Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique
Summary: Endothelial cells (ECs) play a major role in vascular homeostasis and pathology. They show a pronounced heterogeneity between different organs as well as within the same organ. Here, we present a protocol for the rapid site-specific isolation of murine aortic ECs. We describe steps for vess...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000267 |
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| author | Alexander Brückner Adrian Brandtner Sarah Rieck Bernd K. Fleischmann Daniela Wenzel |
| author_facet | Alexander Brückner Adrian Brandtner Sarah Rieck Bernd K. Fleischmann Daniela Wenzel |
| author_sort | Alexander Brückner |
| collection | DOAJ |
| description | Summary: Endothelial cells (ECs) play a major role in vascular homeostasis and pathology. They show a pronounced heterogeneity between different organs as well as within the same organ. Here, we present a protocol for the rapid site-specific isolation of murine aortic ECs. We describe steps for vessel preparation and the isolation of EC layers by freezing on glass coverslips using a modified Häutchen technique. This protocol can be adapted to investigate surface cells from other tissues as well.For complete details on the use and execution of this protocol, please refer to Brückner et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-6c70a642bb4d496999ee8d879be0b3ca |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-6c70a642bb4d496999ee8d879be0b3ca2025-02-08T05:01:16ZengElsevierSTAR Protocols2666-16672025-03-0161103620Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen techniqueAlexander Brückner0Adrian Brandtner1Sarah Rieck2Bernd K. Fleischmann3Daniela Wenzel4Institute of Physiology I, Medical Faculty, University of Bonn, Bonn, GermanyInstitute of Physiology I, Medical Faculty, University of Bonn, Bonn, GermanyInstitute of Physiology I, Medical Faculty, University of Bonn, Bonn, GermanyInstitute of Physiology I, Medical Faculty, University of Bonn, Bonn, GermanyInstitute of Physiology I, Medical Faculty, University of Bonn, Bonn, Germany; Department of Systems Physiology, Medical Faculty, Ruhr University of Bochum, Bochum, Germany; Corresponding authorSummary: Endothelial cells (ECs) play a major role in vascular homeostasis and pathology. They show a pronounced heterogeneity between different organs as well as within the same organ. Here, we present a protocol for the rapid site-specific isolation of murine aortic ECs. We describe steps for vessel preparation and the isolation of EC layers by freezing on glass coverslips using a modified Häutchen technique. This protocol can be adapted to investigate surface cells from other tissues as well.For complete details on the use and execution of this protocol, please refer to Brückner et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000267Cell isolationCell separation/fractionationHealth SciencesMicroscopyGene Expression |
| spellingShingle | Alexander Brückner Adrian Brandtner Sarah Rieck Bernd K. Fleischmann Daniela Wenzel Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique STAR Protocols Cell isolation Cell separation/fractionation Health Sciences Microscopy Gene Expression |
| title | Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique |
| title_full | Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique |
| title_fullStr | Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique |
| title_full_unstemmed | Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique |
| title_short | Protocol for the site-specific isolation of mouse endothelial cells using the modified Häutchen technique |
| title_sort | protocol for the site specific isolation of mouse endothelial cells using the modified hautchen technique |
| topic | Cell isolation Cell separation/fractionation Health Sciences Microscopy Gene Expression |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000267 |
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