Construction of a brassinosteroids supersensitive chimeric receptor and rice transformation
A chimeric receptor kinase gene SNRG1 was constructed by combining the Arabidopsis SUD1 (brassinosteroid (BR) supersensitive receptor kinase) LRR-JM domain with the rice XA21 (disease resistance receptor kinase) kinase domain with point mutagenisis in vitro. The SUD1 LRR mutant SNRGmL and the XA21 k...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2004-09-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/1008-9209.2004.05.0482 |
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| Summary: | A chimeric receptor kinase gene SNRG1 was constructed by combining the Arabidopsis SUD1 (brassinosteroid (BR) supersensitive receptor kinase) LRR-JM domain with the rice XA21 (disease resistance receptor kinase) kinase domain with point mutagenisis in vitro. The SUD1 LRR mutant SNRGmL and the XA21 kinase mutant SNRGmK were also constructed as controls respectively. Using particle bombardment, SNRG1, SNRGmL and SNRGmK were successfully transferred into rice embryogenic calli of japonica variety Taipei 309. Transgenic rice plants and cell lines were confirmed by PCR, Northern blot and transformant phenotype. Preliminary result showed that rice defense pathways could have been activated by trace amount of endogenous BR in the SNRG1 transgenic plants. These results provided the foundation for further investigation of XA21 kinase activation upon BR ligand binding. |
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| ISSN: | 1008-9209 2097-5155 |