Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii
Introduction: The spread of carbapenem-resistant Acinetobacter baumannii (CRAB) is difficult to control especially in the hospitals due to the successful mobilization and evolution of the genetic elements harboring the resistant determinants. The study was conducted to examine the distribution of a...
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The Journal of Infection in Developing Countries
2019-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/11410 |
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| author | Mohsin Khurshid Muhammad Hidayat Rasool Muhammad Hussnain Siddique Farrukh Azeem Muhammad Naeem Muhammad Sohail Muhammad Sarfraz Muhammad Saqalein Zeeshan Taj Muhammad Atif Nisar Muhammad Usman Qamar Asim Shahzad |
| author_facet | Mohsin Khurshid Muhammad Hidayat Rasool Muhammad Hussnain Siddique Farrukh Azeem Muhammad Naeem Muhammad Sohail Muhammad Sarfraz Muhammad Saqalein Zeeshan Taj Muhammad Atif Nisar Muhammad Usman Qamar Asim Shahzad |
| author_sort | Mohsin Khurshid |
| collection | DOAJ |
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Introduction: The spread of carbapenem-resistant Acinetobacter baumannii (CRAB) is difficult to control especially in the hospitals due to the successful mobilization and evolution of the genetic elements harboring the resistant determinants. The study was conducted to examine the distribution of aminoglycosides, tetracycline, and sulfonamide-resistant determinants among CRAB isolates that carry the blaOXA-23 gene.
Methodology: For a total of 160 CRAB strains isolated at tertiary care hospitals of Pakistan that mainly carried blaOXA-23 gene were included in the study to evaluate the assortment of antibiotic resistance genes.
Results: The susceptibility rates of CRAB for other than beta-lactam drugs were 2.5% for both ciprofloxacin and aminoglycosides and 18% and 25% for sulfonamides and tetracyclines, respectively. Polymyxin B (MIC90, 1 g/mL) Colistin (MIC90, 1 g/mL) and Tigecycline (MIC90, 2 g/mL) were most active against these extensively drug-resistant CRAB isolates. The isolates were found to possess various genes mainly the tetB and sul2 for tetracycline and sulfonamide but the genes conferring resistance to aminoglycosides were varied with various combinations.
Conclusion: Despite the CRAB clones containing blaOXA-23 have been previously reported in Pakistani hospitals, the screening of genetic determinants responsible for other antimicrobial agents is crucial for developing an effective surveillance and mitigation system for infection management.
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| format | Article |
| id | doaj-art-6c3626cb21af4ec59664fed46eaca0fd |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2019-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-6c3626cb21af4ec59664fed46eaca0fd2025-08-20T03:52:43ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802019-10-01131010.3855/jidc.11410Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumanniiMohsin Khurshid0Muhammad Hidayat Rasool1Muhammad Hussnain Siddique2Farrukh Azeem3Muhammad Naeem4Muhammad Sohail5Muhammad Sarfraz6Muhammad Saqalein7Zeeshan Taj8Muhammad Atif Nisar9Muhammad Usman Qamar10Asim Shahzad11Department of Microbiology, Government College University, Faisalabad, PakistanDepartment of Microbiology, Government College University, Faisalabad, PakistanDepartment of Bioinformatics and Biotechnology, Government College University, Faisalabad, PakistanDepartment of Bioinformatics and Biotechnology, Government College University, Faisalabad, PakistanDepartment of Microbiology, Government College University, Faisalabad, PakistanDepartment of Microbiology and Molecular Genetics, University of the Punjab, Lahore, PakistanDepartment of Microbiology and Molecular Genetics, University of the Punjab, Lahore, PakistanDepartment of Microbiology, Government College University, Faisalabad, PakistanDepartment of Microbiology, Government College University, Faisalabad, PakistanDepartment of Microbiology, Government College University, Faisalabad, PakistanDepartment of Microbiology, Government College University, Faisalabad, PakistanDepartment of Pathology, Faculty of Veterinary Sciences, University of Agriculture, Faisalabad, Pakistan Introduction: The spread of carbapenem-resistant Acinetobacter baumannii (CRAB) is difficult to control especially in the hospitals due to the successful mobilization and evolution of the genetic elements harboring the resistant determinants. The study was conducted to examine the distribution of aminoglycosides, tetracycline, and sulfonamide-resistant determinants among CRAB isolates that carry the blaOXA-23 gene. Methodology: For a total of 160 CRAB strains isolated at tertiary care hospitals of Pakistan that mainly carried blaOXA-23 gene were included in the study to evaluate the assortment of antibiotic resistance genes. Results: The susceptibility rates of CRAB for other than beta-lactam drugs were 2.5% for both ciprofloxacin and aminoglycosides and 18% and 25% for sulfonamides and tetracyclines, respectively. Polymyxin B (MIC90, 1 g/mL) Colistin (MIC90, 1 g/mL) and Tigecycline (MIC90, 2 g/mL) were most active against these extensively drug-resistant CRAB isolates. The isolates were found to possess various genes mainly the tetB and sul2 for tetracycline and sulfonamide but the genes conferring resistance to aminoglycosides were varied with various combinations. Conclusion: Despite the CRAB clones containing blaOXA-23 have been previously reported in Pakistani hospitals, the screening of genetic determinants responsible for other antimicrobial agents is crucial for developing an effective surveillance and mitigation system for infection management. https://jidc.org/index.php/journal/article/view/11410CRABsurveillanceaminoglycosidestetracyclinessulfonamides |
| spellingShingle | Mohsin Khurshid Muhammad Hidayat Rasool Muhammad Hussnain Siddique Farrukh Azeem Muhammad Naeem Muhammad Sohail Muhammad Sarfraz Muhammad Saqalein Zeeshan Taj Muhammad Atif Nisar Muhammad Usman Qamar Asim Shahzad Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii Journal of Infection in Developing Countries CRAB surveillance aminoglycosides tetracyclines sulfonamides |
| title | Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii |
| title_full | Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii |
| title_fullStr | Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii |
| title_full_unstemmed | Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii |
| title_short | Molecular mechanisms of antibiotic co-resistance among carbapenem resistant Acinetobacter baumannii |
| title_sort | molecular mechanisms of antibiotic co resistance among carbapenem resistant acinetobacter baumannii |
| topic | CRAB surveillance aminoglycosides tetracyclines sulfonamides |
| url | https://jidc.org/index.php/journal/article/view/11410 |
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