Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against <i>Fusobacterium nucleatum</i> Subspecies
<i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>), a key pathogen implicated in periodontal disease, contributes to oral biofilm maturation and is linked to development of systemic diseases like colorectal cancer and liver cirrhosis. Photodynamic therapy (PDT) combined wit...
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MDPI AG
2024-11-01
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| author | Jianglan Li Takayuki Nambu Chao Wang Hiroki Takigawa Hugo Maruyama Chiho Mashimo Toshinori Okinaga |
| author_facet | Jianglan Li Takayuki Nambu Chao Wang Hiroki Takigawa Hugo Maruyama Chiho Mashimo Toshinori Okinaga |
| author_sort | Jianglan Li |
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| description | <i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>), a key pathogen implicated in periodontal disease, contributes to oral biofilm maturation and is linked to development of systemic diseases like colorectal cancer and liver cirrhosis. Photodynamic therapy (PDT) combined with 5-aminolevulinic acid (5-ALA) treatment (ALA-PDT) selectively targets <i>F. nucleatum</i> by inducing porphyrin accumulation. The bactericidal effect of red light-based PDT on <i>F. nucleatum</i> has not been evaluated previously. This study investigates the effect of ALA-PDT using red light-emitting diode (LED) light on <i>F. nucleatum</i> subspecies and their porphyrin accumulation. <i>F. nucleatum</i> subspecies were cultured with varying concentrations of 5-ALA under anaerobic conditions. Porphyrin accumulation was measured via fluorescence spectroscopy, and colony-forming units were measured to determine bacterial viability post-treatment. Additionally, other subspecies responded well to 0.01% 5-ALA, and uroporphyrin I accumulation correlated with bacterial death, revealing optimal bactericidal conditions. These results suggest that optimizing light intensity and 5-ALA concentration can significantly enhance the therapeutic potential of ALA-PDT in oral healthcare. |
| format | Article |
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| issn | 2076-0817 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-6c816aad681247849b432e2c7ccf300f2025-08-20T01:53:54ZengMDPI AGPathogens2076-08172024-11-011311101610.3390/pathogens13111016Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against <i>Fusobacterium nucleatum</i> SubspeciesJianglan Li0Takayuki Nambu1Chao Wang2Hiroki Takigawa3Hugo Maruyama4Chiho Mashimo5Toshinori Okinaga6Graduate School of Dentistry (Bacteriology), Osaka Dental University, Hirakata 573-1121, JapanDepartment of Microbiology, Osaka Dental University, Hirakata 573-1121, JapanCenter of Oral Implantology, Stomatological Hospital, School of Stomatology, Southern Medical University, Guangzhou 510515, ChinaDepartment of Microbiology, Osaka Dental University, Hirakata 573-1121, JapanDepartment of Microbiology, Osaka Dental University, Hirakata 573-1121, JapanDepartment of Microbiology, Osaka Dental University, Hirakata 573-1121, JapanDepartment of Microbiology, Osaka Dental University, Hirakata 573-1121, Japan<i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>), a key pathogen implicated in periodontal disease, contributes to oral biofilm maturation and is linked to development of systemic diseases like colorectal cancer and liver cirrhosis. Photodynamic therapy (PDT) combined with 5-aminolevulinic acid (5-ALA) treatment (ALA-PDT) selectively targets <i>F. nucleatum</i> by inducing porphyrin accumulation. The bactericidal effect of red light-based PDT on <i>F. nucleatum</i> has not been evaluated previously. This study investigates the effect of ALA-PDT using red light-emitting diode (LED) light on <i>F. nucleatum</i> subspecies and their porphyrin accumulation. <i>F. nucleatum</i> subspecies were cultured with varying concentrations of 5-ALA under anaerobic conditions. Porphyrin accumulation was measured via fluorescence spectroscopy, and colony-forming units were measured to determine bacterial viability post-treatment. Additionally, other subspecies responded well to 0.01% 5-ALA, and uroporphyrin I accumulation correlated with bacterial death, revealing optimal bactericidal conditions. These results suggest that optimizing light intensity and 5-ALA concentration can significantly enhance the therapeutic potential of ALA-PDT in oral healthcare.https://www.mdpi.com/2076-0817/13/11/1016<i>Fusobacterium nucleatum</i>photodynamic therapy5-aminolevulinic acidsubspeciesred light-emitting diode |
| spellingShingle | Jianglan Li Takayuki Nambu Chao Wang Hiroki Takigawa Hugo Maruyama Chiho Mashimo Toshinori Okinaga Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against <i>Fusobacterium nucleatum</i> Subspecies Pathogens <i>Fusobacterium nucleatum</i> photodynamic therapy 5-aminolevulinic acid subspecies red light-emitting diode |
| title | Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against <i>Fusobacterium nucleatum</i> Subspecies |
| title_full | Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against <i>Fusobacterium nucleatum</i> Subspecies |
| title_fullStr | Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against <i>Fusobacterium nucleatum</i> Subspecies |
| title_full_unstemmed | Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against <i>Fusobacterium nucleatum</i> Subspecies |
| title_short | Optimizing Red Light-Based Photodynamic Therapy for Effective Bactericidal Action Against <i>Fusobacterium nucleatum</i> Subspecies |
| title_sort | optimizing red light based photodynamic therapy for effective bactericidal action against i fusobacterium nucleatum i subspecies |
| topic | <i>Fusobacterium nucleatum</i> photodynamic therapy 5-aminolevulinic acid subspecies red light-emitting diode |
| url | https://www.mdpi.com/2076-0817/13/11/1016 |
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