Effect of Cell Growth and Proliferation Factors (EGF/PDGF Signaling Pathway) on the Etiopathogenesis of Intrauterine Growth Restriction
Objective: It is likely that the subgroups of the epidermal growth factor/platelet-derived growth factor (EGF/PDGF) signaling pathway play a role in the etiopathogenesis of intrauterine growth restriction (IUGR). This study was planned to understand the molecular genetic level of apoptosis in IUGR....
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2024-01-01
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| Series: | Gazi Medical Journal |
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| Online Access: | https://gazimedj.com/articles/effect-of-cell-growth-and-proliferation-factors-egfpdgf-signaling-pathway-on-the-etiopathogenesis-of-intrauterine-growth-restriction/doi/gmj.galenos.2023.3635 |
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| author | Serhan Can İşcan Erhan Demirdağ Melek Yaman Emin Ümit Bağrıacık Merih Bayram |
| author_facet | Serhan Can İşcan Erhan Demirdağ Melek Yaman Emin Ümit Bağrıacık Merih Bayram |
| author_sort | Serhan Can İşcan |
| collection | DOAJ |
| description | Objective: It is likely that the subgroups of the epidermal growth factor/platelet-derived growth factor (EGF/PDGF) signaling pathway play a role in the etiopathogenesis of intrauterine growth restriction (IUGR). This study was planned to understand the molecular genetic level of apoptosis in IUGR.
Method: The EGF/PDGF signaling pathway gene profile (40 genes) was investigated using a real-time reverse transcriptase-polymerase chain reaction. The gene expressions of the IUGR group were compared both individually and as a group. Individual gene differences were also evaluated. The genes related to cell survival and growth, which include the gene groups of apoptosis, cell cycle, cell differentiation, cell growth, cell motility, and cell proliferation, were investigated using microarray technology.
Results: Parity, gestational age at delivery, and APGAR scores at the first and fifth minutes were not significantly different between the IUGR and control groups. However, the women in the IUGR group were younger and slimmer. PRKCA was the only gene with a significant difference in expression between the IUGR and control groups. Nevertheless, individual differences were detected in gene expression associated with cell cycle, differentiation, growth, motility, proliferation, and apoptosis.
Conclusion: Variations in gene expression during pregnancy cause changes in placental and fetal development by affecting apoptosis and cellular events at different levels. The genetic causes of IUGR can be revealed by investigating these metabolic pathways. This study differs from previous IUGR studies, which focused on one or a few genes, because all the gene groups in the EGF/PDGF pathway that may be associated with IUGR were investigated. |
| format | Article |
| id | doaj-art-6c6ae99cd84447b497dbaf01aa8412aa |
| institution | Kabale University |
| issn | 2147-2092 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Gazi Medical Journal |
| spelling | doaj-art-6c6ae99cd84447b497dbaf01aa8412aa2025-01-17T11:07:24ZengGalenos Publishing HouseGazi Medical Journal2147-20922024-01-01351192310.12996/gmj.galenos.2023.3635Effect of Cell Growth and Proliferation Factors (EGF/PDGF Signaling Pathway) on the Etiopathogenesis of Intrauterine Growth RestrictionSerhan Can İşcan0https://orcid.org/0000-0002-3824-5818Erhan Demirdağ1https://orcid.org/0000-0003-4599-3854Melek Yaman2https://orcid.org/0000-0002-6281-0880Emin Ümit Bağrıacık3https://orcid.org/0000-0002-8066-5816Merih Bayram4https://orcid.org/0000-0003-1299-2433Isparta City Hospital, Clinic of Gynecologic Oncology, Isparta, TürkiyeGazi University Faculty of Medicine, Department of Obstetrics and Gynecology, Ankara, TürkiyeGazi University Faculty of Medicine, Department of Immunology, Ankara, TürkiyeGazi University Faculty of Medicine, Department of Immunology, Ankara, TürkiyeGazi University Faculty of Medicine, Department of Obstetrics and Gynecology, Ankara, TürkiyeObjective: It is likely that the subgroups of the epidermal growth factor/platelet-derived growth factor (EGF/PDGF) signaling pathway play a role in the etiopathogenesis of intrauterine growth restriction (IUGR). This study was planned to understand the molecular genetic level of apoptosis in IUGR. Method: The EGF/PDGF signaling pathway gene profile (40 genes) was investigated using a real-time reverse transcriptase-polymerase chain reaction. The gene expressions of the IUGR group were compared both individually and as a group. Individual gene differences were also evaluated. The genes related to cell survival and growth, which include the gene groups of apoptosis, cell cycle, cell differentiation, cell growth, cell motility, and cell proliferation, were investigated using microarray technology. Results: Parity, gestational age at delivery, and APGAR scores at the first and fifth minutes were not significantly different between the IUGR and control groups. However, the women in the IUGR group were younger and slimmer. PRKCA was the only gene with a significant difference in expression between the IUGR and control groups. Nevertheless, individual differences were detected in gene expression associated with cell cycle, differentiation, growth, motility, proliferation, and apoptosis. Conclusion: Variations in gene expression during pregnancy cause changes in placental and fetal development by affecting apoptosis and cellular events at different levels. The genetic causes of IUGR can be revealed by investigating these metabolic pathways. This study differs from previous IUGR studies, which focused on one or a few genes, because all the gene groups in the EGF/PDGF pathway that may be associated with IUGR were investigated.https://gazimedj.com/articles/effect-of-cell-growth-and-proliferation-factors-egfpdgf-signaling-pathway-on-the-etiopathogenesis-of-intrauterine-growth-restriction/doi/gmj.galenos.2023.3635intrauterine growth restrictioncell survival and growthapoptosisegf/pdgfpcr arrayrt-pcr |
| spellingShingle | Serhan Can İşcan Erhan Demirdağ Melek Yaman Emin Ümit Bağrıacık Merih Bayram Effect of Cell Growth and Proliferation Factors (EGF/PDGF Signaling Pathway) on the Etiopathogenesis of Intrauterine Growth Restriction Gazi Medical Journal intrauterine growth restriction cell survival and growth apoptosis egf/pdgf pcr array rt-pcr |
| title | Effect of Cell Growth and Proliferation Factors (EGF/PDGF Signaling Pathway) on the Etiopathogenesis of Intrauterine Growth Restriction |
| title_full | Effect of Cell Growth and Proliferation Factors (EGF/PDGF Signaling Pathway) on the Etiopathogenesis of Intrauterine Growth Restriction |
| title_fullStr | Effect of Cell Growth and Proliferation Factors (EGF/PDGF Signaling Pathway) on the Etiopathogenesis of Intrauterine Growth Restriction |
| title_full_unstemmed | Effect of Cell Growth and Proliferation Factors (EGF/PDGF Signaling Pathway) on the Etiopathogenesis of Intrauterine Growth Restriction |
| title_short | Effect of Cell Growth and Proliferation Factors (EGF/PDGF Signaling Pathway) on the Etiopathogenesis of Intrauterine Growth Restriction |
| title_sort | effect of cell growth and proliferation factors egf pdgf signaling pathway on the etiopathogenesis of intrauterine growth restriction |
| topic | intrauterine growth restriction cell survival and growth apoptosis egf/pdgf pcr array rt-pcr |
| url | https://gazimedj.com/articles/effect-of-cell-growth-and-proliferation-factors-egfpdgf-signaling-pathway-on-the-etiopathogenesis-of-intrauterine-growth-restriction/doi/gmj.galenos.2023.3635 |
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