Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases
Objective. To determine the expression of cytokines in the iris of patients with neovascular glaucoma (NVG). Methods. Patients with NVG associated with proliferative diabetic retinopathy (PDR, group 1) or central retinal vein occlusion (CRVO, group 2) who had undergone surgical treatment were enroll...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2018/8025951 |
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| _version_ | 1850157907513966592 |
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| author | Heng Miao Xianru Hou De-Kuang Hwang Yong Tao |
| author_facet | Heng Miao Xianru Hou De-Kuang Hwang Yong Tao |
| author_sort | Heng Miao |
| collection | DOAJ |
| description | Objective. To determine the expression of cytokines in the iris of patients with neovascular glaucoma (NVG). Methods. Patients with NVG associated with proliferative diabetic retinopathy (PDR, group 1) or central retinal vein occlusion (CRVO, group 2) who had undergone surgical treatment were enrolled. Patients with primary open-angle glaucoma requiring surgical treatment were included in the control group (group 3). All iris specimens were obtained during trabeculectomy, 7 days after intravitreal injections of ranibizumab. The messenger RNA (mRNA) and protein levels of three target cytokines—vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and pigment epithelium-derived factor (PEDF)—in the iris were analyzed and compared. Results. We included 39 eyes from 39 patients (12, 15, and 12 in groups 1, 2, and 3, resp.). The protein and mRNA levels of PEDF were higher in two NVG groups. The protein levels, but not mRNA level, of bFGF were higher in the two NVG groups. The protein and mRNA levels of VEGF were similar in the three groups. Conclusions. The protein level of bFGF increased in the irises of the NVG patients was not expressed by the iris itself, whereas PEDF may be expressed by the iris tissue in these patients. |
| format | Article |
| id | doaj-art-6c811dc657ae4b19a992a80079c67340 |
| institution | OA Journals |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-6c811dc657ae4b19a992a80079c673402025-08-20T02:24:03ZengWileyJournal of Ophthalmology2090-004X2090-00582018-01-01201810.1155/2018/80259518025951Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal DiseasesHeng Miao0Xianru Hou1De-Kuang Hwang2Yong Tao3Department of Ophthalmology, People’s Hospital, Peking University, Beijing, ChinaDepartment of Ophthalmology, People’s Hospital, Peking University, Beijing, ChinaDepartment of Ophthalmology, Taipei Veterans General Hospital, Taipei, TaiwanDepartment of Ophthalmology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, ChinaObjective. To determine the expression of cytokines in the iris of patients with neovascular glaucoma (NVG). Methods. Patients with NVG associated with proliferative diabetic retinopathy (PDR, group 1) or central retinal vein occlusion (CRVO, group 2) who had undergone surgical treatment were enrolled. Patients with primary open-angle glaucoma requiring surgical treatment were included in the control group (group 3). All iris specimens were obtained during trabeculectomy, 7 days after intravitreal injections of ranibizumab. The messenger RNA (mRNA) and protein levels of three target cytokines—vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and pigment epithelium-derived factor (PEDF)—in the iris were analyzed and compared. Results. We included 39 eyes from 39 patients (12, 15, and 12 in groups 1, 2, and 3, resp.). The protein and mRNA levels of PEDF were higher in two NVG groups. The protein levels, but not mRNA level, of bFGF were higher in the two NVG groups. The protein and mRNA levels of VEGF were similar in the three groups. Conclusions. The protein level of bFGF increased in the irises of the NVG patients was not expressed by the iris itself, whereas PEDF may be expressed by the iris tissue in these patients.http://dx.doi.org/10.1155/2018/8025951 |
| spellingShingle | Heng Miao Xianru Hou De-Kuang Hwang Yong Tao Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases Journal of Ophthalmology |
| title | Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases |
| title_full | Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases |
| title_fullStr | Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases |
| title_full_unstemmed | Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases |
| title_short | Vascular Endothelial Growth Factor, Basic Fibroblast Growth Factor, and Pigment Epithelium-Derived Factor Expression in the Neovascular Iris in Retinal Diseases |
| title_sort | vascular endothelial growth factor basic fibroblast growth factor and pigment epithelium derived factor expression in the neovascular iris in retinal diseases |
| url | http://dx.doi.org/10.1155/2018/8025951 |
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