In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L.
The objective of this research was to identify morphogenesis responses of in vivo and in vitro-derived explant of Physalis angulata L. Explants were cultured on MS medium containing 3% sucrose solidified with 1.3% agar supplemented with combination of 6-benzylaminopurine (BAP), indole acetic acids (...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Brawijaya
2017-12-01
|
| Series: | Journal of Experimental Life Science |
| Subjects: | |
| Online Access: | https://jels.ub.ac.id/index.php/jels/article/view/239 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849327823025602560 |
|---|---|
| author | Retno Mastuti Aminatun Munawarti |
| author_facet | Retno Mastuti Aminatun Munawarti |
| author_sort | Retno Mastuti |
| collection | DOAJ |
| description | The objective of this research was to identify morphogenesis responses of in vivo and in vitro-derived explant of Physalis angulata L. Explants were cultured on MS medium containing 3% sucrose solidified with 1.3% agar supplemented with combination of 6-benzylaminopurine (BAP), indole acetic acids (IAA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). All explant types have morphogenesis ability. Capability for shoot regeneration was observed in high number of shoot tip (96.1%) and nodes explants (75.2%). Callus formation was dominantly produced by in vitro leaf explants. In vivo leaf had low responses for shoot/root regeneration and callus formation. These results provided an alternative choice of the explant suitable for either plant regeneration or callus formation. |
| format | Article |
| id | doaj-art-6c7bf46eb0414c0cab5bd3973bfa55f3 |
| institution | Kabale University |
| issn | 2087-2852 2338-1655 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | University of Brawijaya |
| record_format | Article |
| series | Journal of Experimental Life Science |
| spelling | doaj-art-6c7bf46eb0414c0cab5bd3973bfa55f32025-08-20T03:47:45ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552017-12-0172102205https://doi.org/10.21776/ub.jels.2017.007.02.07In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L.Retno Mastuti0Aminatun Munawarti1Universitas BrawijayaUniversitas BrawijayaThe objective of this research was to identify morphogenesis responses of in vivo and in vitro-derived explant of Physalis angulata L. Explants were cultured on MS medium containing 3% sucrose solidified with 1.3% agar supplemented with combination of 6-benzylaminopurine (BAP), indole acetic acids (IAA), naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). All explant types have morphogenesis ability. Capability for shoot regeneration was observed in high number of shoot tip (96.1%) and nodes explants (75.2%). Callus formation was dominantly produced by in vitro leaf explants. In vivo leaf had low responses for shoot/root regeneration and callus formation. These results provided an alternative choice of the explant suitable for either plant regeneration or callus formation.https://jels.ub.ac.id/index.php/jels/article/view/239calluscotyledonary nodesexplant typesshoot regeneration |
| spellingShingle | Retno Mastuti Aminatun Munawarti In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L. Journal of Experimental Life Science callus cotyledonary nodes explant types shoot regeneration |
| title | In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L. |
| title_full | In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L. |
| title_fullStr | In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L. |
| title_full_unstemmed | In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L. |
| title_short | In Vitro Morphogenesis Responses of Various Explant in Physalis angulata L. |
| title_sort | in vitro morphogenesis responses of various explant in physalis angulata l |
| topic | callus cotyledonary nodes explant types shoot regeneration |
| url | https://jels.ub.ac.id/index.php/jels/article/view/239 |
| work_keys_str_mv | AT retnomastuti invitromorphogenesisresponsesofvariousexplantinphysalisangulatal AT aminatunmunawarti invitromorphogenesisresponsesofvariousexplantinphysalisangulatal |