Antimicrobial Resistance Patterns of ESBL-Producing <i>Escherichia coli</i> in Dogs from Thailand: Evaluation of Algal Extracts as Novel Antimicrobial Agents
<b>Background/Objectives:</b> Multidrug-resistant (MDR) bacteria, including extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i>, in companion animals pose a growing public health concern due to the close interactions between pets and humans. This study a...
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MDPI AG
2025-04-01
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| Series: | Antibiotics |
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| author | Khomson Satchasataporn Duangdaow Khunbutsri Peechanika Chopjitt Samak Sutjarit Wanida Pan-utai Nattakan Meekhanon |
| author_facet | Khomson Satchasataporn Duangdaow Khunbutsri Peechanika Chopjitt Samak Sutjarit Wanida Pan-utai Nattakan Meekhanon |
| author_sort | Khomson Satchasataporn |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Multidrug-resistant (MDR) bacteria, including extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i>, in companion animals pose a growing public health concern due to the close interactions between pets and humans. This study aimed to investigate antimicrobial resistance patterns and the prevalence of ESBL-producing <i>E. coli</i> isolated from healthy dogs in Thailand, as well as the potential of algal extracts obtained through ethanol extraction and enzymatic hydrolysis as alternative antimicrobial agents against these drug-resistant organisms. <b>Methods:</b> Antimicrobial susceptibility testing was performed on 43 <i>E. coli</i> isolates from healthy dogs. ESBL production was confirmed using standard phenotypic methods, and resistance genes were detected by PCR. The algal extracts were tested for antibacterial activity against MDR isolates. <b>Results:</b> Among the 43 <i>E. coli</i> isolates, 67.44% were classified as MDR, with high resistance rates observed for ampicillin (79.07%), tetracycline (65.12%), and ciprofloxacin (62.79%), highlighting significant antimicrobial resistance concerns. Of the MDR isolates, 31.03% (9/29) were confirmed as ESBL producers. Gene analysis revealed <i>bla</i><sub>TEM</sub> as the most prevalent gene (53.49%), followed by <i>bla</i><sub>CTX-M</sub> (9.30%), while <i>bla</i><sub>SHV</sub> was detected in a single isolate resistant only to ampicillin and was absent in all MDR strains. Ethanol extracts of <i>Haematococcus pluvialis</i> and <i>Caulerpa lentillifera</i> demonstrated inhibitory effects against MDR <i>E. coli</i>. <b>Conclusions:</b> MDR and ESBL-producing <i>E. coli</i> are prevalent in healthy dogs, posing a potential public health risk. Algal extracts from <i>H. pluvialis</i> and <i>C. lentillifera</i> show promise as alternative antimicrobials. Further research is necessary to optimize their efficacy and investigate their in vivo applications, including clinical and environmental settings. |
| format | Article |
| id | doaj-art-6c8f37752baf4f289cde06743e511d42 |
| institution | OA Journals |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-6c8f37752baf4f289cde06743e511d422025-08-20T02:17:25ZengMDPI AGAntibiotics2079-63822025-04-0114437710.3390/antibiotics14040377Antimicrobial Resistance Patterns of ESBL-Producing <i>Escherichia coli</i> in Dogs from Thailand: Evaluation of Algal Extracts as Novel Antimicrobial AgentsKhomson Satchasataporn0Duangdaow Khunbutsri1Peechanika Chopjitt2Samak Sutjarit3Wanida Pan-utai4Nattakan Meekhanon5Department of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, ThailandVeterinary Diagnostic Laboratory, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Public Health, Kasetsart University, Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon 47000, ThailandDepartment of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, ThailandDepartment of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Bangkok 10900, ThailandDepartment of Veterinary Nursing, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand<b>Background/Objectives:</b> Multidrug-resistant (MDR) bacteria, including extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i>, in companion animals pose a growing public health concern due to the close interactions between pets and humans. This study aimed to investigate antimicrobial resistance patterns and the prevalence of ESBL-producing <i>E. coli</i> isolated from healthy dogs in Thailand, as well as the potential of algal extracts obtained through ethanol extraction and enzymatic hydrolysis as alternative antimicrobial agents against these drug-resistant organisms. <b>Methods:</b> Antimicrobial susceptibility testing was performed on 43 <i>E. coli</i> isolates from healthy dogs. ESBL production was confirmed using standard phenotypic methods, and resistance genes were detected by PCR. The algal extracts were tested for antibacterial activity against MDR isolates. <b>Results:</b> Among the 43 <i>E. coli</i> isolates, 67.44% were classified as MDR, with high resistance rates observed for ampicillin (79.07%), tetracycline (65.12%), and ciprofloxacin (62.79%), highlighting significant antimicrobial resistance concerns. Of the MDR isolates, 31.03% (9/29) were confirmed as ESBL producers. Gene analysis revealed <i>bla</i><sub>TEM</sub> as the most prevalent gene (53.49%), followed by <i>bla</i><sub>CTX-M</sub> (9.30%), while <i>bla</i><sub>SHV</sub> was detected in a single isolate resistant only to ampicillin and was absent in all MDR strains. Ethanol extracts of <i>Haematococcus pluvialis</i> and <i>Caulerpa lentillifera</i> demonstrated inhibitory effects against MDR <i>E. coli</i>. <b>Conclusions:</b> MDR and ESBL-producing <i>E. coli</i> are prevalent in healthy dogs, posing a potential public health risk. Algal extracts from <i>H. pluvialis</i> and <i>C. lentillifera</i> show promise as alternative antimicrobials. Further research is necessary to optimize their efficacy and investigate their in vivo applications, including clinical and environmental settings.https://www.mdpi.com/2079-6382/14/4/377algal extractdog<i>Escherichia coli</i>extended-spectrum beta-lactamasemultidrug-resistant |
| spellingShingle | Khomson Satchasataporn Duangdaow Khunbutsri Peechanika Chopjitt Samak Sutjarit Wanida Pan-utai Nattakan Meekhanon Antimicrobial Resistance Patterns of ESBL-Producing <i>Escherichia coli</i> in Dogs from Thailand: Evaluation of Algal Extracts as Novel Antimicrobial Agents Antibiotics algal extract dog <i>Escherichia coli</i> extended-spectrum beta-lactamase multidrug-resistant |
| title | Antimicrobial Resistance Patterns of ESBL-Producing <i>Escherichia coli</i> in Dogs from Thailand: Evaluation of Algal Extracts as Novel Antimicrobial Agents |
| title_full | Antimicrobial Resistance Patterns of ESBL-Producing <i>Escherichia coli</i> in Dogs from Thailand: Evaluation of Algal Extracts as Novel Antimicrobial Agents |
| title_fullStr | Antimicrobial Resistance Patterns of ESBL-Producing <i>Escherichia coli</i> in Dogs from Thailand: Evaluation of Algal Extracts as Novel Antimicrobial Agents |
| title_full_unstemmed | Antimicrobial Resistance Patterns of ESBL-Producing <i>Escherichia coli</i> in Dogs from Thailand: Evaluation of Algal Extracts as Novel Antimicrobial Agents |
| title_short | Antimicrobial Resistance Patterns of ESBL-Producing <i>Escherichia coli</i> in Dogs from Thailand: Evaluation of Algal Extracts as Novel Antimicrobial Agents |
| title_sort | antimicrobial resistance patterns of esbl producing i escherichia coli i in dogs from thailand evaluation of algal extracts as novel antimicrobial agents |
| topic | algal extract dog <i>Escherichia coli</i> extended-spectrum beta-lactamase multidrug-resistant |
| url | https://www.mdpi.com/2079-6382/14/4/377 |
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