Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene
Peste des petits ruminants virus (PPRV) has been classified into four lineages based on the nucleocapsid and fusion genes, with lineage IV strains being the most widely distributed. In Africa, recent epidemiological data revealed that PPRV lineage IV is increasingly displacing other lineages in prev...
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2025-07-01
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| Online Access: | https://www.mdpi.com/1999-4915/17/7/976 |
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| author | Jiao Xu Qinghua Wang Jiarong Yu Yingli Wang Huicong Li Lin Li Jingyue Bao Zhiliang Wang |
| author_facet | Jiao Xu Qinghua Wang Jiarong Yu Yingli Wang Huicong Li Lin Li Jingyue Bao Zhiliang Wang |
| author_sort | Jiao Xu |
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| description | Peste des petits ruminants virus (PPRV) has been classified into four lineages based on the nucleocapsid and fusion genes, with lineage IV strains being the most widely distributed. In Africa, recent epidemiological data revealed that PPRV lineage IV is increasingly displacing other lineages in prevalence, suggesting a competitive advantage in viral transmission and adaptability. Moreover, a lineage IV strain was the only confirmed strain in Europe and Asia. In this study, a one-step Taqman quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay for lineage IV PPRV was established by targeting the hemagglutinin (H) gene. The results indicated that this method could detect approximately six copies of PPRV RNA, indicating high sensitivity. No cross-reactions with related viruses or other lineages of PPRV were observed. The results of a repeatability test indicated that the coefficient of variation values were low in both the inter-assay and intra-assay experimental groups. Detection of field samples indicated that all positive samples could be detected successfully using the developed method. This RT-qPCR assay provides a valuable tool to facilitate targeted surveillance and rapid differential diagnosis in regions with active circulation of PPRV lineage IV, enabling timely epidemiological investigations and strain-specific identification. |
| format | Article |
| id | doaj-art-6c7e09600feb44c49f1c62ab95e2d618 |
| institution | DOAJ |
| issn | 1999-4915 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| series | Viruses |
| spelling | doaj-art-6c7e09600feb44c49f1c62ab95e2d6182025-08-20T02:47:09ZengMDPI AGViruses1999-49152025-07-0117797610.3390/v17070976Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin GeneJiao Xu0Qinghua Wang1Jiarong Yu2Yingli Wang3Huicong Li4Lin Li5Jingyue Bao6Zhiliang Wang7China Animal Health and Epidemiology Center, Qingdao, 266000, ChinaChina Animal Health and Epidemiology Center, Qingdao, 266000, ChinaChina Animal Health and Epidemiology Center, Qingdao, 266000, ChinaChina Animal Health and Epidemiology Center, Qingdao, 266000, ChinaCollege of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, ChinaChina Animal Health and Epidemiology Center, Qingdao, 266000, ChinaChina Animal Health and Epidemiology Center, Qingdao, 266000, ChinaChina Animal Health and Epidemiology Center, Qingdao, 266000, ChinaPeste des petits ruminants virus (PPRV) has been classified into four lineages based on the nucleocapsid and fusion genes, with lineage IV strains being the most widely distributed. In Africa, recent epidemiological data revealed that PPRV lineage IV is increasingly displacing other lineages in prevalence, suggesting a competitive advantage in viral transmission and adaptability. Moreover, a lineage IV strain was the only confirmed strain in Europe and Asia. In this study, a one-step Taqman quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay for lineage IV PPRV was established by targeting the hemagglutinin (H) gene. The results indicated that this method could detect approximately six copies of PPRV RNA, indicating high sensitivity. No cross-reactions with related viruses or other lineages of PPRV were observed. The results of a repeatability test indicated that the coefficient of variation values were low in both the inter-assay and intra-assay experimental groups. Detection of field samples indicated that all positive samples could be detected successfully using the developed method. This RT-qPCR assay provides a valuable tool to facilitate targeted surveillance and rapid differential diagnosis in regions with active circulation of PPRV lineage IV, enabling timely epidemiological investigations and strain-specific identification.https://www.mdpi.com/1999-4915/17/7/976peste des petits ruminants virusreverse transcription quantitative polymerase chain reactiondetectiondiagnosis |
| spellingShingle | Jiao Xu Qinghua Wang Jiarong Yu Yingli Wang Huicong Li Lin Li Jingyue Bao Zhiliang Wang Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene Viruses peste des petits ruminants virus reverse transcription quantitative polymerase chain reaction detection diagnosis |
| title | Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene |
| title_full | Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene |
| title_fullStr | Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene |
| title_full_unstemmed | Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene |
| title_short | Detection of Lineage IV Peste Des Petits Ruminants Virus by RT-qPCR Assay via Targeting the Hemagglutinin Gene |
| title_sort | detection of lineage iv peste des petits ruminants virus by rt qpcr assay via targeting the hemagglutinin gene |
| topic | peste des petits ruminants virus reverse transcription quantitative polymerase chain reaction detection diagnosis |
| url | https://www.mdpi.com/1999-4915/17/7/976 |
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