Design of a GFP reporter for splicing analysis in mammalian cells

Design of a GFP reporter for splicing analysis in mammalian cells

Eukaryotic genes are formed by exons and introns. Pre-mRNA splicing promotes exon ligation and intron removal and is performed by a specialized macromolecular machinery named spliceosome, composed of five small ribonucleoprotein particles (snRNPs) and more than one hundred proteins. The activity of...

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Bibliographic Details
Main Authors: Arthur T. Menezes, Helder Y. Nagasse, Hilan R. M. Lopes, Patricia P. Coltri
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Biotechnology Reports
Subjects:
Pre-mRNA splicing
GFP
Reporter system
Online Access:http://www.sciencedirect.com/science/article/pii/S2215017X25000141
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