The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular Mechanism

Rapeseed is the largest self-produced oil crop in China which plays an important role in ensuring the safety of edible oil. However, its current per unit yield is far below Canada and Europe. In this study, selenium fertilizer and other micro fertilizers were sprayed on Xiangzayou 787 at the seedlin...

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Main Authors: Qi Zhang, Jiayuan Peng, Yuqi Liu, Chunfeng Xie, Zhenqian Zhang
Format: Article
Language:English
Published: MDPI AG 2024-09-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/9/2032
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author Qi Zhang
Jiayuan Peng
Yuqi Liu
Chunfeng Xie
Zhenqian Zhang
author_facet Qi Zhang
Jiayuan Peng
Yuqi Liu
Chunfeng Xie
Zhenqian Zhang
author_sort Qi Zhang
collection DOAJ
description Rapeseed is the largest self-produced oil crop in China which plays an important role in ensuring the safety of edible oil. However, its current per unit yield is far below Canada and Europe. In this study, selenium fertilizer and other micro fertilizers were sprayed on Xiangzayou 787 at the seedling stage. The results showed that the yield per plant increased 24.3% with sprayed selenium compared to the control (CK). Compared with the CK, the chlorophyll content in leaves at the flowering stage was significantly increased by 20.8%, and the soluble sugar content in the silique wall and seeds at the maturity stage was significantly increased and increased by 62.1% during the budding stage. The functional leaves of Xiangzayou 787 with the sprayed selenium fertilizer and the CK were used as raw materials for absolute quantitative transcriptome sequencing analysis. Accompanied with bioinformatics analysis, six differential genes which affect growth were discovered. The expression level of the protein phosphatase 2C gene in the silique wall was significantly higher than that of the CK. The <i>PP2C78</i> gene was significantly positively correlated with the chlorophyll and soluble sugar content in leaves and the correlation coefficients were 0.539 and 0.547. According to gene expression levels, yield, and physiological indicators, <i>PP2C78</i> may be a key functional gene affecting rapeseed yield. In this study, selenium fertilizer was found to be an excellent foliar fertilizer for rapeseed; the <i>PP2C78</i> gene may be helpful for analyzing the yield increasing mechanism and used as a reference for screening new foliar fertilizers.
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spelling doaj-art-6c38cc40bcbb4b389541ef0307ffa1bf2025-08-20T01:56:09ZengMDPI AGAgronomy2073-43952024-09-01149203210.3390/agronomy14092032The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular MechanismQi Zhang0Jiayuan Peng1Yuqi Liu2Chunfeng Xie3Zhenqian Zhang4College of Agriculture, Agricultural University of Hunan, 1 Agricultural Road, Changsha 410128, ChinaCollege of Agriculture, Agricultural University of Hunan, 1 Agricultural Road, Changsha 410128, ChinaCollege of Agriculture, Agricultural University of Hunan, 1 Agricultural Road, Changsha 410128, ChinaZhangjiajie Agriculture and Rural Bureau, Zhangjiajie 427000, ChinaCollege of Agriculture, Agricultural University of Hunan, 1 Agricultural Road, Changsha 410128, ChinaRapeseed is the largest self-produced oil crop in China which plays an important role in ensuring the safety of edible oil. However, its current per unit yield is far below Canada and Europe. In this study, selenium fertilizer and other micro fertilizers were sprayed on Xiangzayou 787 at the seedling stage. The results showed that the yield per plant increased 24.3% with sprayed selenium compared to the control (CK). Compared with the CK, the chlorophyll content in leaves at the flowering stage was significantly increased by 20.8%, and the soluble sugar content in the silique wall and seeds at the maturity stage was significantly increased and increased by 62.1% during the budding stage. The functional leaves of Xiangzayou 787 with the sprayed selenium fertilizer and the CK were used as raw materials for absolute quantitative transcriptome sequencing analysis. Accompanied with bioinformatics analysis, six differential genes which affect growth were discovered. The expression level of the protein phosphatase 2C gene in the silique wall was significantly higher than that of the CK. The <i>PP2C78</i> gene was significantly positively correlated with the chlorophyll and soluble sugar content in leaves and the correlation coefficients were 0.539 and 0.547. According to gene expression levels, yield, and physiological indicators, <i>PP2C78</i> may be a key functional gene affecting rapeseed yield. In this study, selenium fertilizer was found to be an excellent foliar fertilizer for rapeseed; the <i>PP2C78</i> gene may be helpful for analyzing the yield increasing mechanism and used as a reference for screening new foliar fertilizers.https://www.mdpi.com/2073-4395/14/9/2032selenium fertilizerXiangzayou 787chlorophyllsoluble sugarprotein phosphatase 2C gene
spellingShingle Qi Zhang
Jiayuan Peng
Yuqi Liu
Chunfeng Xie
Zhenqian Zhang
The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular Mechanism
Agronomy
selenium fertilizer
Xiangzayou 787
chlorophyll
soluble sugar
protein phosphatase 2C gene
title The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular Mechanism
title_full The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular Mechanism
title_fullStr The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular Mechanism
title_full_unstemmed The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular Mechanism
title_short The Study of Selenium Fertilizer on the Growth of Xiangzayou 787 and Related Molecular Mechanism
title_sort study of selenium fertilizer on the growth of xiangzayou 787 and related molecular mechanism
topic selenium fertilizer
Xiangzayou 787
chlorophyll
soluble sugar
protein phosphatase 2C gene
url https://www.mdpi.com/2073-4395/14/9/2032
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