Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, Iran
Background: In Cystic Fibrosis (CF) patients, increased mucus secretion and accumulation due to genetic defect leads to decreased oxygen levels and colonization of anaerobic bacteria. Aim and Objectives: In addition to investigating the difference in the prevalence of anaerobic bacteria in the sputu...
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| Language: | English |
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Krishna Vishwa Vidyapeeth (Deemed to be University), Karad
2024-07-01
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| Series: | Journal of Krishna Institute of Medical Sciences University |
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| Online Access: | https://www.jkimsu.com/jkimsu-vol13no3/JKIMSU,%20Vol.%2013,%20No.%203,%20July-September%202024%20Page%2046-59.pdf |
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| author | Homa Hamayeli Ahya Abdi-Ali Bahareh Attaran Morvarid Shafiei Mohammadreza Modaresi |
| author_facet | Homa Hamayeli Ahya Abdi-Ali Bahareh Attaran Morvarid Shafiei Mohammadreza Modaresi |
| author_sort | Homa Hamayeli |
| collection | DOAJ |
| description | Background: In Cystic Fibrosis (CF) patients, increased mucus secretion and accumulation due to genetic defect leads to decreased oxygen levels and colonization of anaerobic bacteria. Aim and Objectives: In addition to investigating the difference in the prevalence of anaerobic bacteria in the sputum samples of CF patients and healthy populations, this study evaluated the relationship of demographic factors with anaerobic bacteria and CF disease. Material and Methods: Anaerobic bacteria were investigated in sputum samples of CF patients (50 people) and healthy individuals (18 people) in Tehran, Iran by molecular method in 2021-2022. The prevalence of common anaerobic bacteria in CF patients was investigated by Polymerase Chain Reaction (PCR). Results: The relationship between Bifidobacterium and Prevotella in both of healthy and CF groups, as well as the disease and Bifidobacterium (p = 0.0437) and disease
and Prevotella (p = 0.0514), were significant. The results of Spearman's correlation also showed an inverse relationship between age and gender (men) with disease and a direct relationship between antibiotic use and disease.
Conclusions: The abundance of potentially beneficial species of Bifidobacterium and Prevotella was lower in CF than in healthy people. The role of anaerobic bacteria in the lungs of CF patients is different according to the bacterial genus
and species. |
| format | Article |
| id | doaj-art-6c78fd111392476c8c165898291122f5 |
| institution | Kabale University |
| issn | 2231-4261 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Krishna Vishwa Vidyapeeth (Deemed to be University), Karad |
| record_format | Article |
| series | Journal of Krishna Institute of Medical Sciences University |
| spelling | doaj-art-6c78fd111392476c8c165898291122f52025-02-02T02:44:17ZengKrishna Vishwa Vidyapeeth (Deemed to be University), KaradJournal of Krishna Institute of Medical Sciences University2231-42612024-07-011334659Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, IranHoma Hamayeli 0 Ahya Abdi-Ali1Bahareh Attaran2Morvarid Shafiei3Mohammadreza Modaresi4Department of Microbiology, Faculty of Biological Sciences, Alzahra University- 1993893973 (Tehran) IranDepartment of Microbiology, Faculty of Biological Sciences, Alzahra University- 1993893973 (Tehran) IranDepartment of Microbiology, Faculty of Biological Sciences, Alzahra University- 1993893973 (Tehran) IranDepartment of Bacteriology, Pasteur Institute of Iran-1316943551 (Tehran) IranGrowth and Development Research Center, Tehran University of Medical Sciences-1416753955 (Tehran) Iran, Pediatric Pulmonary Disease and Sleep Medicine Research Center, Pediatric Centre of Excellence, Children's Medical Center, Tehran University of Medical Sciences-1416753955 (Tehran) IranBackground: In Cystic Fibrosis (CF) patients, increased mucus secretion and accumulation due to genetic defect leads to decreased oxygen levels and colonization of anaerobic bacteria. Aim and Objectives: In addition to investigating the difference in the prevalence of anaerobic bacteria in the sputum samples of CF patients and healthy populations, this study evaluated the relationship of demographic factors with anaerobic bacteria and CF disease. Material and Methods: Anaerobic bacteria were investigated in sputum samples of CF patients (50 people) and healthy individuals (18 people) in Tehran, Iran by molecular method in 2021-2022. The prevalence of common anaerobic bacteria in CF patients was investigated by Polymerase Chain Reaction (PCR). Results: The relationship between Bifidobacterium and Prevotella in both of healthy and CF groups, as well as the disease and Bifidobacterium (p = 0.0437) and disease and Prevotella (p = 0.0514), were significant. The results of Spearman's correlation also showed an inverse relationship between age and gender (men) with disease and a direct relationship between antibiotic use and disease. Conclusions: The abundance of potentially beneficial species of Bifidobacterium and Prevotella was lower in CF than in healthy people. The role of anaerobic bacteria in the lungs of CF patients is different according to the bacterial genus and species.https://www.jkimsu.com/jkimsu-vol13no3/JKIMSU,%20Vol.%2013,%20No.%203,%20July-September%202024%20Page%2046-59.pdfanaerobic bacteriabifidobacteriumcystic fibrosislung infectionprevotella |
| spellingShingle | Homa Hamayeli Ahya Abdi-Ali Bahareh Attaran Morvarid Shafiei Mohammadreza Modaresi Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, Iran Journal of Krishna Institute of Medical Sciences University anaerobic bacteria bifidobacterium cystic fibrosis lung infection prevotella |
| title | Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, Iran |
| title_full | Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, Iran |
| title_fullStr | Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, Iran |
| title_full_unstemmed | Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, Iran |
| title_short | Investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in Tehran, Iran |
| title_sort | investigation of some anaerobic bacteria in the sputum of cystic fibrosis patients and healthy people in tehran iran |
| topic | anaerobic bacteria bifidobacterium cystic fibrosis lung infection prevotella |
| url | https://www.jkimsu.com/jkimsu-vol13no3/JKIMSU,%20Vol.%2013,%20No.%203,%20July-September%202024%20Page%2046-59.pdf |
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