DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBIT
Background: Hydroxyapatite is a bone graft that has osteoconductive properties for bone defects treatment. Platelet-rich plasma (PRP) has a capability of providing many bioactive molecules in physiological proportions. Hydroxyapatite given freeze-dried PRP is expected to create a graft that can stre...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2020-10-01
|
| Series: | Journal Orthopaedi and Traumatology Surabaya |
| Subjects: | |
| Online Access: | https://e-journal.unair.ac.id/JOINTS/article/view/20791 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856501724282880 |
|---|---|
| author | Mouli Edward Henry Dominica Ferdiansyah Mahyudin Fedik Abdul Rantam |
| author_facet | Mouli Edward Henry Dominica Ferdiansyah Mahyudin Fedik Abdul Rantam |
| author_sort | Mouli Edward |
| collection | DOAJ |
| description | Background: Hydroxyapatite is a bone graft that has osteoconductive properties for bone defects treatment. Platelet-rich plasma (PRP) has a capability of providing many bioactive molecules in physiological proportions. Hydroxyapatite given freeze-dried PRP is expected to create a graft that can strengthen the matrix while promoting osteoinduction. This study compares the effects of regeneration on the bone between bovine hydroxyapatite (BHA) and bovine hydroxyapatite with freeze-dried platelet-rich plasma (FD-PRP) as a bone graft in bone defect of the femoral white rabbit.
Methods: The 12 equal New Zealand white rabbits aged 6-9 months are divided into two groups. Bone defects were made in the lower femoral meta-diaphysis with a diameter of 2.5 mm. The defects were filled with BHA with FD-PRP allograft in the treatment group and BHA in the control group. Both groups will be sacrificed in the third and sixth weeks, then evaluated histologically for microvascular structure, osteoblasts, woven bone, type-I collagen, osteocalcin, alkaline phosphatase, and immunoglobulin G.
Results: During the evaluation in week 3 and 6, microvascular structure, osteoblast, and type-I collagen decreased in both groups with insignificant differences (p>0.05). Woven bone, osteocalcin, and immunoglobulin G increased in the treatment group but was not significant (p>0.05). Alkaline phosphatase increased higher in the treatment group, with a considerable difference in the sixth week (p=0.008).
Conclusion: The elevation in the production of woven bone, osteocalcin, and alkaline phosphatase at the third and sixth-week evaluations highlight the possibility that administering BHA given FD-PRP may have contributed to the healing of bone defects. |
| format | Article |
| id | doaj-art-6c68ba7fc3e4428fbd8bc83286c6d641 |
| institution | Kabale University |
| issn | 2722-712X 2460-8742 |
| language | English |
| publishDate | 2020-10-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal Orthopaedi and Traumatology Surabaya |
| spelling | doaj-art-6c68ba7fc3e4428fbd8bc83286c6d6412025-02-12T07:28:43ZengUniversitas AirlanggaJournal Orthopaedi and Traumatology Surabaya2722-712X2460-87422020-10-0192345410.20473/joints.v9i2.2020.34-5417032DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBITMouli Edward0https://orcid.org/0000-0002-1667-9770Henry Dominica1https://orcid.org/0000-0001-8979-3446Ferdiansyah Mahyudin2https://orcid.org/0000-0001-8757-9251Fedik Abdul Rantam3https://orcid.org/0000-0002-1047-2410Department of Orthopedic and Traumatology, Faculty of Medicine, Universitas Airlangga/ Dr. Soetomo General Hospital, SurabayaDepartment of Orthopedic and Traumatology, Faculty of Medicine, Universitas Airlangga/ Dr. Soetomo General Hospital, Surabaya(SCOPUS ID: 57148390100), Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Airlangga/ Dr. Soetomo General Hospital, SurabayaDepartment of of Virology and immunology, and stem cell, molecular biology, Airlangga UniversityBackground: Hydroxyapatite is a bone graft that has osteoconductive properties for bone defects treatment. Platelet-rich plasma (PRP) has a capability of providing many bioactive molecules in physiological proportions. Hydroxyapatite given freeze-dried PRP is expected to create a graft that can strengthen the matrix while promoting osteoinduction. This study compares the effects of regeneration on the bone between bovine hydroxyapatite (BHA) and bovine hydroxyapatite with freeze-dried platelet-rich plasma (FD-PRP) as a bone graft in bone defect of the femoral white rabbit. Methods: The 12 equal New Zealand white rabbits aged 6-9 months are divided into two groups. Bone defects were made in the lower femoral meta-diaphysis with a diameter of 2.5 mm. The defects were filled with BHA with FD-PRP allograft in the treatment group and BHA in the control group. Both groups will be sacrificed in the third and sixth weeks, then evaluated histologically for microvascular structure, osteoblasts, woven bone, type-I collagen, osteocalcin, alkaline phosphatase, and immunoglobulin G. Results: During the evaluation in week 3 and 6, microvascular structure, osteoblast, and type-I collagen decreased in both groups with insignificant differences (p>0.05). Woven bone, osteocalcin, and immunoglobulin G increased in the treatment group but was not significant (p>0.05). Alkaline phosphatase increased higher in the treatment group, with a considerable difference in the sixth week (p=0.008). Conclusion: The elevation in the production of woven bone, osteocalcin, and alkaline phosphatase at the third and sixth-week evaluations highlight the possibility that administering BHA given FD-PRP may have contributed to the healing of bone defects.https://e-journal.unair.ac.id/JOINTS/article/view/20791alkaline phosphatasebone graftbovine hydroxyapatitefreeze-dried platelet-rich plasmahuman and medicine |
| spellingShingle | Mouli Edward Henry Dominica Ferdiansyah Mahyudin Fedik Abdul Rantam DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBIT Journal Orthopaedi and Traumatology Surabaya alkaline phosphatase bone graft bovine hydroxyapatite freeze-dried platelet-rich plasma human and medicine |
| title | DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBIT |
| title_full | DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBIT |
| title_fullStr | DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBIT |
| title_full_unstemmed | DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBIT |
| title_short | DIFFERENCES OF BONE REGENERATION USING BOVINE HYDROXYAPATITE AND BOVINE HYDROXYAPATITE WITH FREEZE-DRIED PLATELET RICH PLASMA ALLOGRAFT IN BONE DEFECT OF FEMORAL WHITE RABBIT |
| title_sort | differences of bone regeneration using bovine hydroxyapatite and bovine hydroxyapatite with freeze dried platelet rich plasma allograft in bone defect of femoral white rabbit |
| topic | alkaline phosphatase bone graft bovine hydroxyapatite freeze-dried platelet-rich plasma human and medicine |
| url | https://e-journal.unair.ac.id/JOINTS/article/view/20791 |
| work_keys_str_mv | AT mouliedward differencesofboneregenerationusingbovinehydroxyapatiteandbovinehydroxyapatitewithfreezedriedplateletrichplasmaallograftinbonedefectoffemoralwhiterabbit AT henrydominica differencesofboneregenerationusingbovinehydroxyapatiteandbovinehydroxyapatitewithfreezedriedplateletrichplasmaallograftinbonedefectoffemoralwhiterabbit AT ferdiansyahmahyudin differencesofboneregenerationusingbovinehydroxyapatiteandbovinehydroxyapatitewithfreezedriedplateletrichplasmaallograftinbonedefectoffemoralwhiterabbit AT fedikabdulrantam differencesofboneregenerationusingbovinehydroxyapatiteandbovinehydroxyapatitewithfreezedriedplateletrichplasmaallograftinbonedefectoffemoralwhiterabbit |