Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique

Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique

Summary: Endothelial cells (ECs) play a major role in vascular homeostasis and pathology. They show a pronounced heterogeneity between different organs as well as within the same organ. Here, we present a protocol for the rapid site-specific isolation of murine aortic ECs. We describe steps for vess...

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Bibliographic Details
Main Authors: Alexander Brückner, Adrian Brandtner, Sarah Rieck, Bernd K. Fleischmann, Daniela Wenzel
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Cell isolation
Cell separation/fractionation
Health Sciences
Microscopy
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000267
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