019-01-01
Series:Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2019/3853646
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author Peng-peng Sun
Yuan-yuan Ren
Jie Zheng
Ai-jun Hu
author_facet Peng-peng Sun
Yuan-yuan Ren
Jie Zheng
Ai-jun Hu
author_sort Peng-peng Sun
collection DOAJ
description Lectin from loach skin mucus plays an important role in pathogen defense. However, hardly can any paper relevant to the character of lectin from loach skin mucus be found in recent years. In this study, a kind of new lectin (LML), with a high hemagglutination activity of 166.23 × 103 HU/mg, was successfully isolated and purified from loach skin mucus. LML was a kind of glycoprotein with a molecular weight of 245 kDa. Also, the monosaccharide composition suggested that its carbohydrate chain was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 2.02 : 11.66 : 2.06 : 1.00 : 14.09 : 6.00. Besides, LML depended on Ca2+ to induce hemagglutination and was strongly inhibited by D-lactose. The lectin exhibited powerful resistance to alkali and kept about 30% hemagglutination activity at pH 14.0, whereas its capacity of acid resistance was weak. The maximum hemagglutination activity of LML maintained at a temperature range from 20°C to 50°C. Moreover, the structure of LML was preliminarily studied, indicating it contained abundant glutamic acid, histidine, and serine, and its secondary structure contained α-helix (4.97%), β-sheet (27.55%), turns structure (49.78%), and unordered structure (17.70%).
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spelling doaj-art-6c9ea3891cf14be6a0eedd200cbd26322025-02-03T06:13:11ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/38536463853646Purification and Characterization of a New Lectin from Loach Skin MucusPeng-peng Sun0Yuan-yuan Ren1Jie Zheng2Ai-jun Hu3State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, Tianjin 300457, ChinaLectin from loach skin mucus plays an important role in pathogen defense. However, hardly can any paper relevant to the character of lectin from loach skin mucus be found in recent years. In this study, a kind of new lectin (LML), with a high hemagglutination activity of 166.23 × 103 HU/mg, was successfully isolated and purified from loach skin mucus. LML was a kind of glycoprotein with a molecular weight of 245 kDa. Also, the monosaccharide composition suggested that its carbohydrate chain was composed of rhamnose, arabinose, xylose, mannose, glucose, and galactose with a molar ratio of 2.02 : 11.66 : 2.06 : 1.00 : 14.09 : 6.00. Besides, LML depended on Ca2+ to induce hemagglutination and was strongly inhibited by D-lactose. The lectin exhibited powerful resistance to alkali and kept about 30% hemagglutination activity at pH 14.0, whereas its capacity of acid resistance was weak. The maximum hemagglutination activity of LML maintained at a temperature range from 20°C to 50°C. Moreover, the structure of LML was preliminarily studied, indicating it contained abundant glutamic acid, histidine, and serine, and its secondary structure contained α-helix (4.97%), β-sheet (27.55%), turns structure (49.78%), and unordered structure (17.70%).http://dx.doi.org/10.1155/2019/3853646
spellingShingle Peng-peng Sun
Yuan-yuan Ren
Jie Zheng
Ai-jun Hu
Purification and Characterization of a New Lectin from Loach Skin Mucus
Journal of Chemistry
title Purification and Characterization of a New Lectin from Loach Skin Mucus
title_full Purification and Characterization of a New Lectin from Loach Skin Mucus
title_fullStr Purification and Characterization of a New Lectin from Loach Skin Mucus
title_full_unstemmed Purification and Characterization of a New Lectin from Loach Skin Mucus
title_short Purification and Characterization of a New Lectin from Loach Skin Mucus
title_sort purification and characterization of a new lectin from loach skin mucus
url http://dx.doi.org/10.1155/2019/3853646
work_keys_str_mv AT pengpengsun purificationandcharacterizationofanewlectinfromloachskinmucus
AT yuanyuanren purificationandcharacterizationofanewlectinfromloachskinmucus
AT jiezheng purificationandcharacterizationofanewlectinfromloachskinmucus
AT aijunhu purificationandcharacterizationofanewlectinfromloachskinmucus