Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters
There are a number of compounds that can modify the activity of ABC (ATP-binding cassette) and SLC (solute carrier) transporters in the blood-brain barrier (BBB). The aim of this study was to investigate the effect of natural and synthetic substances on the expression level of genes encoding transpo...
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| Format: | Article |
| Language: | English |
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Sciendo
2014-06-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0020/acph-2014-0020.xml?format=INT |
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| author | Mrozikiewicz Przemyslaw M. Bogacz Anna Bartkowiak-Wieczorek Joanna Kujawski Radoslaw Mikolajczak Przemyslaw L. Ozarowski Marcin Czerny Boguslaw Mrozikiewicz-Rakowska Beata Grzeskowiak Edmund |
| author_facet | Mrozikiewicz Przemyslaw M. Bogacz Anna Bartkowiak-Wieczorek Joanna Kujawski Radoslaw Mikolajczak Przemyslaw L. Ozarowski Marcin Czerny Boguslaw Mrozikiewicz-Rakowska Beata Grzeskowiak Edmund |
| author_sort | Mrozikiewicz Przemyslaw M. |
| collection | DOAJ |
| description | There are a number of compounds that can modify the activity of ABC (ATP-binding cassette) and SLC (solute carrier) transporters in the blood-brain barrier (BBB). The aim of this study was to investigate the effect of natural and synthetic substances on the expression level of genes encoding transporters present in the BBB (mdr1a, mdr1b, mrp1, mrp2, oatp1a4, oatp1a5 and oatp1c1). Our results showed that verapamil caused the greatest reduction in the mRNA level while other synthetic (piracetam, phenobarbital) and natural (codeine, cyclosporine A, quercetin) substances showed a selective inhibitory effect. Further, the extract from the roots of Panax ginseng C. A. Meyer exhibited a decrease of transcription against selected transporters whereas the extract from Ginkgo biloba L. leaves resulted in an increase of the expression level of tested genes, except for mrp2. Extract from the aerial parts of Hypericum perforatum L. was the only one to cause an increased mRNA level for mdr1 and oatp1c1. These findings suggest that herbs can play an important role in overcoming the BBB and multidrug resistance to pharmacotherapy of brain cancer and mental disorders, based on the activity of selected drug-metabolizing enzymes and transporters located in the BBB |
| format | Article |
| id | doaj-art-6c97df45e8404e6f93b35433c48f1668 |
| institution | Kabale University |
| issn | 1330-0075 1846-9558 |
| language | English |
| publishDate | 2014-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-6c97df45e8404e6f93b35433c48f16682025-02-02T13:59:44ZengSciendoActa Pharmaceutica1330-00751846-95582014-06-0164222323210.2478/acph-2014-0020acph-2014-0020Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transportersMrozikiewicz Przemyslaw M.0Bogacz Anna1Bartkowiak-Wieczorek Joanna2Kujawski Radoslaw3Mikolajczak Przemyslaw L.4Ozarowski Marcin5Czerny Boguslaw6Mrozikiewicz-Rakowska Beata7Grzeskowiak Edmund8Laboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, 14 Marii Magdaleny Street, 61-861 Poznan, PolandLaboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, 14 Marii Magdaleny Street, 61-861 Poznan, PolandLaboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, 14 Marii Magdaleny Street, 61-861 Poznan, PolandDepartment of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants 27 Libelta Street, 61-707 Poznan, PolandDepartment of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants 27 Libelta Street, 61-707 Poznan, PolandDepartment of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants 27 Libelta Street, 61-707 Poznan, PolandDepartment of Quality Control of Medicinal Products and Dietary Supplements, Institute of Natural Fibres and Medicinal Plants 27 Libelta Street, 61-707 Poznan, PolandDepartment of Gastroenterology and Metabolic Diseases, Medical University of Warsaw, Banacha 1a Street, 02-091 Warsaw,PolandLaboratory of Experimental Pharmacogenetics, Department of Clinical Pharmacy and Biopharmacy, University of Medical Sciences, 14 Marii Magdaleny Street, 61-861 Poznan, PolandThere are a number of compounds that can modify the activity of ABC (ATP-binding cassette) and SLC (solute carrier) transporters in the blood-brain barrier (BBB). The aim of this study was to investigate the effect of natural and synthetic substances on the expression level of genes encoding transporters present in the BBB (mdr1a, mdr1b, mrp1, mrp2, oatp1a4, oatp1a5 and oatp1c1). Our results showed that verapamil caused the greatest reduction in the mRNA level while other synthetic (piracetam, phenobarbital) and natural (codeine, cyclosporine A, quercetin) substances showed a selective inhibitory effect. Further, the extract from the roots of Panax ginseng C. A. Meyer exhibited a decrease of transcription against selected transporters whereas the extract from Ginkgo biloba L. leaves resulted in an increase of the expression level of tested genes, except for mrp2. Extract from the aerial parts of Hypericum perforatum L. was the only one to cause an increased mRNA level for mdr1 and oatp1c1. These findings suggest that herbs can play an important role in overcoming the BBB and multidrug resistance to pharmacotherapy of brain cancer and mental disorders, based on the activity of selected drug-metabolizing enzymes and transporters located in the BBBhttp://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0020/acph-2014-0020.xml?format=INTABC proteinsP-glycoproteinblood-brain barrierexpression levelherbal substances |
| spellingShingle | Mrozikiewicz Przemyslaw M. Bogacz Anna Bartkowiak-Wieczorek Joanna Kujawski Radoslaw Mikolajczak Przemyslaw L. Ozarowski Marcin Czerny Boguslaw Mrozikiewicz-Rakowska Beata Grzeskowiak Edmund Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters Acta Pharmaceutica ABC proteins P-glycoprotein blood-brain barrier expression level herbal substances |
| title | Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters |
| title_full | Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters |
| title_fullStr | Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters |
| title_full_unstemmed | Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters |
| title_short | Screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters |
| title_sort | screening for impact of popular herbs improving mental abilities on the transcriptional level of brain transporters |
| topic | ABC proteins P-glycoprotein blood-brain barrier expression level herbal substances |
| url | http://www.degruyter.com/view/j/acph.2014.64.issue-2/acph-2014-0020/acph-2014-0020.xml?format=INT |
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